Published

2005-05-01

The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser

Efecto de la concentración de material inerte en un biocatalizador de alginato de calcio con células inmovilizadas sobre la fermentación láctica

DOI:

https://doi.org/10.15446/ing.investig.v25n2.14644

Keywords:

lactic acid, fermentation, biocatalyst, immobilised cells, calcium alginate (en)
lactic acid, fermentation, biocatalyst, immobilised cells, calcium alginate (es)

Authors

  • Juan Carlos Serrato Universidad Nacional de Colombia
  • Luis A Caicedo Mesa Universidad Nacional de Colombia

Colombia is one of the world’s main sugarcane cultivating countries but it has not diversified its fermentation industry; a few fermentation industries produce alcohol and yeasts. Lactic acid and its derivatives then become alternatives providing added value to the sugar produced, thus benefiting the regions producing the sugar. This work evaluated the kinetics of lactic acid production using immobilized cells in calcium alginate at different concentrations of inert material. Lactobacillus delbrueckii was the microorganism used and fermentation broth mainly consisted of sucrose and yeast exact. CSTR reactors were used without pH control. The results suggested that 2% to 3% inert material in the biocatalyst increased cellular retention and diffusiveness, leading to improved conversion and reaction rate.

Colombia es uno de los principales cultivadores mundiales de caña de azúcar y no ha desarrollado una industria de fermentación diversificada, centrándose principalmente en la producción de alcoholes y levaduras. El ácido láctico y sus derivados, constituyen una alternativa que da mayor valor agregado al azúcar producido y con ello se beneficiaria a las regiones que lo producen. En el presente trabajo se evaluó la cinética de producción de ácido láctico utilizando células inmovilizadas en alginato de calcio con un inerte con características zeolíticas en diterentes concentraciones. Se empleó como microorganismo Lactobacillus delbrueckii en un medio compuesto principalmente por sacarosa y extracto de levadura. Para los ensayos se emplearon reactores de mezcla total sin control de pH. Los resultados indican que un porcentaje de inerte entre 2 y 3% mejora la retención celular y la difusividad, produciendo por consiguiente mayores conversiones y velocidades de reacción.

References

Burns, M.; Kwesitadze, J.; Gloves, H., “Dried calcium alginate magnetic spheres for lactic fermentation”, en Biotechnology and Bioengineering, Vol. 27, 1985, pp. 137-145. DOI: https://doi.org/10.1002/bit.260270206

Chronopoulos, G., “Lactic acid fermentation by Lactobacillus casei in free cell form and immobilized on gluten pellets”, en Biotechnology Letters, Vol. 24, 2002, pp 1233-1236. DOI: https://doi.org/10.1023/A:1016274121642

Datta, R. et al., “Technological and economic potential of poly(lactic) acid and lactic acid derivatives”, en FEMS Microbiology Reviews, Vol. 16, 1995, pp 221-231. DOI: https://doi.org/10.1111/j.1574-6976.1995.tb00168.x

Medina, I., “Evaluación de la producción a nivel de banco de ácido láctico por fermentación con células inmovilizadas”, proyecto de grado presentado a la Universidad Nacional de Colombia para optar al título de ingeniero químico, 2001.

Oyaas, J.; Storro, I., “Uptake of lactose and continuous lactic acid fermentation by entrapped non growing Lactobacillus helveticus in whey permeate”, en Applied microbiology and biotechnology, Vol. 46, 1996, pp 240-249, DOI: https://doi.org/10.1007/s002530050811

Senthuran, A. et al., “Lactic acid fermentation in a recycle batch reactor using immobilized Lactobacillus casei”, en Biotechnology and Bioengineering, Vol. 55, 1997, pp 841-853. DOI: https://doi.org/10.1002/(SICI)1097-0290(19970920)55:6<841::AID-BIT3>3.0.CO;2-G

Senthuran, A. et al., “Lactic acid production by immobilized Lactobacillus casei in a recycle batch reactor: a step towards optimization”, en Journal of Biotechnology, Vol. 73, 1999, pp 61-70. DOI: https://doi.org/10.1016/S0168-1656(99)00133-9

Serrato, J. C., “Estudio cinético de la fermentación láctica en un reactor de lecho fijo con recirculación”, tesis presentada a la Universidad Nacional de Colombia para optar al título de magíster en ingeniería química, 2004.

Tango, M.; Ghaly, A., “A continuous lactic acid production system using an immobilized packed bed of Lactobacillus helveticus”, en Applied Microbiology and Biotechnology, Vol. 58, 2002, pp. 712-720. DOI: https://doi.org/10.1007/s00253-002-0970-3

Tuli, A.; Khana, PR y Marwaha, S., “Lactic acid production from whey permeate by immobilized Lactobacillus casei”, en Enzyme and microbial technology, Vol. 7, 1985, pp. 164-168. DOI: https://doi.org/10.1016/0141-0229(85)90058-4

Wang, H.; Seki, M.; Furusaki, S., “Mass transfer behavior in lactic acid fermentation using immobilized Lactobacillus delbrueckii”, en Journal of chemical engineering of Japan, Vol. 28, 1995, pp. 480-482. DOI: https://doi.org/10.1252/jcej.28.480

Yabannavar, V. Wang,; D., “Analysis of mass transfer for immobilized cells in extractive lactic acid fermentation”, en Biotechnology and Bioengineering, Vol. 37, 1991, pp. 544-550. DOI: https://doi.org/10.1002/bit.260370608

Yoo, I. et al., “Encapsulation of Lactobacillus casei in liquid core alginate capsules for lactic acid production”, en Enzyme and microbial technology, Vol. 19, 1996, pp. 428-433. DOI: https://doi.org/10.1016/S0141-0229(96)00016-6

How to Cite

APA

Serrato, J. C. and Caicedo Mesa, L. A. (2005). The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser. Ingeniería e Investigación, 25(2), 78–82. https://doi.org/10.15446/ing.investig.v25n2.14644

ACM

[1]
Serrato, J.C. and Caicedo Mesa, L.A. 2005. The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser. Ingeniería e Investigación. 25, 2 (May 2005), 78–82. DOI:https://doi.org/10.15446/ing.investig.v25n2.14644.

ACS

(1)
Serrato, J. C.; Caicedo Mesa, L. A. The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser. Ing. Inv. 2005, 25, 78-82.

ABNT

SERRATO, J. C.; CAICEDO MESA, L. A. The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser. Ingeniería e Investigación, [S. l.], v. 25, n. 2, p. 78–82, 2005. DOI: 10.15446/ing.investig.v25n2.14644. Disponível em: https://revistas.unal.edu.co/index.php/ingeinv/article/view/14644. Acesso em: 19 apr. 2024.

Chicago

Serrato, Juan Carlos, and Luis A Caicedo Mesa. 2005. “The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser”. Ingeniería E Investigación 25 (2):78-82. https://doi.org/10.15446/ing.investig.v25n2.14644.

Harvard

Serrato, J. C. and Caicedo Mesa, L. A. (2005) “The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser”, Ingeniería e Investigación, 25(2), pp. 78–82. doi: 10.15446/ing.investig.v25n2.14644.

IEEE

[1]
J. C. Serrato and L. A. Caicedo Mesa, “The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser”, Ing. Inv., vol. 25, no. 2, pp. 78–82, May 2005.

MLA

Serrato, J. C., and L. A. Caicedo Mesa. “The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser”. Ingeniería e Investigación, vol. 25, no. 2, May 2005, pp. 78-82, doi:10.15446/ing.investig.v25n2.14644.

Turabian

Serrato, Juan Carlos, and Luis A Caicedo Mesa. “The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser”. Ingeniería e Investigación 25, no. 2 (May 1, 2005): 78–82. Accessed April 19, 2024. https://revistas.unal.edu.co/index.php/ingeinv/article/view/14644.

Vancouver

1.
Serrato JC, Caicedo Mesa LA. The effect on lactic fermentation of concentrating inert material with immobilized cells in a calcium alginate biocatalyser. Ing. Inv. [Internet]. 2005 May 1 [cited 2024 Apr. 19];25(2):78-82. Available from: https://revistas.unal.edu.co/index.php/ingeinv/article/view/14644

Download Citation

CrossRef Cited-by

CrossRef citations0

Dimensions

PlumX

Article abstract page views

338

Downloads

Download data is not yet available.