Automatic segmentation and identification of the Mycobacterium tuberculosis in sputum images
Segmentación e identificación automática del Mycobacterium tuberculosis en imágenes de esputo
DOI:
https://doi.org/10.15446/ing.investig.v23n3.14707Keywords:
color segmentation, fluorescense microscopy, image processing, pattern recognition, tuberculosis (en)segmentación en color, procesamiento de imágenes, microscopia de fluorescencia, reconocimiento de patrones, tuberculosis (es)
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Tuberculosis is a very serious disease whose control is based on early diagnosis. A method frequently employed in its diagnosis consists of the sputum analysis in order to detect the Mycobacterium tuberculosis. The sputum examination demands a great amount of time and a good training of the specialist is required to avoid to commit a great numbers of errors. Image processing techniques can be helpful in examinations. Thus, this paper presents a new technique, it tries to improve the precision and diminish the time used in the analysis sputum samples. This techniques uses the linguistic knowledge about the characteristics of the bacilli, using the color information for segmentation and a classification tree for bacilli identification to establishing if a sample is positive or negative.
La tuberculosis es una grave enfermedad cuyo control está basado en el diagnóstico presuntivo. Un método frecuentemente utilizado para su diagnóstico es el análisis del esputo con el objetivo de detectar el Mycobacterium tuberculosis. El examen del esputo ocupa una gran cantidad de tiempo y se requiere un buen entrenamiento del especialista para evitar cometer un gran número de errores. Las técnicas de procesamiento de imágenes pueden ser de gran ayuda para realizar un examen. Así, se presenta una nueva técnica que pretende mejorar la precisión y disminuir el tiempo empleado en el análisis de muestras de esputo. Esta técnica emplea el conocimiento lingüístico acerca de las características de los bacilos, utilizando la información de color para la segmentación y un árbol de clasificación para la identificación de los bacilos con el fin de establecer si una muestra es positiva o negativa.
References
Veropoulus, K.; Learmonth, G.; Campbell, C.; Knight, B.; Simpson, J. (1999), Automatic identification of tubercle bacilli in sputum. A preliminary investigation. Analytical and Quantitative Cytology and Histology 21(4), p. 277-281.
Veropoulos, K.; Campbell, C.; Learmonth, G.; Knight, B.; Simpson, J. (1998). The automatic identification of tubercle bacilli using image processing and neural computing techniques, en Proceeding of the 8th International Conference on Artificial Neural Networks. 2, p. 797-802, DOI: https://doi.org/10.1007/978-1-4471-1599-1_123
Wilkinson, M. (1996). Rapid automatic segmentation of fluorescent and phase-contrast images of bacteria, en Fluorescence Microscopy and Fluorescent Probes. New York: Ed. J. Slavik, Plenum Press. DOI: https://doi.org/10.1007/978-1-4899-1866-6_40
Álvarez-Borrego, J.; Mourino, R.; Cristobal, G.; Pech, J. (2000). Invariant optical color correlation for recognition of vibrio cholerae O1. Int. Cont. on Pattern Recognition, 2847, p. 283-286, Barcelona, España.
Demantova, P; Sakamoto, D.; Ioshii, S.; Gamba, H. (2001). Seqmentacao automática de bactérias para o método DEFT, en Proceedings of the II Latin American Congress on Biomedical Engineering. Havana, Cuba.
Sammouda, R.; Niki, N.; Nishitani, H.; Nakamura, 5.; Mori, S, (1997). Segmentation of sputum color image for lung cancer diagnosis, en Int. Cont on image Processing. 1, p, 243-246, Washington, USA.
Sarmmouda, R.; Niki, N.; Nishitani, H.; Kyokage, E. (1998). Segmentation of sputum color image for lung cancer diagnosis based on neural network, IEICE Transactions on Information and Systems, E81-D (8), p. 862-871.
Forero, M.; Sierra, E.; Alvarez, J; Pech, J.; Cristobal, G.; Alcalá, L.; Desco, M. (2001). Automatic sputum color segmentation for tuberculosis diagnosis, en Algorithms and Systems for Optical Information Processing, p. 251-261.
Forero, M.; Sroubek, F; Alvarez, J.; Malpica, N.; Cristobal, G.; Santos, A.; Alcalá, L.; Desco, M.; Cohen, L. (2002), Segmentation, autofocusing and signature extraction of tuberculosis sputum images, en Photonic Devices and Algorithms for Computing, p. 171-182.
Forero, M. (2002). Introducción al procesamiento digital de imágenes. Bogotá: Ed, M.G, Forero.
Cortijo, F. (2001). Técnicas supervisadas II; Aproximación no paramétrica. Rep. Tec. URL: http://www-etsi2.ugr.es/depar/ccia/rf/www/, Universidad de Granada.
Cortijo, F (2001), Técnicas no supervisadas: Métodos de agrupamiento.
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Copyright (c) 2003 Manuel Guillermo Forero
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