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Obtención de fibras y gránulos de carbono para la inmovilización de enzimas
Se prepararon fibras y gránulos de carbono a partir de brea de carbón. Previa filtración, estabilización a 330°C y atmosfera de nitrógeno. Las fibras se obtuvieron por extrusión y los gránulos por inyección sobre agua. La inmovilización de lactasa por adsorción mostró que los gránulos de carbono fueron el soporte más adecuado, teniendo como criterios la menor caída de presión y la mayor capacidad de inmovilización. Los gránulos de carbono fueron caracterizados obteniéndose una densidad de partícula de 2,407 g/cm3, una porosidad de 81,69% y un tamaño de partícula de 3 mm.
Fibers and pellets of carbon were prepared from coal tar. The tar was filtrated and stabilizated in a nitrogen atmosphere at 330°C. The fibers were prepared by extrusion and pellets by injection on water. Lactase was immobilized by adsorption process. Pellets were better support than fibers, because produced lower pressure drop and upper enzyme retention. Pellets showed the following characteristics: density 2,407 g/cm3, porosity 81,69% and diameter 3 mm.
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Copyright (c) 2001 Martha L. Malagón M., Yolanda Rico R., Helda A. de López, Luis Alfonso Caicedo M.
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