Perfiles electroforéticos de las proteínas de semilla de pinos como caracteres taxonómicos
Pine seed protein electrophoretic profiles as taxonomic characters
Keywords:
Proteínas de semilla, taxonomía de Pinus, proteínas de reserva (es)Seed proteins, Pinus taxonomy, reserve proteins (en)
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Las proteínas de semilla o de reserva son sustancias que juegan un papel importante durante la germinación. Su composición es muy conservada dentro de un grupo taxonómico de plantas, por lo que la determinación de los patrones electroforéticos de estas proteínas son útiles en estudios agronómicos, fisiológicos, taxonómicos, moleculares y en estudios de fitopatología. En este trabajo se realizó la caracterización electroforética, en geles de acrilamida, de las proteínas de semilla de cinco especies de pinos del Estado de Durango (Pinus cembroides, P. ayacahuite, E durangensis, P engelmanii y P. cooperi) y uno de Zacatecas (P. maximartinezii), México, para determinar su utilidad como caracteres bioquímicos en taxonomía. Los resultados obtenidos permiten sugerir la validez de los perfiles electroforéticos de proteínas de reserva como marcadores taxonómicos a nivel subgenérico y específico, ya que los perfiles de las tres especies del subgénero Strobus (P. maximartinezii, P cembroides y P ayacahuite) presentan perfiles semejantes entre sí pero claramente diferentes a los perfiles de las tres especies del subgénero Pinus (P durangensis, P engelmanii y P. cooperi). A su vez, los perfiles de cada una de las seis especies son especie-específicos sin presentar variabilidad intrapoblacional.
Seed proteins play a very important role in germination. Seeds from a single taxon have a highly stable composition of storage protein. This stability is useful in agronomic, ecological, physiological, taxonomic, molecular and phyto-pathological studies. This paper describes the electrophoretic characterisation in acrylamide gels of seed protein from five pine species (Pinus cembroides, P ayacahuite, P durangensis, P engelmanii, P cooperi and P maximartinezii) from México to determine the importance of their protein profile as biochemical markers in taxonomy. The results suggest that pine reserve protein electrophoretic profiles have chemical attributes having taxonomic importance at subgenus and species level.
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