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Biodegradación de polietilenglicol 400 con consorcio de Pseudomonas sp. y Rhizobium trifolii
Biodegradation of polyethylegeglycol 400 with consortium of Pseudomonas sp. and Rhizobium trifolii
DOI:
https://doi.org/10.15446/rev.colomb.biote.v25n1.91128Keywords:
consorcio, reactor Batch, cinética, biodegradación (es)consortium, Batch reactor, kinetics, Biodegradation (en)
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Se evaluó la biodegradación de polietilenglicol de peso molecular 400 - PEG 400 con el consorcio Pseudomonas sp. y Rhizobium trifolii en reactores en Batch; para determinar las condiciones óptimas de operación del reactor se preparó medio con sales mínimas esenciales suplementado con peptonaCSMEP; en estos medios se estimó el comportamiento del consorcio con condiciones de temperatura entre 15-37°C, pH en un rango de 5-9 y concentración de PEG 400 del .1-2%. Con los resultados obtenidos en estos ensayos previos se ensamblaron tres reactores en Batch - BK. Se analizaron 14 muestras del contenido de cada uno de los reactores durante 20 días para medir la biodegradación del PEG 400 usando demanda química de oxígeno - DQO en sistema de digestión cerrado. Se obtuvo una disminución de hasta el 98.5% del poliéter, lo que mostró que la simbiosis fue efectiva para alcanzar una remoción importante del contaminante.
The biodegradation of polyethylene glycol of molecular weight 400 - PEG 400 was evaluated with the consortium Pseudomonas sp. and Rhizobium trifolii in Batch reactors. To determine the optimal operating conditions of the reactor, a medium with minimum essential salts supplemented with CSMEP peptone was prepared; In these media, the behavior of the consortium was estimated with temperature conditions between 15-37°C, pH in a range of 5-9 and PEG 400 concentration of .1-2%. With the results obtained in these previous tests, three Batch - BK reactors were assembled. 14 samples of the contents of each one of the reactors were analyzed during 20 days to measure the biodegradation of PEG 400 using chemical oxygen demand - COD in a closed digestion system. A decrease of up to 98.5% of the polyether was obtained, which showed that the symbiosis was effective to achieve an important removal of the contaminant.
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