Publicado

2017-04-01

Obtención de biopelículas de carragenina suplementadas con nanopartículas de plata sintetizadas biológicamente

Preparation of carrageenan biofilms mixed with silver nanoparticles by biological synthesis method

Palabras clave:

anamorfo r1 de Bjerkandera sp, nanopartículas de plata(AgNPs), biopelículas, carragenina kappa, antimicrobiano (es)
Bjerkandera Anamorfo R1 sp, silver nanoparticles (AgNPs), biofilms, kappa carrageenan, antimicrobial properties (en)

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El mejoramiento de las propiedades antimicrobianas en nanopartículas de plata (AgNPs) depende del revestimiento, tamaño y forma, por tanto en la síntesis y producción de estas se hace necesario implementar técnicas que permitan mejorar sus características morfológicas y fisicoquímicas. En esta investigación se sintetizaron AgNPs mediante el hongo ligninolítico anamorfo R1 de Bjerkandera sp, para este caso los pellets (hongo) se pusieron en contacto con la solución 1 mM de nitrato de plata (AgNO3) y se incubaron durante 144 h. Posteriormente, con las AgNPs se prepararon películas de carragenina kappa. Se encontró mediante el análisis de microscopia de barrido (SEM) que las AgNPs no modificaron la estructura del biopolímero (carragenina), formando aglomerados en un rango de 70-300 nm. Estas biopelículas exhibieron fuerte actividad antimicrobiana frente a la bacteria Escherichia coli (E.coli) mostrando que este método de reducción biológica es un proceso innovador para la obtención de biomateriales.
Improving the antimicrobial properties of silver nanoparticles (AgNPs) depends on the coating, size and shape; therefore, it is necessary to implement techniques to increase the morphological and physicochemical characteristics in their synthesis and production. In this research, AgNPs were synthesized by the ligninolytic fungi anamorph R1 of Bjerkandera sp; the fungi’s pellets were mixed with 1 mM solution of silver nitrate (AgNO3) and were incubated for 144 h. Subsequently, kappa carrageenan biofilms mixed with AgNPs were prepared. Scanning electron microscopy (SEM) showed that AgNPs did not modify the structure of the biopolymer (carrageenan), forming agglomerates in a range of 70-300 nm. These biofilms had strong antimicrobial properties against Escherichia coli (E.coli), for that reason, this biological synthesis method is an innovative process for obtaining biomaterials.

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