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Fed-batch production and characterization of polyhydroxybutyrate by Bacillus megaterium LVN01 from residual glycerol
Producción por lote alimentado y caracterización de polihidroxibutirato por Bacillus megaterium LVN01 a partir de glicerol residual
DOI:
https://doi.org/10.15446/dyna.v87n214.83523Palabras clave:
polyhydroxyalkanoates (PHA), biodiesel by-product, biopolymers extraction, PHB characterization, statistical experimental design (en)polihidroxialcanoatos (PHA), subproducto de biodiesel, extracción de biopolímeros, caracterización de PHB, diseño experimental estadístico (es)
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The operating conditions of polyhydroxybutyrate (PHB) production processes are among the factors that most influence yields. In this study, we evaluated PHB production synthesized by Bacillus megaterium LVN01. Batch and fed-batch cultures were used to produce PHB from residual glycerol. For this, dry cell weight (DCW) and PHB productivity were analyzed at a preliminary stage by central composite design using batch systems under different temperature, C/N ratio, and fermentation time conditions. The maximum PHB productivity occurred at 30.8 °C, 44.9 mol mol-1, and 39.9 h. The same conditions were tested for studies in fed-batch culture. Fed-batch experiments were comparable to each other, where the DCW was around 1.9 g L-1, with PHB productivities of 29.5 mg L-1 h-1 and 35.6 mg L-1 h-1 for bioreactors of 5 L and 14 L, respectively. The PHB was characterized by NMR, FTIR, DSC, TGA, and DTG analys
Las condiciones de operación de los procesos de producción de PHA se encuentran entre los factores que más influyen en los rendimientos. En este estudio se evaluó la producción de polihidroxibutirato (PHB) sintetizado por Bacillus megaterium LVN01. Se implementaron cultivos en lotes y lotes alimentados para producir PHB, a partir de glicerol residual. Para esto, en una etapa preliminar, se analizó el peso de las células secas (PSC) y la productividad de PHB mediante un diseño central compuesto, utilizando sistemas de lotes bajo diferentes condiciones de temperatura, relación molar C/N y tiempo de incubación. La productividad máxima de PHB ocurrió a 30,8 ° C, 44,9 mol mol-1 y 39,9 h. Las mismas condiciones se utilizaron para estudios en cultivos por lotes alimentados. Los experimentos en modo lote alimentado fueron comparables entre sí, donde el PSC osciló alrededor de 1,9 g L-1, con productividades de PHB de 29,5 mg L-1 h-1 y 35,6 mg L-1 h-1 para biorreactores de 5 L y 14 L, respectivamente. El PHB se caracterizó mediante análisis de RMN, FTIR, DSC, TGA y DTG.
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