Publicado

2022-05-24

Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel

Alteración en la expresión de genes en la línea celular HepG2 por exposición a material particulado (MP) de diésel

DOI:

https://doi.org/10.15446/dyna.v89n221.99394

Palabras clave:

diesel; particulate matter; organic extraction; genotoxicity; mutagenicity; transcriptomics (en)
diésel; material particulado; extracción orgánica; genotoxicidad; mutagenicidad y transcriptómicas (es)

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Autores/as

Particulate matter (PM) less than 2.5 um emitted by diesel engines is responsible for the morbidity and mortality associated with air pollution. Alternative fuels have been implemented, but their potential toxic effects are still unknown. The objective of this article is to evaluate the in vitro toxicity of the organic extract of particulate matter emitted by a diesel engine powered by two different fuels. Following cell exposure to fossil diesel + 10% ethanol and fossil diesel extracts, mutagenicity, genotoxicity, and differential gene expression were evaluated. The results showed that the organic extracts of diesel PM + 10% ethanol were more biotoxic (p<0.05). More studies are needed to better understand the health effect of PM emissions from biofuel.

El material particulado (MP) menor a 2,5 um emitido por los motores diésel es responsable de la morbilidad y mortalidad asociadas con la contaminación del aire. Se han implementado combustibles alternativos, pero aún se desconocen sus posibles efectos tóxicos. El presente artículo tiene por objetivo evaluar la toxicidad in vitro del extracto orgánico de material particulado emitido por un motor diésel alimentado con dos combustibles diferentes. Luego de la exposición celular a los extractos de diésel fósil + 10% etanol y diésel fósil, se evaluó la mutagenicidad, la genotoxicidad y la expresión diferencial de genes. Los resultados mostraron que los extractos orgánicos del MP de diésel + 10%etanol fueron más biotóxicos (p<0.05). Es necesario llevar a cabo más estudios para entender mejor el efecto sobre la salud de las emisiones de MP proveniente de biocombustible.

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Cómo citar

IEEE

[1]
L. Y. . Orozco-Jiménez, «Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel», DYNA, vol. 89, n.º 221, pp. 110–120, abr. 2022.

ACM

[1]
Orozco-Jiménez, L.Y. , Estrada, V., Cuesta-Castro, D.P. , Isaza, J.P. , Molina, F. , Palacio-B, J. , Álvarez-Orozco, M.E. y Ortiz-Trujillo, I. 2022. Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel. DYNA. 89, 221 (abr. 2022), 110–120. DOI:https://doi.org/10.15446/dyna.v89n221.99394.

ACS

(1)
Orozco-Jiménez, L. Y. .; Estrada, V.; Cuesta-Castro, D. P. .; Isaza, J. P. .; Molina, F. .; Palacio-B, J. .; Álvarez-Orozco, M. E. .; Ortiz-Trujillo, I. . Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel. DYNA 2022, 89, 110-120.

APA

Orozco-Jiménez, L. Y. ., Estrada, V., Cuesta-Castro, D. P. ., Isaza, J. P. ., Molina, F. ., Palacio-B, J. ., Álvarez-Orozco, M. E. . & Ortiz-Trujillo, I. . (2022). Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel. DYNA, 89(221), 110–120. https://doi.org/10.15446/dyna.v89n221.99394

ABNT

OROZCO-JIMÉNEZ, L. Y. .; ESTRADA, V.; CUESTA-CASTRO, D. P. .; ISAZA, J. P. .; MOLINA, F. .; PALACIO-B, J. .; ÁLVAREZ-OROZCO, M. E. .; ORTIZ-TRUJILLO, I. . Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel. DYNA, [S. l.], v. 89, n. 221, p. 110–120, 2022. DOI: 10.15446/dyna.v89n221.99394. Disponível em: https://revistas.unal.edu.co/index.php/dyna/article/view/99394. Acesso em: 4 mar. 2026.

Chicago

Orozco-Jiménez, Luz Yaneth, Veronica Estrada, Diana Paola Cuesta-Castro, Juan Pablo Isaza, Francisco Molina, Jaime Palacio-B, María Elena Álvarez-Orozco, y Isabel Ortiz-Trujillo. 2022. «Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel». DYNA 89 (221):110-20. https://doi.org/10.15446/dyna.v89n221.99394.

Harvard

Orozco-Jiménez, L. Y. ., Estrada, V., Cuesta-Castro, D. P. ., Isaza, J. P. ., Molina, F. ., Palacio-B, J. ., Álvarez-Orozco, M. E. . y Ortiz-Trujillo, I. . (2022) «Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel», DYNA, 89(221), pp. 110–120. doi: 10.15446/dyna.v89n221.99394.

MLA

Orozco-Jiménez, L. Y. ., V. Estrada, D. P. . Cuesta-Castro, J. P. . Isaza, F. . Molina, J. . Palacio-B, M. E. . Álvarez-Orozco, y I. . Ortiz-Trujillo. «Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel». DYNA, vol. 89, n.º 221, abril de 2022, pp. 110-2, doi:10.15446/dyna.v89n221.99394.

Turabian

Orozco-Jiménez, Luz Yaneth, Veronica Estrada, Diana Paola Cuesta-Castro, Juan Pablo Isaza, Francisco Molina, Jaime Palacio-B, María Elena Álvarez-Orozco, y Isabel Ortiz-Trujillo. «Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel». DYNA 89, no. 221 (abril 22, 2022): 110–120. Accedido marzo 4, 2026. https://revistas.unal.edu.co/index.php/dyna/article/view/99394.

Vancouver

1.
Orozco-Jiménez LY, Estrada V, Cuesta-Castro DP, Isaza JP, Molina F, Palacio-B J, Álvarez-Orozco ME, Ortiz-Trujillo I. Alteration in the gene expression of the HepG2 cell line by exposure to particulate matter (PM) of diesel. DYNA [Internet]. 22 de abril de 2022 [citado 4 de marzo de 2026];89(221):110-2. Disponible en: https://revistas.unal.edu.co/index.php/dyna/article/view/99394

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1. Carolina Mendoza, Silvana Arias, Maria L. Botero, John R. Agudelo. (2025). Hazardous gas emissions from drop-in biofuels: mutagenicity, cytotoxicity, and unregulated pollutants. Journal of Hazardous Materials, 483, p.136696. https://doi.org/10.1016/j.jhazmat.2024.136696.

2. Hiu-Lok Ngan, Jialing Zhang, Yi Chen, Yuanyuan Song, Zenghua Qi, Zhu Yang, Hong Yan, Zongwei Cai. (2025). Hepatotoxicity Prediction and Multi-omics Reveal Mitochondrial and Lipid Metabolic Dysregulation in PM2.5-Induced Liver Fibrosis. Environment & Health, https://doi.org/10.1021/envhealth.5c00401.

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