Addition of cyclodextrins saturated with cholesterol and its effect on the cooling of goat semen
Adición de ciclodextrinas saturadas con colesterol y su efecto en la refrigeración de semen caprino
DOI:
https://doi.org/10.15446/rfmvz.v71n3.113280Keywords:
reproduction, caprine, spermatozoa, cryopreservation (en)reproducción, caprinos, espermatozoide, crioconservación (es)
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Caprine spermatozoa exhibit a low cholesterol-to-phospholipid ratio in their plasma membrane, which affects its structure and functionality, as well as sperm survival after the cryopreservation process. The presence and incorporation of cholesterol into the plasma membrane enhances membrane stability, thereby increasing sperm survival. The objective of this study was to evaluate the effect of adding cholesterol-loaded methyl-β-cyclodextrin (CLC) on the viability and membrane integrity of chilled semen. Four Anglo Nubian x Boer crossbred males were used, with 5 ejaculates collected per male, resulting in a total of 20 ejaculates. Each ejaculate was divided into four groups: a control without CLC and three treatment groups (1, 2, and 3 mg of CLC per 120 million spermatozoa). The samples were refrigerated at 5 °C for 24 and 48 hours for evaluation. No statistical differences were observed between the treatment means for semen viability after 24 and 48 hours. However, in treatment 3, a statistically significant decrease in membrane integrity was observed after 24 and 48 hours. It is concluded that, although a biological improvement was observed, it was not statistically significant.
Los espermatozoides caprinos presentan una baja relación colesterol/fosfolípidos en la membrana plasmática, lo que afecta su estructura y funcionalidad, como también la supervivencia del espermatozoide tras el proceso de criopreservación. La presencia de colesterol y su incorporación a la membrana plasmática aumenta su estabilidad, lo cual incrementa la supervivencia de los espermatozoides. El objetivo de este trabajo fue evaluar el efecto de la adición de metil-β-ciclodextrina cargada de colesterol (CLC) sobre la viabilidad e integridad de membrana del semen refrigerado. Se emplearon 4 machos cruza Anglo Nubian x Boer y se obtuvieron 5 eyaculados por macho, con un total de 20 eyaculados. Cada eyaculado fue dividido en cuatro: control sin CLC y tres tratamientos (1, 2 y 3 mg de CLC/120 millones de espermatozoides). Las muestras fueron refrigeradas a 5 °C durante 24 y 48 horas para su evaluación. No hubo diferencias estadísticas entre las medias de los tratamientos para la viabilidad del semen refrigerado luego de 24 y 48 horas. Sin embargo, en el tratamiento 3 se observó una diferencia estadística en la integridad de membrana que disminuyó luego de las 24 y 48 horas. Se concluye que, a pesar de encontrar una mejora desde el punto de vista biológico, no tiene significancia estadística.
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