Published

2018-01-01

Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia

First evidence of Bovine Viral Diarrhea Virus (BVDV) genotype 2 in Colombia

DOI:

https://doi.org/10.15446/rfmvz.v65n1.72020

Keywords:

Diarrea Viral Bovina, VDVB genotipo 2, RT-PCR, cartílago de oreja. (es)
Bovine Viral Diarrhea Virus, BVDV-2, RT-PCR, ear notche. (en)

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Authors

  • V. V. Villamil Grupo de investigación en microbiología y epidemiología, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Sede Bogotá. Carrera 30 No. 45-03. Bogotá, Colombia.
  • G. C. Ramírez Grupo de investigación en microbiología y epidemiología, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Sede Bogotá. Carrera 30 No. 45-03. Bogotá, Colombia.
  • V. J. Vera Grupo de investigación en microbiología y epidemiología, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Sede Bogotá. Carrera 30 No. 45-03. Bogotá, Colombia.
  • J. A. Jaime Grupo de investigación en microbiología y epidemiología, Facultad de Medicina Veterinaria y de Zootecnia, Universidad Nacional de Colombia, Sede Bogotá. Carrera 30 No. 45-03. Bogotá, Colombia.
El VDVB es un patógeno que afecta los hatos bovinos. Ha sido clasificado en 2 biotipos (citopático y no citopático) y en 3 genotipos (1, 2, 3) con base en su secuencia nucleotídica. El propósito del presente estudio fue determinar la presencia del  VDVB genotipo 2 (VDVB-2) en el país evaluando animales procedentes de 4 zonas ganaderas, empleando la técnica  de RT-PCR en muestras de suero y cartílago de oreja. Se recolectaron 379 sueros preparto de hembras bovinas, 274 sueros precalostrales de los terneros nacidos de estas hembras, 145 sueros de los terneros 25 días post-nacimiento y 181 biopsias de cartílago de oreja de los mismos terneros. Inicialmente, se realizó RT-PCR a todas las muestras para determinar la presencia o ausencia del VDVB. Posteriormente, aquellas muestras que resultaron positivas fueron evaluadas mediante 2 métodos para determinar su genotipo: a) una nueva RT-PCR con primers específicos para el VDVB-2  y  b) otra PCR obteniendo un producto de 296 pb el cual fue sometido a digestión enzimática. Se encontró que 17 (4,48%) muestras de suero de hembras resultaron positivas a Pestivirus de las cuales 6 correspondían al VDVB-2 (1,58%). Ninguna de las muestras de suero obtenidas de los terneros resultó positiva  para el VDVB-2. Finalmente, 18 (9,9%) biopsias de cartílago de oreja fueron positivas al VDVB, siendo 14 de estas (7,7%) positivas para el VDVB-2. Este estudio representa la primera evidencia documentada de la presencia del genotipo 2 del VDVB en bovinos de Colombia.

Bovine viral diarrhea virus (BVDV) is a disease that affects cattle herds. The virus has been classified into two biotypes (cytopathic and noncytopathic) and three genotypes (1, 2, 3) according to their nucleotide sequence. The purpose of this study was to determine the presence of BVDV genotype 2 (BVDV-2) in the country, evaluating four representative areas of cattle production using RT-PCR on serum and ear notches. For this, 379 prepartum cattle’s sera, 274 precolostral sera from calves born to these cows and 145 calves’s (25 post-birth days) sera were collected. Additionally, 181 ear notches of these calves were taken. Subsequently, extraction of viral RNA from samples was performed. Initially RT-PCR was performed on all samples to determine the presence or absence of BVDV; samples that tested positive were assessed by two methods to determine their genotype: a) a new RT-PCR with specific primers for BVDV-2, b) another PCR obtaining a product of 296 bp which was subjected to enzymatic digestion. It was found that 17 (4.48%) prepartum cattle’s sera samples were positive for Pestiviruses, of which 6 corresponded to BVDV-2 (1.58%). None of the sera obtained from the calves were positive for BVDV-2. Finally, 18 (9.9%) ear notches were positive for BVDV, 14 (7.7%) of which were positive for BVDV-2. This study represents the first documented evidence of the presence of the BVDV-2 in cattle from Colombia.

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Villamil, V. V., Ramírez, G. C., Vera, V. J. and Jaime, J. A. (2018). Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia. Revista de la Facultad de Medicina Veterinaria y de Zootecnia, 65(1), 11–26. https://doi.org/10.15446/rfmvz.v65n1.72020

ACM

[1]
Villamil, V.V., Ramírez, G.C., Vera, V.J. and Jaime, J.A. 2018. Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia. Revista de la Facultad de Medicina Veterinaria y de Zootecnia. 65, 1 (Jan. 2018), 11–26. DOI:https://doi.org/10.15446/rfmvz.v65n1.72020.

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Villamil, V. V.; Ramírez, G. C.; Vera, V. J.; Jaime, J. A. Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia. Rev. Med. Vet. Zoot. 2018, 65, 11-26.

ABNT

VILLAMIL, V. V.; RAMÍREZ, G. C.; VERA, V. J.; JAIME, J. A. Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia. Revista de la Facultad de Medicina Veterinaria y de Zootecnia, [S. l.], v. 65, n. 1, p. 11–26, 2018. DOI: 10.15446/rfmvz.v65n1.72020. Disponível em: https://revistas.unal.edu.co/index.php/remevez/article/view/72020. Acesso em: 2 mar. 2025.

Chicago

Villamil, V. V., G. C. Ramírez, V. J. Vera, and J. A. Jaime. 2018. “Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia”. Revista De La Facultad De Medicina Veterinaria Y De Zootecnia 65 (1):11-26. https://doi.org/10.15446/rfmvz.v65n1.72020.

Harvard

Villamil, V. V., Ramírez, G. C., Vera, V. J. and Jaime, J. A. (2018) “Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia”, Revista de la Facultad de Medicina Veterinaria y de Zootecnia, 65(1), pp. 11–26. doi: 10.15446/rfmvz.v65n1.72020.

IEEE

[1]
V. V. Villamil, G. C. Ramírez, V. J. Vera, and J. A. Jaime, “Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia”, Rev. Med. Vet. Zoot., vol. 65, no. 1, pp. 11–26, Jan. 2018.

MLA

Villamil, V. V., G. C. Ramírez, V. J. Vera, and J. A. Jaime. “Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia”. Revista de la Facultad de Medicina Veterinaria y de Zootecnia, vol. 65, no. 1, Jan. 2018, pp. 11-26, doi:10.15446/rfmvz.v65n1.72020.

Turabian

Villamil, V. V., G. C. Ramírez, V. J. Vera, and J. A. Jaime. “Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia”. Revista de la Facultad de Medicina Veterinaria y de Zootecnia 65, no. 1 (January 1, 2018): 11–26. Accessed March 2, 2025. https://revistas.unal.edu.co/index.php/remevez/article/view/72020.

Vancouver

1.
Villamil VV, Ramírez GC, Vera VJ, Jaime JA. Primera evidencia del Virus de Diarrea Viral Bovina (VDVB) genotipo 2 en Colombia. Rev. Med. Vet. Zoot. [Internet]. 2018 Jan. 1 [cited 2025 Mar. 2];65(1):11-26. Available from: https://revistas.unal.edu.co/index.php/remevez/article/view/72020

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