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REDUCED GRAPHENE OXIDE NANOSHEETS DERIVED FROM IRAQI RHUS CORIARIA (L.) FRUITS WERE EVALUATED FOR THEIR ANTICANCER AND ANTIBACTERIAL PROPERTIES
EVALUACIÓN DE LAS LÁMINAS DE NANÓXIDO DE GRAFENO REDUCIDO DERIVADAS DE LOS FRUTOS DE RHUS CORIARIA (L).DE IRAK POR SUS PROPIEDADES ANTICANCERÍGENAS Y ANTIBACTERIANAS
DOI:
https://doi.org/10.15446/rev.fac.cienc.v13n1.110790Palabras clave:
Iraqi Rhus coriaria (L.), MCF-7 breast cancer, mechanism of bioreduction rGO, reduced graphene oxide (rGO). (en)Descargas
In this study, reduced graphene oxide nanosheets (rGO) were biosynthesized using the methanolic extract of Iraqi Rhus coriaria (L.) fruits with substantial bioreduction capabilities. The GC-MS analysis of the methanolic extract of Iraqi Rhus coriaria (L.) fruits was used to determine the synthesis mechanism of rGO nanosheets. The synthesized graphene oxide (GO) and the biosynthesized methanolic extract of Iraqi Rhus coriaria (L.) fruits-rGO nanosheets (MERCF-rGO) were characterized using UV-Vis at 226 nm, and 238 nm, respectively. FTIR explained the functional groups of GO and MERCF-rGO nanosheets, XRD spectra of the GO and MERCF-rGO show the sizes at 10.42 nm, and 4.07 nm, TEM observed the size of GO and MERCF-rGO at 15.54 nm and 9.6 nm. As well as FESEM of GO and MERCF-rGO was carried out to explain sheet shape about them. Raman spectroscopy of GO and MERCF-rGO displayed the two peaks D at (1353 cm-1, 1336 cm-1) and G at (1597 cm-1, 1594 cm-1) respectively. MERCF-rGO nanosheets showed activity against antibacterial gram-positive (Staphylococcus aureus) and gram-negative (Escherichia coli), cytotoxicity by MTT assay against breast cancer MCF-7 cell line IC50 at 251.99 μg/mL, genotoxicity fragmented DNA of MCF-7 by comet assay. Comprehensively, the green synthesis of rGO is safe, the lowest cost and developable biogenic nano-formulation of Iraqi Rhus coriaria (L.)–rGO owns antibacterial and anticancer therapeutic applications.
En este estudio, se biosintetizaron nanohojas de óxido de grafeno reducido (rGO) utilizando el extracto metanólico de frutos iraquíes de Rhus coriaria (L.) con importantes capacidades de biorreducción. El análisis GC-MS del extracto metanólico de los frutos iraquíes de Rhus coriaria (L.) se utilizó para determinar el mecanismo de síntesis de las nanohojas de rGO. El óxido de grafeno sintetizado (GO) y el extracto metanólico biosintetizado de nanohojas de frutos-rGO de Rhus coriaria (L.) iraquí (MERCF-rGO) se caracterizaron utilizando UV-Vis a 226 nm y 238 nm, respectivamente. FTIR explicó los grupos funcionales de las nanohojas GO y MERCF-rGO, los espectros XRD de GO y MERCF-rGO muestran los tamaños a 10,42 nm y 4,07 nm, TEM observó el tamaño de GO y MERCF-rGO a 15,54 nm y 9,6 nm. Además, se llevó a cabo FESEM de GO y MERCF-rGO para explicar la forma de las hojas. La espectroscopia Raman de GO y MERCF-rGO mostró los dos picos D en (1353 cm-1, 1336 cm-1) y G en (1597 cm-1, 1594 cm-1) respectivamente. Las nanohojas MERCF-rGO mostraron actividad contra antibacterianos grampositivos (Staphylococcus aureus) y gramnegativos (Escherichia coli), citotoxicidad mediante ensayo MTT contra cáncer de mama, línea celular MCF-7 IC50 a 251,99 μg/mL, genotoxicidad ADN fragmentado de MCF-7 por ensayo de cometa. En términos generales, la síntesis verde de rGO es segura, la nanoformulación biogénica desarrollable y de menor costo de Rhus coriaria (L.) iraquí; rGO posee aplicaciones terapéuticas antibacterianas y anticancerígenas.
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