Publicado

2013-05-01

MOLECULAR PHYLOGENY OF THE NERITIDAE (GASTROPODA: NERITIMORPHA) BASED ON THE MITOCHONDRIAL GENES CYTOCHROME OXIDASE I (COI) AND 16S rRNA

Filogenia molecular de la familia Neritidae (Gastropoda: Neritimorpha) con base en los genes mitocondriales citocromo oxidasa I (COI) y 16S rRNA

Palabras clave:

Colombian Caribbean, mDNA, Nerita, Neritina, radiation, mitochondrial genes (en)
Nerita, Neritina, Caribe colombiano, genoma mitocondrial, ADNmt, radiación. (es)

Autores/as

  • Julián Fernando Quintero Galvis Universidad del Magdalena
  • Lyda Raquel Castro Universidad del Magdalena

The family Neritidae has representatives in tropical and subtropical regions that occur in a variety of environments, and its known fossil record dates back to the late Cretaceous. However there have been few studies of molecular phylogeny in this family. We performed a phylogenetic reconstruction of the family Neritidae using the COI (722 bp) and the 16S rRNA (559 bp) regions of the mitochondrial genome. Neighbor-joining, maximum parsimony and Bayesian inference were performed. The best phylogenetic reconstruction was obtained using the COI region, and we consider it an appropriate marker for phylogenetic studies within the group. Consensus analysis (COI +16S rRNA) generally obtained the same tree topologies and confirmed that the genus Nerita is monophyletic. The consensus analysis using parsimony recovered a monophyletic group consisting of the genera NeritinaSeptariaTheodoxusPuperita, and Clithon, while in the Bayesian analyses Theodoxus is separated from the other genera. The phylogenetic status of the species from the genus Nerita from the Colombian Caribbean generated in this study was consistent with that reported for the genus in previous studies. In the resulting consensus tree obtained using maximum parsimony, we included information on habitat type for each species, to map the evolution by habitat. Species of the family Neritidae possibly have their origin in marine environments, which is consistent with conclusions from previous reports based on anatomical studies.

La familia Neritidae cuenta con representantes en regiones tropicales y subtropicales adaptadas a diferentes ambientes, con un registro fósil que data para finales del Cretáceo. Sin embargo no se han realizado estudios de filogenia molecular en la familia. En este estudio se realizó una reconstrucción filogenética de la familia Neritidae utilizando las regiones COI (722 pb) y 16S rRNA (559 pb) del genoma mitocondrial. Se realizaron análisis de distancias de Neighbor-Joining, Máxima Parsimonia e Inferencia Bayesiana. La mejor reconstrucción filogenética fue mediante la región COI, considerándola un marcador apropiado para realizar estudios filogenéticos dentro del grupo. El consenso de las relaciones filogenéticas (COI+16S rRNA) permitió confirmar que el género Nerita es monofilético. El consenso del análisis de parsimonia reveló un grupo monofilético formado por los géneros NeritinaSeptariaTheodoxusPuperita y Clithon, mientras que en el análisis bayesiano Theodoxus se encuentra separado de los otros géneros. El resultado en las especies del género Nerita del Caribe colombiano fue consistente con lo reportado para el género en estudios previos. En el árbol resultante del análisis de parsimonia se sobrepuso la información del hábitat de cada especie, para mapear la evolución por hábitat. Se obtuvo como resultado que las especies de la familia Neritidae posiblemente tengan su origen en un ambiente marino, siendo congruente con lo reportado en estudios anatómicos realizados anteriormente.

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Citas

Aktipis SW, Giribet G. A phylogeny of Vetigastropoda and other "archaeogastropods": re-organizing old gastropod clades. Invertebr Biol. 2010;129(3):220-240.

Bandel K. Operculum shape and construction of some fossil Neritimorpha (Gastropoda) compared to those of modern species of the subclass. Vita Malacologica.2008;7:19-36.

Blanco JF, Scatena FN. Hierarchical contribution of river - ocean connectivity, water chemistry, hydraulics, and substrate to the distribution of diadromous snails in Puerto Rican streams. JN Am Benthol Soc. 2006;25(1):82-98.

Bunje PME. Fluvial range expansion , allopatry, and parallel evolution in a Danubian snail lineage (Neritidae: Theodoxus). Biol J Linn Soc. 2007;90(4):603-617.

Bunje PME, Lindberg DR. Lineage divergence of a freshwater snail clade associated with post-Tethys marine basin development. Mol Phylogenet Evol. 2007;42(2):373-87.

Castro LR, Colgan DJ. The phylogenetic position of Neritimorpha based on the mitochondrial genome of Nerita melanotragus (Mollusca: Gastropoda). Mol Phylogenet Evol. 2010;57 (2):918-23.

Díaz JM, Puyana M. Familia Neritidae. In: Díaz JM, Puyana M. Moluscos del Caribe Colombiano. INVEMAR, COCIENCIAS, Fundación Natura Colombia. Santa Fe de Bogotá; 1994. p. 122-124.

Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Mol Mar Biol Biotech. 1994;3(5):294-299.

Frey M. A revised classification of the gastropod genus Nerita. The Veliger. 2010a;51(1):1-7.

Frey M. The relative importance of geography and ecology in species diversification evidence from a tropical marine intertidal snail Nerita. J Biogeogr. 2010b;37(8):1515-1528.

Frey M, Vermeij GJ. Molecular phylogenies and historicalbiogeography of a circumtropical group of gastropods (Genus: Nerita): implications for regional diversity patterns in the marine tropics. Mol Phylogenet Evol. 2008;48(3):1067-1086.

Glor RE. Phylogenetic insights on adaptive radiation. Annu.Rev. Ecol. Evol. Syst. 2010;41:251-270.

Gorbach KR, Benbow ME, Mcintosh MD, Burky AJ. Dispersal and upstream migration of an amphidromous neritid snail: implications for restoring migratory pathways in tropical streams. Freshwater Biol. 2012;57(8):1643-1657.

Hall TA. BioEdit: a user-friendly biological sequences aligment editor and analysis program for Window 95/98/NT. Nucl Acid S. 1999;41:95-98.

Holthuis BV. Evolution between marine and freshwater habitats: a case study of the gastropod Neritopsina [PhD thesis]. University of Washington, DC; 1995. p. 249.

Huelsenbeck JP, Ronquist F. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001;17(8):754-755.

Hurtado LA, Frey M, Gaube P, Pfeiler E, Markow TA. Geographical subdivision, demographic history and geneflow in two sympatric species of intertidal snails, Nerita scabricosta and Nerita funiculata, from the tropical eastern Pacific. Mar Biol. 2007;151(5):1863-1873.

Källersjö M, Albert VA, Farris JS. Homoplasy increases phylogenetic structure. Cladistics. 1999;15 (1):91-93.

Kano Y. Usefulness of the opercular nucleus for inferring early development in Neritimorph Gastropods. J Morphol. 2006;267(9):1120-1136.

Kano Y. Hitchhiking behaviour in the obligatory upstream migration of amphidromous snails. Biol Letters. 2009; 5(4):465-468.

Kano Y, Chiba S, Kase T. Major adaptive radiation in Neritopsine gastropods estimated from 28S rRNA sequences and fossil records. T Roy Soc. 2002;269(1508):2457-2465.

Kano Y, Strong EE, Fontaine B, Gargominy O, Glaubrech M, Bouchet P. Focus on freshwater snail. In: Bouchet H, Guyader H, Pascal O. The natural history of Santo. Paris; 2006. p. 257-264.

Klussmann-Kolb A, Dinapoli A, Kuhn K, Streit B, Albrecht C. From sea to land and beyond-new insights into the evolution of Euthyneuran Gastropoda (Mollusca). BMC Evol Biol. 2008;8(1):57-73

Nei M, Gojobori T. Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol. 1986;3(5):418-426.

Nylander J. MrModeltest. Program distributed by the author, Evolutionary Biology Centre. Uppsala University. 2004.

Palumbi SR. Nucleic acids, II: the polymerase chain reaction. In: Hill DM, Morizt C, Mable BK. (Eds.). Molecular Systematics. Massachusetts: Sinauer Associates, Sunderland; 1996. p. 205-247.

Ponder WF. Superorder Neritopsina. In: Beesley P, Ross G, Well A (Eds.). Mollusca: the Southern Synthesis. Fauna of Australia. CSIRO Publishing, Melbourne; 1998. p. 693-702.

Ponder WF, Lindberg DR. Towards a phylogeny of Gastropod Molluscs: An analysis using morphological characters. Zool J Linn Soc-Lond. 1997;119(2):83-265.

Posada BO, Rozo D, Bolaños J, Zamora A. In: INVEMAR Informe del Estado de los Ambientes y Recursos Marinos y Costeros en Colombia Año 2009. Serie de Publicaciones Periódicas No. 8. Santa Marta; 2010. p. 25-32.

Pybus O, Shapiro B. Natural selection and adaptation of molecular sequences. In: Lemey P, Salemi M, Vandamme AM (Eds.). The Phylogenetic Handbook. A practical approach to phylogenetic analysis and hypothesis testing. Cambridge. New York, Madrid; 2009. p. 415.

Remigio EA, Hebert PDN. Testing the utility of partial COI sequences for phylogenetic estimates of Gastropod relationships. Mol Phylogenet Evol. 2003;29(3):641-647.

Ronquist F, Huelsenbeck JP. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003;19 (12):1572-1574.

Russell HD. The recent mollusk of the familily Neritidae of Western Atlantic. Bull. Mus. Comp. Zool; 1941. p. 347-404.

Swofford D. PAUP* Phylogenetic analysis using parsimony (*and other methods), version 4. Sinauer Associates, Sunderland, MA. 2002.

Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood , evolutionary distance , and maximum parsimony methods research resource. Mol Biol Evol. 2011;28(10):2731-2739.

Thompson FG. Proserpinoid land snails and their relationships within the Archaeogastropoda. Malacologia. 1980;20 (1): 1-33.

Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997;25(24):4876-4882.

Xia X. Assessing substitution saturation with Dambe. In Lemey P, Salemi M, Vandamme AM (Eds.). The Phylogenetic Handbook. A practical approach to phylogenetic analysis and hypothesis testing. Cambridge. New York, Madrid; 2009. p. 617-630.

Xia X, Xie Z. DAMBE: Software package for data analysis in molecular biology and evolution. J Hered. 2001(4); 92:371-373.

Yidi E, Sarmiento V. Colombian seashells from the Caribbean sea. Piceno, L Informatore; 2010. p. 1-384.

Zande J, Carney R. Population size structure and feeding biology of Bathynerita naticoidea Clarke 1989 (Gastropod: Neritacea) from Gulf of Mexico hydrocarbon seeps. Gulf Mex Sci. 2001;19 (2):107-118.