Publicado

2015-01-01

EFFECT OF FOLIAR APPLICATION OF AMINOACIDS ON PLANT YIELD AND SOME PHYSIOLOGICAL PARAMETERS IN BEAN PLANTS IRRIGATED WITH SEAWATER

Efecto de la aplicación foliar de aminoácidos sobre el rendimiento y parámetros fisiológicos en plantas de haba irrigadas con agua de mar

Palabras clave:

free amino acids, photosynthetic pigments, proline, salinity, Vicia faba (en)
aminoácidos libres, pigmentos fotosintéticos, prolina, salinidad, Vicia faba (es)

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Autores/as

  • Mervat SH SADAK National Research Centre - Botany Department
  • Magdi T. ABDELHAMID National Research Centre - Botany Department
  • Urs SCHMIDHALTER Technische Universität München - Institute of Plant Nutrition - Department of Plant Sciences

Salinity decreases yield in arid and semi-arid areas. With increasing demand for irrigation water, alternative sources are being sought. Seawater salinity was previously considered unusable for irrigation. However, this water can be used successfully to grow crops under certain conditions. Amino acids is well known biostimulant which has positive effects on plant growth and yield, and significantly mitigates the injuries caused by abiotic stresses. Therefore, in the present study, the effect of exogenously treatment amino acid on faba bean plant growing under seawater salt stress was investigated. Reduction of salinity damage in faba bean by using a mixture of amino acids to improve morphological and biochemical parameters, and thus raising the level of plant yield was tested. A pot experiment was conducted to alleviate the harmful effects of seawater salinity on faba bean cv. Giza 843 by foliar spraying of an amino acid mixture with different concentrations (0.0, 500, 1000 or 1500 mg L-1). Irrigation of faba bean plants with seawater levels of 3.13 and 6.25 dS m-1 led to significant reductions in shoot length, number of leaves per plant, fresh and dry weight of shoots, photosynthetic pigments, total carbohydrates, polysaccharides, nucleic acid DNA and RNA contents of faba bean leaves. Seawater salinity induced higher contents of Na+ and Cl- and decreased contents of K+, K+:Na+, Ca2+, Mg2+ and P3+. Irrigation of faba bean plant with different levels of seawater decreased seed yield and total dry weight per plant compared with those irrigated with tap water. Also, total carbohydrates and total protein contents in seeds were reduced by increased seawater salinity levels. Amino acid application as foliar spray significantly improved all the reduced parameters due to seawater stress. However, the highest level of amino acid of 1500 mg L-1 exerted the strongest effect in alleviating the harmful effect of seawater salinity stress.

La salinidad disminuye el rendimiento en zonas áridas y semiáridas. Con el aumento de la demanda de agua de riego, se están buscando fuentes alternativas. El agua de mar se consideró previamente inutilizable para irrigación debido a su salinidad. Sin embargo, esta agua puede ser utilizada con éxito en cultivos bajo ciertas condiciones. Los aminoácidos son bioestimulantes bien conocidos por sus efectos positivos sobre el crecimiento y rendimiento, y por mitigar significativamente las lesiones causadas por estrés abióticos. Por lo tanto, en el presente studio se investigó el efecto del tratamiento exógeno con aminoácidos sobre plantas de haba que crecen bajo estrés salino por irrigación con agua de mar. Se evaluó la reducción de daños por salinidad en plantas de haba mediante el uso de una mezcla de aminoácidos para mejorar los parámetros morfológicos y bioquímicos, y por lo tanto elevar el nivel de rendimiento de la planta. Se desarrolló un experimento en macetas para paliar los efectos nocivos de la salinidad del agua de mar en el haba cv. Giza 843 por aspersion foliar de una mezcla de aminoácidos con diferentes concentraciones (0, 500, 1000 o 1500 mg L-1). El riego de plantas de haba con niveles de agua de mar de 3.13 y 6.25 dS m-1 condujo a reducciones significativas en la altura de planta, número de hojas de la planta, peso fresco y seco de los brotes, y en el contenido foliar de pigmentos fotosintéticos, carbohidratos totales, polisacáridos y ácidos nucleicos (ADN y ARN). La salinidad del agua de mar indujo un mayor contenido de Na+ y Cl-, y una disminución del contenido de K+, K+: Na+, Ca2+, Mg2+ y P3+. El riego de plantas de haba con diferentes niveles de agua de mar redujo el rendimiento de semillas y el peso seco total por planta en comparación con las regadas con agua corriente. Además, el contenido de carbohidratos y proteína total en las semillas disminuyeron con el aumento de los niveles de salinidad del agua de mar. La aplicación de aminoácidos por aspersion foliar increment significativamente todos los parámetros reducidos debido al estrés por agua de mar. Sin embargo, el más alto nivel de aminoácidos (1500 mg L-1) ejerce el máximo efecto en el alivio de los efectos nocivos de estrés por salinidad del agua de mar.

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Abazarian R, Yazdani MR, Khosroyar K, Arvin P. Effects of different levels of salinity on germination of four components of lentil cultivars. Afr J Agric Res. 2011;6(5):2761–2766.

Abd El–Monem AA. Polyamines as modulators of wheat growth, metabolism and reproductive development under high temperature stress. Ph.D. Thesis, Ain Shamas Univ., Cairo, Egypt; 2007.

Abdel Aziz NG, Mazher AAM, Farahat MM. Response of vegetative growth and chemical constituents of Thuja orientalis L. plant to foliar application of different amino acids at Nubaria. J Am Sci. 2010;6(3):295-301.

Abdelhamid MT, Shokr MB, Bekheta MA. Growth, root characteristics, and leaf nutrients accumulation of four faba bean (Vicia faba L.) cultivars differing in their Broomrape tolerance and the Soil properties in relation to salinity. Comm Soil Sci Plant Anal. 2010;41(22):2713–2728. Doi: https://doi.org/10.1080/00103624.2010.518263.

Abdelhamid MT, Sadak MSH, Schmidhalter U, El-Saady A. Interactive effects of salinity stress and nicotinamide on physiological and biochemical parameters of faba bean plant. Acta biol. Colomb. 2013;18(3):499-510.

Abo Sedera FA, Abd El-Latif AA, Bader LAA, Rezk SM. Effect of NPK mineral fertilizer levels and foliar application with humic and amino acids on yield and quality of strawberry. Egypt J Appl Sci. 2010;25:154-169.

Apel K, Hirt H. Reactive oxygen species: Metabolism, oxidative stress and signal transduction. Annu Rev Plant Biol. 2004;55:373–399. Doi: https://doi.org/10.1146/annurev.arplant.55.031903.141701.

Asada K. Ascorbate peroxidase-a hydrogen peroxide-scavenging enzyme in plants. Physiol Plant. 1992;85:235–241. Doi: https://doi.org/10.1111/j.1399-3054.1992.tb04728.x.

Asik BB, Turan MA, Celik H, Katkat AV. Effects of humic substances on plant growth and mineral nutrients uptake of wheat (Triticum durum cv. Salihli) under conditions of salinity. Asian J Crop Sci. 2009;1:87-95. Doi: https://doi.org/10.3923/ajcs.2009.87.95.

Awad, MM, Abd El-Hameed AM, Shall ZS. Effect of glycine, lysine and nitrogen fertilizer rates on growth, yield and chemical composition of potato. J Agric Sci Mansoura Univ. 2007; 32(10):8541-8551.

Awad N, Turky A, Abdelhamid MT, Attia M. Ameliorate of environmental salt stress on the growth of Zea mays L. plants by exopolysaccharides producing bacteria. J Appl Sci Res. 2012;8(4):2033-2044.

Azooz MM. Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes differing in salt tolerance. Int J Agric Biol. 2009;11(4):343-350.

Bekheta MA, Abdelhamid MT, El-Morsi AA. Physiological response of Vicia faba to prohexadione-calcium under saline conditions. Planta Daninha 2009;27(4):769-779. Doi: https://doi.org/10.1590/S0100-83582009000400015.

Boras M, Zidan R, Halloum W. Effect of amino acids on growth, production and quality of tomato in plastic greenhouse. Tishreen Univ. J Res. and Sc Studies. Biolog Sci Series. 2011;33(5):229-238.

Bradford MM. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Biochem. 1976;72:248-54. Doi: https://doi.org/10.1016/0003-2697(76)90527-3.

Burton K. A study of the conditions and mechanism of the diphenylamine reaction of colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956;62(2):315–323.

Cuartero J, Bolarin MC, Asins MJ, Moreno V. Increasing salt tolerance in the tomato. J Exp Bot. 2006;57:1045–1058. Doi: https://doi.org/10.1093/jxb/erj102.

CuinTA, Miller AG, Laurie SA, Leigh RA. Potassium activities in cell compartments of salt-grown barley leaves. J Exp Bot. 2003;54:657–661. Doi: https://doi.org/10.1093/jxb/erg072.

Cvetkovska M, Rampitsch C, Bykova N, Xing T. Genomic analysis of MAP kinase cascades in Arabidopsis defense responses. Plant Mol Biol Rep. 2005;23:331-343. Doi: https://doi.org/10.1007/BF02788882.

Davies DD. Physiological aspects of protein turn over. Encycl Plant Physiol. 1982;45:481–487.

Dawood MG, Abdelhamid MT, Schmidhalter U. Potassium fertiliser enhances the salt-tolerance of common bean (Phaseolus vulgaris L.). J Hort Sci Biotech. 2014a;89(2):185–192.

Dawood MG, Taie HAA, Nassar RMA, Abdelhamid MT, Schmidhalter U. The changes induced in the physiological, biochemical and anatomical structure of Vicia faba by the exogenous application of proline under seawater stress. S Afr J Bot. 2014b;93:54–63. Doi: https://doi.org/10.1016/j.sajb.2014.03.002.

Dische EL. Physiological effects of certain herbicides on rice field weeds. J Amer Chem Soc.1953;22:3014.

El-Lethy SR, Abdelhamid MT, Reda F. Effect of potassium application on wheat (Triticum aestivum L.) cultivars grown under salinity stress. World Appl Sci J. 2013;26(7):840-850. Doi: 10.5829/idosi.wasj.2013.26.07.13527.

El-Zohiri SSM, Asfour YM. Effect of some organic compounds on growth and productivity of some potato cultivars. Annals of Agric Sci Moshtohor. 2009;47(3):403-415.

Erdal S, Aydın M, Genisel M, Taspınar MS, Dumlupinar R, Kaya O, and Gorcek Z. Effects of salicylic acid on wheat salt sensitivity. Afr J Biotechnol. 2011;(30):5713-5718.

Gomez KA, Gomez A. A. Statistical Procedures for Agricultural Research. John Wiley & Sons Inc., Singapore; 1984; p. 680.

Goss, JA. Amino acid synthesis and metabolism. In Physiology of. Plants and their cells. Pergamon Press, Inc., New York; 1973. p.202. Doi: https://doi.org/10.1016/B978-0-08-017036-7.50013-1.

Horie T, Yoshida K, Nakayama H, Yamada K, Oiki S, Shinmyo A. Two types of HKT transporters with different properties of Na+ and K+ transport in Oryza sativa. Plant J. 2001;27:129–138. Doi: https://doi.org/10.1046/j.1365-313x.2001.01077.x.

Hu Y, Schmidhalter U. Drought and salinity: A comparison of their effects on the mineral nutrition of plants. J Plant Nutr Soil Sci. 2005;168:541-549. Doi: https://doi.org/10.1002/jpln.200420516.

Jackson ML. Soil chemical analysis. 1st Edition. Prentice Hall of India Pvt. Ltd., New Delhi, India. 1973; p. 61-73.

Kowalczyk K, Zielony T. Effect of Aminoplant and Asahi on yield and quality of lettuce grown on rockwool. Conf.of biostimulators in modern agriculture, 7-8 Febuary 2008, Warsaw, Poland; 2008.

Lichtenthaler HK, Buschmann C. Chlorophylls and carotenoids: measurement and characterization by UVVIS spectroscopy. In: Wrolstad RE, Acree TE, An H, Decker EA, Penner MH, Reid DS, Schwartz SJ, Shoemaker CF, Sporns P, editors. Current protocols in food analytical chemistry (CPFA). John Wiley and Sons, New York; 2001. p. F4.3.1–F4.3.8.

Liu Xing Q, Chen HY, Qin-xue N, Seung LK. Evaluation of the Role of mixed amino acids in nitrate uptake and assimilationin leafy radish by using 15n-labeled nitrate. Agric Sci CHN. 2008;7(10):1196-1202. Doi: https://doi.org/10.1016/S1671-2927(08)60164-9.

Marschner H. Mineral Nutrition of Higher Plants Gulf Professional Publishing, 1995; p.889.

Morse ML, Carter CE. The synthesis of nucleic acid in cultures of Escherchia coli, strain B and B/R. J Bacteriol. 1949;58(3):317–326.

Morse ML, Carter CE. The synthesis of nucleic acids in cultures of Escherichia coli, strains B and B/R. J Bacteriol. 1949;58:317-326.

Munns R, Tester M. Mechanisms of salinity tolerance. Annu Rev Plant Biol. 2008;59:651–681. Doi: https://doi.org/10.1146/annurev.arplant.59.032607.092911.

Naguib MI. Colourimetric estimation of plant polysaccharides, Zeit-Zucher. 1963;16:15-22.

Neuberg M, Pavlikova D, Pavlik M, Balik J. The effect of different nitrogen nutrition on proline and asparagines content in plant. Plant Soil Environ. 2010;56(7):305–311.

Rai VK. Role of amino acids in plant responses to stress. Biol Plant. 2002;45:471–478. Doi: https://doi.org/10.1023/A:1022308229759.

Raza SH, Athar HR, Ashraf M. Influence of exogenously applied glycine betaine on photosynthetic capacity of differently adapted wheat cultivars under salt stress. Pak J Bot. 2006;38(2):341–351.

Sadak MSh, Abdelhamid MT, El- Saady AM. Physiological responses of Faba Bean Plant to Ascorbic Acid Grown under Salinity Stress. Egypt. J Agron. 2010;32(1):89-106.

Sadak MSh, Abd El-Monem AA, El-Bassiouny HMS, Badr NM. Physiological response of sunflower (Helianthus annuus L.) to exogenous arginine and putrescine treatments under salinity Stress. J Appl Sci Res, 2012;8(10):4943-4957.

Saeed MR, Kheir AM, Al-Sayed AA. Supperssive effect of some amino acids against Meloidogyne incognita on soybeans. J. Agric. Sci. Mansoura Univ. 2005;30(2):1097–1103.

Sairam RK, Tyagi A. Physiology and molecular biology of salinity stress tolerance in plants. Curr Sci. 2004;86:407-412.

Salisbury FB, Ross CW. Sodium accumulation in leaves of Triticum species that differ in salt tolerance. Aust J Plant Physiol 1992;19:331–340. Doi: https://doi.org/10.1071/PP9920331.

Schmidt G, Thannhauser, SJ. A method for the determination of desoxyribonucleic acid, ribonucleic acid, and phosphoproteins in animal tissues. J Biol Chem. 1945;161:83-89.

Serrano R, Rodriguez-Navarro A. Ion homeostasis during salt stress in plants. Curr Opin Cell Biol. 2001;13:399–404. Doi: https://doi.org/10.1016/S0955-0674(00)00227-1.

Souza RP, Machado EC, Silva JAB, Lagôa AMMA, Silveira JAG. Photosynthetic gas exchange, chlorophyll fluorescence and some associated metabolic changes in cowpea (Vigna unguiculata) during water stress and recovery. Environ Exp Bot. 2004;51:45–56. Doi: https://doi.org/10.1016/S0098-8472(03)00059-5.

Taffouo VD, Kouamou JK, Ngalangue LMT, Ndjeudji BAN. Effects of salinity stress on growth, ion partitioning and yield of some cowpea (Vigna unguiculata L. Walp.) cultivars. Int J Bot. 2009;5(2):135-143. Doi: https://doi.org/10.3923/ijb.2009.135.143.

Taie HAA, Abdelhamid MT, Dawood MG, Nassar RMA. Pre-sowing Seed Treatment with Proline Improves some Physiological, Biochemical and Anatomical Attributes of Faba Bean Plants under Sea Water Stress. J Appl Sci Res. 2013;9(4):2853-2867.

Takahashi R, Nishio T, Ichizen N,Takano T. Salt-tolerant reed plants contain lower Na+ and higher K+ than salt-sensitive reed plants. Acta Physiol Plant. 2007;29:431–438. Doi: https://doi.org/10.1007/s11738-007-0052-3.

Tal M. Physiology of polyploidy plants: DNA, RNA, protein and abscisic acid in autotetraploid and diploid tomato under low and high salinity. Bot Gaz. 1977;138:119-1122. Doi: https://doi.org/10.1086/336905.

Thon M, Maretzki A, Korner E, Soki WS. Nutrient uptake and accumulation by sugar cane cell culture in relation to growth cycle. Plant Cell Tiss Org. 1981;(1):3-14.

Treichel S. The effect of NaCl on the concentration of proline in different halophytes. Z. Pflanzen physiol. 1975; 76: 56 68.

Tsenov EI, Strogonov BP, Kabanov VV. Effect of NaCl on the content and synthesis of nucleic acid in tomato tissues. Fiziol Rast. 1973;20:54-61.

Walter GR, Nawacki E. Alkaloid biolog and metabolism in plants. Planum, press, N.Y; 1978. p.152.

Wenxue W, Bilsborrow PE, Hooley P, Fincham DA, Lombi E, Forster BP. Salinity induced differences in growth, ion distribution and partitioning in barley between the cultivar Maythorpe and its derived mutant Golden Promise. Plant Soil. 2003;250:183–191. Doi: https://doi.org/10.1023/A:1022832107999.

Zeid IM. Alleviation of Seawater Stress during Germination and Early Growth of Barley. Int J Agric Res Rev. 2011;(2):59-67.

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