GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS

Vanessa Lewandowski; Cesar Sary; Jaisa Casetta; Felipe Pinheiro de Souza; Pedro Luiz de Castro; Elenice Souza dos Reis Goes; Carlos Antonio Lopes de Oliveira; Ricardo Pereira Ribeiro; Lauro Vargas

doi:10.15446/abc.v27n2.87739

Publicado

2021-12-15

GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS

Variabilidad genética y características reproductivas de poblaciones de pez cebra (Cyprinidae Danio rerio)

DOI:

https://doi.org/10.15446/abc.v27n2.87739

Palabras clave:

Animal model, consanguinity, DNA, female, fish larvae (en)
Modelo animal, consanguinidad, ADN, hembra, larvas de peces (es)

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Autores/as

Vanessa Lewandowski Universidad Federal de Grande Dourados https://orcid.org/0000-0003-4946-5805
Cesar Sary Universidad Estatal de Maringá https://orcid.org/0000-0001-5342-8105
Jaisa Casetta Universidad Estatal de Maringá https://orcid.org/0000-0001-9994-077X
Felipe Pinheiro de Souza Universidad Estatal de Londrina https://orcid.org/0000-0003-3436-4147
Pedro Luiz de Castro Universidad Estatal de Mato Grosso do Sul https://orcid.org/0000-0001-7351-6938
Elenice Souza dos Reis Goes Universidad Federal de Grande Dourados https://orcid.org/0000-0003-2437-4800
Carlos Antonio Lopes de Oliveira Universidad Estatal de Maringá https://orcid.org/0000-0003-2504-3261
Ricardo Pereira Ribeiro Universidad Estatal de Maringá https://orcid.org/0000-0001-7752-3692
Lauro Vargas Universidad Estatal de Maringá https://orcid.org/0000-0002-1221-7384

Resumen (en)
Resumen (es)

Specimens of cultured zebrafish acquired from different fish farms in Brazil may show genetic variability and alteration in allele frequency due to genetic drift and selective pressure in a captive environment, resulting in the differentiation of productive and reproductive characteristics. The aim of this study was to evaluate the genetic variability and reproductive characteristics of 180 zebrafish specimens from six Brazilian fish farms. A deviation from the Hardy-Weinberg equilibrium was observed in all evaluated stocks. Differentiation among stocks was observed in the amount of genetic variability with respect to observed heterozygosity and the inbreeding coefficient (F_IS). Genetic distance between stocks was determined through the F_st index, and the formation of four distinct groups was observed by plotting the dendrogram based on Nei’s genetic distance. Differences were observed among reproductive parameters, such as the average number of eggs per female and hatchability. This second parameter proved to be related to the level of inbreeding of the population, whereas this effect was not observed for spawning frequency. We conclude that zebrafish stocks from the 6 different Brazilian fish farms present significant genetic and phenotypic variability. The genetic structure affects fecundity and should be considered when carrying out work where reproductive rates are evaluated.

Especímenes de pez cebra adquiridos en diferentes piscifactorías pueden mostrar variabilidad genética y alteración en la frecuencia de los alelos debido a la deriva genética y presión selectiva llevada a cabo en un ambiente cautivo, lo que resulta en la diferenciación de las características productivas y reproductivas. Este estudio busco evaluar la variabilidad genética y las características reproductivas de 180 especímenes de pez cebra adquiridos de seis piscifactorías brasileras. Hubo una desviación en el equilibrio de Hardy-Weinberg en todas las poblaciones evaluadas. Se encontró diferenciación en términos del grado de variabilidad dentro de las poblaciones, en vista de los resultados de la heterocigosidad observada y el coeficiente de endogamia (F_is). La distancia genética entre ellos se verificó usando el índice F_st, y se observó la formación de cuatro grupos distintos al trazar el dendrograma basado en la distancia genética de Nei. Se observó una diferencia en relación con los parámetros reproductivos, como el número promedio de huevos por hembra y la incubabilidad. Este segundo parámetro demostró estar relacionado con el nivel de consanguinidad de la población, y este efecto no se verificó para la frecuencia de desove. Se puede considerar que las existencias de pez cebras de diferentes lugares tienen variabilidad genética y fenotípica. La estructura genética influye principalmente en la fertilización y debe tenerse en cuenta al realizar trabajos donde se evalúan los índices reproductivos.

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APA

Lewandowski, V., Sary, C., Casetta, J., de Souza, F. P., de Castro, P. L., Goes, E. S. dos R., de Oliveira, C. A. L., Ribeiro, R. P. y Vargas, L. (2021). GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS. Acta Biológica Colombiana, 27(2), 223 231. https://doi.org/10.15446/abc.v27n2.87739

ACM

[1]

Lewandowski, V., Sary, C., Casetta, J., de Souza, F.P., de Castro, P.L., Goes, E.S. dos R., de Oliveira, C.A.L., Ribeiro, R.P. y Vargas, L. 2021. GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS. Acta Biológica Colombiana. 27, 2 (dic. 2021), 223 231. DOI:https://doi.org/10.15446/abc.v27n2.87739.

ACS

(1)

Lewandowski, V.; Sary, C.; Casetta, J.; de Souza, F. P.; de Castro, P. L.; Goes, E. S. dos R.; de Oliveira, C. A. L.; Ribeiro, R. P.; Vargas, L. GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS. Acta biol. Colomb. 2021, 27, 223 231.

ABNT

LEWANDOWSKI, V.; SARY, C.; CASETTA, J.; DE SOUZA, F. P.; DE CASTRO, P. L.; GOES, E. S. dos R.; DE OLIVEIRA, C. A. L.; RIBEIRO, R. P.; VARGAS, L. GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS. Acta Biológica Colombiana, [S. l.], v. 27, n. 2, p. 223 231, 2021. DOI: 10.15446/abc.v27n2.87739. Disponível em: https://revistas.unal.edu.co/index.php/actabiol/article/view/87739. Acesso em: 8 ene. 2025.

Chicago

Lewandowski, Vanessa, Cesar Sary, Jaisa Casetta, Felipe Pinheiro de Souza, Pedro Luiz de Castro, Elenice Souza dos Reis Goes, Carlos Antonio Lopes de Oliveira, Ricardo Pereira Ribeiro, y Lauro Vargas. 2021. «GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS». Acta Biológica Colombiana 27 (2):223 231. https://doi.org/10.15446/abc.v27n2.87739.

Harvard

Lewandowski, V., Sary, C., Casetta, J., de Souza, F. P., de Castro, P. L., Goes, E. S. dos R., de Oliveira, C. A. L., Ribeiro, R. P. y Vargas, L. (2021) «GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS», Acta Biológica Colombiana, 27(2), p. 223 231. doi: 10.15446/abc.v27n2.87739.

IEEE

[1]

V. Lewandowski, «GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS», Acta biol. Colomb., vol. 27, n.º 2, p. 223 231, dic. 2021.

MLA

Lewandowski, V., C. Sary, J. Casetta, F. P. de Souza, P. L. de Castro, E. S. dos R. Goes, C. A. L. de Oliveira, R. P. Ribeiro, y L. Vargas. «GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS». Acta Biológica Colombiana, vol. 27, n.º 2, diciembre de 2021, p. 223 231, doi:10.15446/abc.v27n2.87739.

Turabian

Lewandowski, Vanessa, Cesar Sary, Jaisa Casetta, Felipe Pinheiro de Souza, Pedro Luiz de Castro, Elenice Souza dos Reis Goes, Carlos Antonio Lopes de Oliveira, Ricardo Pereira Ribeiro, y Lauro Vargas. «GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS». Acta Biológica Colombiana 27, no. 2 (diciembre 15, 2021): 223 231. Accedido enero 8, 2025. https://revistas.unal.edu.co/index.php/actabiol/article/view/87739.

Vancouver

1.

Lewandowski V, Sary C, Casetta J, de Souza FP, de Castro PL, Goes ES dos R, de Oliveira CAL, Ribeiro RP, Vargas L. GENETIC VARIABILITY AND REPRODUCTIVE CHARACTERISTICS OF ZEBRAFISH (Cyprinidae Danio rerio) STOCKS. Acta biol. Colomb. [Internet]. 15 de diciembre de 2021 [citado 8 de enero de 2025];27(2):223 231. Disponible en: https://revistas.unal.edu.co/index.php/actabiol/article/view/87739

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