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VARIACIÓN EN LA EXPRESIÓN DE GENES DE Apis mellifera L. (Hymenoptera: Apidae) POR EXPOSICIÓN A UNA DOSIS SUBLETAL DE FLUPIRADIFURONA
Variation in Apis mellifera L. (Hymenoptera: Apidae) gene expression by exposure to a sublethal dose of Flupyradifurone
DOI:
https://doi.org/10.15446/abc.v28n3.92972Palabras clave:
agonistas del receptor nicotínico de acetilcolina, efecto subletal, estrés oxidativo, flupiradifurona, disminución de polinizadores (es)nicotinic acetylcholine receptor agonist, sublethal effect, oxidative stress, flupyradifurone, pollinators decline (en)
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La industria de los insecticidas ha desarrollado ingredientes activos más seguros para las abejas como la flupiradifurona. Sin embargo, investigaciones recientes proporcionan evidencia de los efectos negativos de esta y su posible influencia en el deterioro de la salud de los polinizadores. Durante el experimento, abejas obreras de edades mixtas de A. mellifera fueron expuestas a una dosis oral, única y subletal de flupiradifurona (645 ng/abeja) y se evaluaron solo durante el primer día tras la exposición a 0,5, 1, 3, 6 y 24 horas. Mediante la reacción en cadena de la polimerasa con transcriptasa inversa (RT-qPCR, siglas en inglés), se cuantificó la alteración de la expresión génica relativa ocasionada tras la exposición a este insecticida. Un total de cinco genes fueron cuantificados: tres genes antioxidantes primarios (Catalasa (CAT), Superóxido dismutasa (SOD1) y Tioredoxin dismutasa (Trxr1), un gen de detoxificación (Citocromo P450 9Q3 (CYP9Q3)) y un gen con actividad neuronal (Acetilcolinesterasa, AChE1). El gen endógeno proteina ribosómica S18 (RPS18) permitió estimar la expresión génica relativa como la respuesta a la exposición al insecticida. El gen AChE1 fue sobreexpresado hasta las seis horas tras la exposición; los restantes genes CYP9Q3, CAT, SOD1 y Trxr1 presentaron un comportamiento similar. En una dosis subletal y a intervalos de tiempo corto, la flupiradifurona causa alteración en la expresión relativa de cinco genes en individuos de abejas melíferas tras la exposición oral, lo cual podría causar a largo plazo un efecto adverso en la salud de las colmenas.
The insecticide industry has developed more bee-safe active ingredients such as flupyradifurone. However, recent research provides evidence of the negative effects of this and its possible influence on the deterioration of the health of pollinators. During the experiment, mixed-age worker bees of A. mellifera were exposed to a single, sublethal oral dose of flupyradifurone (645 ng/ bee) and were assessed only for the first day after exposure to 0.5, 1, 3, 6, and 24 hours. Using reverse transcriptase polymerase chain reaction (RT-qPCR), the alteration in gene expression caused y exposure to this insecticide was quantified. A total of five genes were quantified: three primary antioxidant genes (Catalase (CAT), Superoxide dismutase (SOD1) and Thioredoxin dismutase (Trxr1), one detoxification gene (Cytochrome P450 9Q3 (CYP9Q3)) and one gene with neuronal activity (Acetylcholinesterase, AChE1). The endogenous ribosomal protein S18 (RPS18) gene allowed the estimation of relative gene expression as the response to insecticide exposure. The AChE1 gene was overexpressed up to six hours after exposure; the remaining CYP9Q3, CAT, SOD1, and Trxr1 genes presented a similar behavior. At a sublethal dose and at short time intervals, flupyradifurone causes alteration in the relative expression of five genes in individual honeybees after oral exposure, which could cause a long-term adverse effect on hive health.
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