Publicado
BIOLOGICAL ACTIVITIES AND CHEMICAL COMPOSITION OF Rubia tinctorum (L) ROOT AND AERIAL PART EXTRACTS THEREOF
Actividades biológicas y composición química de los extractos de raíz y parte aérea de Rubia tinctorum L.
DOI:
https://doi.org/10.15446/abc.v27n3.95476Palabras clave:
bioactivity, chemical composition, free radical, Rubia tinctorum L. (en)bioactividad, composición química, radical libre, Rubia tinctorum L (es)
Descargas
In the present study, the phytochemical composition, antioxidant and anti-hemolytic activities of root and aerial part Rubia tinctorum hydromethanolic extracts were investigated. Phytochemical screening reveals the presence of phenol, alkaloids, coumarin, flavonoids, and tannins in both extracts. LC-ESI-MS/MS analysis showed the presence of 15 and 17 components in the root and the aerial part extracts, respectively. The quantitative analysis indicates the richness of root extracts in phenolic compounds (118.38 mg GAE/g) and flavonoids (45.29±0.04 mg GAE/g), the aerial part extract has the highest levels in tannins (134.1±0.1 mg GAE/g). Besides, aerial part extracts revealed the highest antioxidant activity for DPPH (83.23 %) and FRAP (1.51±0.22), while the root extract exhibited the highest potential for TAC and antihemolytic activity (61.09 %). Rubia tinctorum contains different active compounds to prevent diseases related to oxidative stress.
En el presente estudio se investigó la composición fitoquímica y las actividades antioxidante, y antihemolítica de los extractos hidrometanólicos de la raíz y la parte aérea de R. tinctorum. El cribado fitoquímico revela la presencia de fenol, alcaloides, cumarina, flavonoides y taninos en ambos extractos. El análisis LC-ESI-MS/MS mostró la presencia de 15 y 17 componentes en los extractos de la raíz y la parte aérea, respectivamente. El análisis cuantitativo indica la riqueza de los extractos de la raíz en compuestos fenólicos (118, 38 mg GAE/g) y flavonoides (45, 29±0, 04 mg GAE/g), el extracto de la parte aérea tiene los niveles más altos en taninos (134, 1±0, 1 mg GAE/g). Además, los extractos de la parte aérea revelaron la mayor actividad antioxidante para DPPH (83, 23 %) y FRAP (1, 51 ±0, 22), mientras que el extracto de la raíz exhibió el mayor potencial para TAC y actividad antihemolítica (61,09 %). Rubia tinctorum contiene diferentes compuestos activos para prevenir enfermedades relacionadas con el estrés oxidativo.
Referencias
Abodunrin, T., Uhuegbu, C., and Olugbuyiro, J (2015). Phytochemical analysis of leaf-extracts from eight tropical trees: Prospects for environmentally-friendly dye compounds for smart windows. International Journal of Scientific and Engineering Research, 6(3), 682-698.
Aboud, A. S.(2010). HPLC analysis of Rubia tinctorum and its effect of methanol and aqueous extract on bacteria isolated from burns infection. Al-Nahrain Journal of Science, 13(4), 166-175. https://doi.org/10.22401/JNUS.13.4.22
Adou, L. M. D., Kone, M. W., Ipou, J. I., and N’guessan, E. K. (2016). Ethnobotanique et analyse phytochimique qualitative de Pteridium aquilinum (L.) Kühn (Dennstaedtiaceae), une Ptéridophyte utilisée comme plante médicinale en Côte d’Ivoire. International Journal of Biological and Chemical Sciences, 10(4), 1783-1792. https://doi.org/10.4314/ijbcs.v10i4.27
Afsar, T., Razak, S., Khan, MR., Mawash, S., Almajwal, A., Shabir,M., and Ul Haq, I.(2016). Evaluation of antioxidant, anti-hemolytic and anticancer activity of various solvent extracts of Acacia hydaspica R. Parker aerial parts. BMC complementary and alternative medicine, 16(1), 258.https://doi.org/10.1186/s12906-016-1240-8.
Amezouar, F., Badri, W., Hsaine, M., Bourhim, N., and Fougrach, H.(2013). Antioxidant and anti-inflammatory activities of Moroccan Erica arborea L. Pathologie- Biologie, 61(6), 254-258.https://doi.org/10.1016/j.patbio.2013.03.005
Aye, MM., Aung, HT., Sein, MM., and Armijos, C. (2019). A review on the phytochemistry, medicinal properties and pharmacological activities of 15 selected Myanmar medicinal plants. Molecules, 24(2), 293.https://doi.org/10.3390/molecules24020293
Azab, A., Nassar, A., and Azab, A. N. (2016). Antiinflammatory activity of natural products. Molecules, 21(10), 1321. https://doi.org/10.3390/molecules21101321
Bouayed. J., Rammal, H., Dicko, A., Younos, C., and Soulimani, R. (2007). Chlorogenic acid, a polyphenol from Prunus domestica (Mirabelle), with coupled anxiolytic and antioxidant effects. Journal of the neurological sciences, 262(1-2), 77-84. https://doi.org/10.1016/j.jns.2007.06.028
Carvalho, L. V. D. N., Cordeiro, M. F., Sampaio, M. C. P. D., De Mello, G. S. V., Da Costa, V. D. C. M., Marques, L. L. M., Klein, T., Palazzo de Mello, J. C., Ferro Cavalcanti, I. M., da Rocha Pitta, I., da Rocha Pitta, M. G., and M. J. Barreto de Melo Rêgo. (2016). Evaluation of antibacterial, antineoplastic, and immunomodulatory activity of Paullinia cupana seeds crude extract and ethyl-acetate fraction. Evidence-Based Complementary and Alternative Medicine. 2016, 1203274.https://doi.org/10.1155/2016/1203274
Cemeli, E., Baumgartner, A., and Anderson, D.(2009). Antioxidants and the Comet assay. Mutation Research/ Reviews in Mutation Research,681(1), 51-67.https://doi.org/10.1016/j.mrrev.2008.05.002
Cezarotto, V. S., Giacomelli, S. R., Vendruscolo, M. H., Vestena, A. S., Cezarotto, C. S., Da cruz, R. C., Maurer, L. H., Ferreira, L. M., Emanuelli, T., and Cruz, L.(2017). Influence of harvest season and cultivar on the variation of phenolic compounds composition and antioxidant properties in Vaccinium ashei leaves. Molecules, 22(10):1603. https://doi.org/10.3390/molecules22101603PMid:28973959
Chaudhuri, S., Banerjee, A., Basu, K., Sengupta, B., and Sengupta, PK. 2007. Interaction of flavonoids with red blood cell membrane lipids and proteins: antioxidant and antihemolytic effects. International journal of biological macromolecules, 41(1), 42-48. https://doi.org/10.1016/j.ijbiomac.2006.12.003
Derksen, G. C., Niederländer, H. A., and Van beek, T. A. (2002). Analysis of anthraquinones in Rubia tinctorum L. by liquid chromatography coupled with diode-array UV and mass spectrometric detection. Journal of chromatography A, 978(1-2), 119-127. https://doi.org/10.1016/S0021-9673(02)01412-7
Eltamany, E. E., Nafie, M. S., Khodeer, D. M., EL-tanahy, A. H., Abdel-kader, M. S., Badr, J. M, and Abdelhameed, R. F. A. (2020). Rubia tinctorum root extracts: chemical profile and management of type II diabetes mellitus. RSC Advances, 10(41), 24159-24168. https://doi.org/10.1039/D0RA03442H
Essaidi, I., Snoussi, A., Koubaier,H. B. H., Casabianca, H., and Bouzouita, N.(2017). Effect of acid hydrolysis on alizarin content, antioxidant and antimicrobial activities of Rubia tinctorum extracts. Pigment and Resin Technology, 46(5), 379-384. https://doi.org/10.1108/PRT-11-2015-0116
Haddouchi, F., Chaouche, T., and Halla, N. 2016. Screening phytochimique, activités antioxydantes et pouvoir hémolytique de quatre plantes sahariennes d’Algérie. Phytothérapie, 1-9. https://doi.org/10.1007/s10298-016-1086-8
Hamdi, A., Viane, J., Mahjoub, M. A., Majouli, K., Gad, M. H. H., Kharbach, M., Demeyer, K., Marzouk, Z., and Heydenaet, Y. V.(2018). Polyphenolic contents, antioxidant activities and UPLC-ESI-MS analysis of Haplophyllum tuberculatum A. Juss leaves extracts. International journal of biological macromolecules, 106, 1071-1079. https://doi.org/10.1016/j.ijbiomac.2017.08.107PMid:28851641
Henderson, R. L. (2013). The chemical profile of Rubia tinctorum in wool dyeing and a novel fibre extraction method for compositional analysis. University of Leeds.
Hossain, MA., Al-raqmi, K. A. S., AL-mijizy, Z. H., Weli,A. M., and AL-Riyami,Q. (2013). Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris. Asian Pacific journal of tropical biomedicine, 3(9), 705-710. https://doi.org/10.1016/S2221-1691(13)60142-2
Idris, O. A., Wintola, O. A., and Afolayan, A. J.(2017). Phytochemical and antioxidant activities of Rumex crispus L. In treatment of gastrointestinal helminths in Eastern Cape Province, South Africa. Asian Pacific journal of tropical biomedicine, 7(12), 1071-1078. https://doi.org/10.1016/j.apjtb.2017.10.008
Iloki-assanga, S. B., Lewis-luján, L. M., Lara-espinoza, C. L., Gil-salido, A. A., Fernandez-angulo, D., Rubio-pino,J. L.,and Haines, D. D. (2015). Solvent effects on phytochemical constituent profiles and antioxidant activities, using four different extraction formulations for analysis of Bucida buceras L. and Phoradendron californicum. BMC research notes, 8(1), 1-14. https://doi.org/10.1186/s13104-015-1388-1PMid:26323940PMCid:PMC4553924
In,Y. W., Kim, J. J., Kim, H. J., and Oh, S. W. (2013). Antimicrobial activities of acetic acid, citric acid and lactic acid against S higella species. Journal of Food Safety, 33(1), 79-85.https://doi.org/10.1111/jfs.12025
Ismail, A. M., Mohamed, E. A., Marghany, M. R., Abdelmotaal, F. F., Abdel-farid, I. B., and EL-sayed, M. A. (2016). Preliminary phytochemical screening, plant growth inhibition and antimicrobial activity studies of Faidherbia albida legume extracts. Journal of the Saudi Society of Agricultural Sciences, 15(2), 112-117.https://doi.org/10.1016/j.jssas.2014.06.002
Joshi, A., Bhobe, M., and Sattarkar, A.(2013). Phytochemical investigation of the roots ofGrewia microcosLinn. Journal of Chemical and Pharmaceutical Research, 5(7), 80-87.
Kadam, D., Palamthodi,S., and Lele, S. (2018). LC-ESIQ-TOF-MS/MS profiling and antioxidant activity of phenolics from L. Sativum seedcake. Journal of food science and technology, 55(3), 1154-1163.https://doi.org/10.1007/s13197-017-3031-8PMid:29487458PMCid:PMC5821675
Khan, R. A., Khan, M. R., Sahreen, S., and Ahmed, M.(2012). Assessment of flavonoids contents and in vitro antioxidant activity of Launaea procumbens. Chemistry Central Journal, 6(1), 1-11. https://doi.org/10.1186/1752-153X-6-43PMid:22616896PMCid:PMC3542575
Köksal, E., Tohma, H., KıLıÇ, Ö., Alan, Y., Aras,A., Gülçin.İ., and Bursal, E.(2017). Assessment of antimicrobial and antioxidant activities of Nepeta trachonitica: analysis of its phenolic compounds using HPLC-MS/MS. Scientia pharmaceutica, 85(2),24. https://doi.org/10.3390/scipharm85020024
Kumar Bargah, R. (2015). Preliminary test of phytochemical screening of crude ethanolic and aqueous extract of Moringa pterygosperma Gaertn. Journal of Pharmacognosy and Phytochemistry, 4(1).
Lajkó, E., Bányai, P., Zámbó, Z., Kursinszki, L., Szőke, É., and Kőhidai, L. (2015). Targeted tumor therapy by Rubia tinctorum L.: analytical characterization of hydroxyanthraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines (A2058 and HT168-M1). Cancer cell international, 15(1), 1-15. https://doi.org/10.1186/s12935-015-0271-4PMid:26690297PMCid:PMC4683936
Langa-lomba, N., Sánchez-hernández, E., Buzón-durán, L., González-garcía, V., Casanova-Gascón, J., Martín-gil, J.|,and Martín-Ramos, P.(2021). Activity of anthracenediones and flavoring phenols in hydromethanolic extracts of Rubia tinctorum against grapevine phytopathogenic fungi. Plants, 10(8), 1527. https://doi.org/10.3390/plants10081527PMid:34451572PMCid:PMC8399478
Lotfollahi, P., Irani-nejad, K. H., Heydari Rikan, M., and De lillo, E. (2014). Two new eriophyid mites (Acari: Trombidiformes: Eriophyoidea) on Rubia tinctorum L. in Iran. International Journal of Acarology, 40(3), 225-229. https://doi.org/10.1080/01647954.2014.904436
Louli, V., Ragoussis, N., and Magoulas, K. (2004). Recovery of phenolic antioxidants from wine industry by-products. Bioresource technology, 92(2), 201-208. https://doi.org/10.1016/j.biortech.2003.06.002PMid:14693454
Marhoume, F. Z., Laaradia, M. A., Zaid, Y., Laadraoui, J., Oufquir, S., Aboufatima, R., Chait, A., and Bagria, A.(2019). Anti-aggregant effect of butanolic extract of Rubia tinctorum L on platelets in vitro and ex vivo. Journal of Ethnopharmacology, 241. https://doi.org/10.1016/j.jep.2019.111971
Maria, R., Shirley, M., Xavier, C., Jaime, S., David, V., Rosa, S.,and Jodiea, D. (2018). Preliminary phytochemical screening, total phenolic content and antibacterial activity of thirteen native species from Guayas province Ecuador. Journal of King Saud University-Science, 30(4), 500-505. https://doi.org/10.1016/j.jksus.2017.03.009
Marković, Z. S., Manojlović, N. T., Jeremić, S. R., and Živić, M. (2013). HPLC, UV-vis and NMR spectroscopic and DFT characterization of purpurin isolated from Rubia tinctorum L. Hemijska industrija, 67(1), 77-88. https://doi.org/10.2298/HEMIND120419058M
Ohikhena, F. U., Wintola, O. A., and Afolayan, A. J. (2018). Quantitative phytochemical constituents and antioxidant activities of the mistletoe, phragmanthera capitata (sprengel) balle extracted with different solvents. Pharmacognosy Research, 10(1), 16.
Pharmacognosie, B. J. (1999). Phytochimie, plantes médicinales. Revue et Augmentée, Tec and Doc, Paris.
Prieto, P., Pineda, M., Aguilar, M.(1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical Biochemistry, 269337-341
Rached, W., Barros, L., Ziani,BE., Bennaceur, M., Calhelha, RC., Heleno, SA., Alves, M. J., Marouff, A., and Ferreira, I. C. F. R. (2019). HPLC-DAD-ESI-MS/MS screening of phytochemical compounds and the bioactive properties of different plant parts of Zizyphus lotus (L.) Food and Function journal, 10(9), 5898-5909.https://doi.org/10.1039/C9FO01423CPMid:31465056
Rashid, S., Ahmad, M., Zafar, M., Anwar, A., Sultana, S., Tabassum, S., and Ahmeda, S. N. (2016). Ethnopharmacological evaluation and antioxidant activity of some important herbs used in traditional medicines. Journal of Traditional Chinese Medicine, 36(5), 689-694. https://doi.org/10.1016/S0254-6272(16)30091-7
Rovčanin, BR., Steševi, D., Keki, D., and Risti, M. 2015. Antibacterial effect of Herniaria hirsuta, Prunus avium, Rubia tinctorum and Sempervivum tectorum plant extracts on multiple antibiotic resistant Escherichia coli. Bioscience Journal, 31(6), 1852-1861.https://doi.org/10.14393/BJv31n6a2015-29091
Santos, U. P., Campos, J. F., Torquato, H. F. V., Paredesgamero, E. J., Carollo, C. A., Estevinho, L. M., Souza, K. P., and dos Santos, E. L. (2016). Antioxidant, antimicrobial and cytotoxic properties as well as the phenolic content of the extract from Hancornia speciosa Gomes. PLoS One, 11(12), e0167531.https://doi.org/10.1371/journal.pone.0167531
Savadi, S., Vazifedoost, M., Didar, Z., Nematshahi, MM., and Jahed, E. 2020. Phytochemical analysis and antimicrobial/antioxidant activity of Cynodon dactylon (L.) Pers. rhizome methanolic extract. Journal of Food Quality, 2020.https://doi.org/10.1155/2020/5946541
Shahidi, F., and Ambigaipalan, P. 2015. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects-A review. Journal of functional foods, 18, 820-897. https://doi.org/10.1016/j.jff.2015.06.018
Sharififar, F., Dehghn-nudeh, G., and Mirtajaldini, M. 2009. Major flavonoids with antioxidant activity from Teucrium polium L. Food Chemistry, 112(4), 885-888.https://doi.org/10.1016/j.foodchem.2008.06.064
Siva, R., Palackan, MG., Maimoon, L., Geetha, T., Bhakta, D., Balamurugan, P., and Rajanarayanan, S. 2011. Evaluation of antibacterial, antifungal, and antioxidant properties of some food dyes. Food Science and Biotechnology, 20(1), 7-13. https://doi.org/10.1007/s10068-011-0002-0
Szakiel, A., Pączkowski, C., and Henry, M. 2011. Influence of environmental abiotic factors on the content of saponins in plants. Phytochemistry Reviews, 10(4), 471-491.https://doi.org/10.1007/s11101-010-9177-x
Vadivel, V., and Biesalski,H. K. (2011).Contribution of phenolic compounds to the antioxidant potential and type II diabetes related enzyme inhibition properties of Pongamia pinnata L. Pierre seeds. Process Biochemistry, 46(10), 1973-1980.https://doi.org/10.1016/j.procbio.2011.07.007
Yen, G. -C., and Wu, J. -Y. (1999). Antioxidant and radical scavenging properties of extracts from Ganoderma tsugae. Food Chemistry, 65(3), 375-379.https://doi.org/10.1016/S0308-8146(98)00239-8
Cómo citar
APA
ACM
ACS
ABNT
Chicago
Harvard
IEEE
MLA
Turabian
Vancouver
Descargar cita
CrossRef Cited-by
1. Meysam Soleymani Najafabadi, Amir Shakerian, Zohreh Mashak, Ebrahim Rahimi, Charalampos Proestos. (2024). Production of Smart Packaging Chitosan Films Utilizing Malva sylvestris Mucilage and Rubia tinctorum Extract for Enhancing Fish Freshness and Prolonging Shelf Life. Journal of Food Biochemistry, 2024(1) https://doi.org/10.1155/2024/7275427.
2. Ramazan Erenler, Ibrahim Hosaflioglu. (2023). Green synthesis of silver nanoparticles using Onobrychis sativa L.: Characterization, catalytic degradation of methylene blue, antioxidant activity, and quantitative analysis of bioactive compounds. Materials Today Communications, 35, p.105863. https://doi.org/10.1016/j.mtcomm.2023.105863.
3. Ramazan Erenler, Esma Nur Gecer, Ibrahim Hosaflioglu, Lutfi Behcet. (2023). Green synthesis of silver nanoparticles using Stachys spectabilis: Identification, catalytic degradation, and antioxidant activity. Biochemical and Biophysical Research Communications, 659, p.91. https://doi.org/10.1016/j.bbrc.2023.04.015.
4. Esma Nur Gecer, Ramazan Erenler. (2023). Biogenic synthesis of silver nanoparticles using Echium vulgare: Characterisation, quantitative analysis of bioactive compounds, antioxidant activity and catalytic degradation. Journal of the Indian Chemical Society, 100(5), p.101003. https://doi.org/10.1016/j.jics.2023.101003.
Dimensions
PlumX
Visitas a la página del resumen del artículo
Descargas
Licencia
Esta obra está bajo una licencia internacional Creative Commons Atribución-NoComercial-CompartirIgual 4.0.
1. La aceptación de manuscritos por parte de la revista implicará, además de su edición electrónica de acceso abierto bajo licencia Attribution-NonCommercial-ShareAlike 4.0 (CC BY NC SA), la inclusión y difusión del texto completo a través del repositorio institucional de la Universidad Nacional de Colombia y en todas aquellas bases de datos especializadas que el editor considere adecuadas para su indización con miras a incrementar la visibilidad de la revista.
2. Acta Biológica Colombiana permite a los autores archivar, descargar y compartir, la versión final publicada, así como las versiones pre-print y post-print incluyendo un encabezado con la referencia bibliográfica del articulo publicado.
3. Los autores/as podrán adoptar otros acuerdos de licencia no exclusiva de distribución de la versión de la obra publicada (p. ej.: depositarla en un archivo telemático institucional o publicarla en un volumen monográfico) siempre que se indique la publicación inicial en esta revista.
4. Se permite y recomienda a los autores/as difundir su obra a través de Internet (p. ej.: en archivos institucionales, en su página web o en redes sociales cientificas como Academia, Researchgate; Mendelay) lo cual puede producir intercambios interesantes y aumentar las citas de la obra publicada. (Véase El efecto del acceso abierto).