Publicado

2024-01-22

THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE

Los efectos dependientes de la dosis de cafeína y betaína sobre la actividad de la enzima telomerasa en ratones

DOI:

https://doi.org/10.15446/abc.v29n2.99155

Palabras clave:

Betaine, Caffeine, Catalase, SOD, Telomerase (en)
Betaína, Cafeína, Catalasa, SOD, Telomerasa (es)

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Autores/as

The telomerase enzyme, which extends the Deoxyribonucleic Acid (DNA) regions called telomeres at the ends of the chromosomes, has an important place in aging, cancer and stem cell studies. In this study, the effects of betaine and caffeine on telomerase enzyme activity in mice were investigated. Telomerase activity was measured by a Polymerase Chain Reaction-Enzyme Linked Immunosorbent Assay (PCR-ELISA) based method. The activity of Superoxide Dismutase (SOD) and Catalase enzymes in the liver and kidney and the amount of Malondialdehyde (MDA) were also investigated. The results show that betaine has a slightly inhibitory effect (not significant) on telomerase activity, especially at high doses. Caffeine may act as an inhibitor in high doses but may have an activator effect at low doses. Also, it was observed that SOD and Catalase enzyme activities were parallel to the increase/decrease in telomerase enzyme activity in the liver.

La enzima telomerasa, que extiende las regiones del ácido desoxirribonucleico (ADN) llamadas telómeros en los extremos de los cromosomas, ocupa un lugar importante en los estudios sobre el envejecimiento, el cáncer y las células madre. En este estudio, se investigaron los efectos de la betaína y la cafeína sobre la actividad de la enzima telomerasa en ratones. La actividad de la telomerasa se midió mediante un método basado en PCR-ELISA. También se investigó la actividad de las enzimas SOD y catalasa en hígado y riñón y la cantidad de MDA. Los resultados muestran que la betaína tiene un efecto levemente inhibidor (no significativo) sobre la actividad de la telomerasa, especialmente a dosis altas. La cafeína puede actuar como inhibidor en dosis altas, pero puede tener un efecto activador en dosis bajas. Se observó que las actividades de la enzima SOD y catalasa eran paralelas al aumento / disminución de la actividad de la enzima telomerasa en el hígado.

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Cómo citar

APA

Tikirdik, M., Mutlu, A. G., Alkan, I., Yildiz, H., Korkmaz, D. y Topal, A. (2023). THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE. Acta Biológica Colombiana, 29(2), 5–11. https://doi.org/10.15446/abc.v29n2.99155

ACM

[1]
Tikirdik, M., Mutlu, A.G., Alkan, I., Yildiz, H., Korkmaz, D. y Topal, A. 2023. THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE. Acta Biológica Colombiana. 29, 2 (dic. 2023), 5–11. DOI:https://doi.org/10.15446/abc.v29n2.99155.

ACS

(1)
Tikirdik, M.; Mutlu, A. G.; Alkan, I.; Yildiz, H.; Korkmaz, D.; Topal, A. THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE. Acta biol. Colomb. 2023, 29, 5-11.

ABNT

TIKIRDIK, M.; MUTLU, A. G.; ALKAN, I.; YILDIZ, H.; KORKMAZ, D.; TOPAL, A. THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE. Acta Biológica Colombiana, [S. l.], v. 29, n. 2, p. 5–11, 2023. DOI: 10.15446/abc.v29n2.99155. Disponível em: https://revistas.unal.edu.co/index.php/actabiol/article/view/99155. Acesso em: 6 mar. 2025.

Chicago

Tikirdik, Muazzez, Ayse Gul Mutlu, Irem Alkan, Hulya Yildiz, Didem Korkmaz, y Aykut Topal. 2023. «THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE». Acta Biológica Colombiana 29 (2):5-11. https://doi.org/10.15446/abc.v29n2.99155.

Harvard

Tikirdik, M., Mutlu, A. G., Alkan, I., Yildiz, H., Korkmaz, D. y Topal, A. (2023) «THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE», Acta Biológica Colombiana, 29(2), pp. 5–11. doi: 10.15446/abc.v29n2.99155.

IEEE

[1]
M. Tikirdik, A. G. Mutlu, I. Alkan, H. Yildiz, D. Korkmaz, y A. Topal, «THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE», Acta biol. Colomb., vol. 29, n.º 2, pp. 5–11, dic. 2023.

MLA

Tikirdik, M., A. G. Mutlu, I. Alkan, H. Yildiz, D. Korkmaz, y A. Topal. «THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE». Acta Biológica Colombiana, vol. 29, n.º 2, diciembre de 2023, pp. 5-11, doi:10.15446/abc.v29n2.99155.

Turabian

Tikirdik, Muazzez, Ayse Gul Mutlu, Irem Alkan, Hulya Yildiz, Didem Korkmaz, y Aykut Topal. «THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE». Acta Biológica Colombiana 29, no. 2 (diciembre 27, 2023): 5–11. Accedido marzo 6, 2025. https://revistas.unal.edu.co/index.php/actabiol/article/view/99155.

Vancouver

1.
Tikirdik M, Mutlu AG, Alkan I, Yildiz H, Korkmaz D, Topal A. THE DOSE-DEPENDENT EFFECTS OF CAFFEINE AND BETAINE ON TELOMERASE ENZYME ACTIVITY IN MICE. Acta biol. Colomb. [Internet]. 27 de diciembre de 2023 [citado 6 de marzo de 2025];29(2):5-11. Disponible en: https://revistas.unal.edu.co/index.php/actabiol/article/view/99155

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