Ojo blanco (obl): un gene marcador del grupo de ligamiento IV en Tribolium castaneum Herbst
Keywords:
Genetica Insectos, Genetica Tribolium, Ligamiento Genetico, Mapeo Cromosómico, Coleóptera, Tenebrionidae, Tribolium (es)Downloads
Se estudiaron algunos aspectos genéticos de una mutación espontanea en T. casteneum, descubierta en el Departamento de Biología de la Universidad Nacional de Colombia (Bogotá). EI gene mutante llamado "ojo blanco" (obl) es autosómico recesivo, de excelente viabilidad, penetrancia completa y expresividad variable. Fenotípicamente se caracteriza por una despigmentación de los ojos compuestos; puede observarse desde el estado de larva. Se le hicieron pruebas de ligamiento con marcadores para diferentes grupos. Se obtuvieron resultados positivos con el marcador "sooty" del grupo de ligamiento IV. La distancia se calcu1ó a partir de 2.843 descendientes de retrocruzas en posición de repulsión y 1.630 descendientes de retrocruzas en posición de acoplamiento. Esta distancia fue de 15.ó4 ± 0.92 unidades entre obl ys. No se pudo determinar si obl esta a la izquierda 0 a la derecha de s. No se hallaron discrepancias entre los sexos ni entre las fases en la frecuencia de recombinación para la región del cromosoma IV comprendida entre los loci. obl ys.
Some genetic aspects of a spontaneous mutation in T. castaneum, discovered in the Department of Biology of National University of Colombia (Bogota), were studied. The mutant gene christened "ojo blanco" (obl), is recessive autosomic, of excellent viability, complete penetrance and variable expression. The gene is phenotipically characterized by a depigmentation of the compound eyes and can be observed from the larval stage. Linkage tests with marker mutants for different groups were carried out. The marker "sooty", of the linkage group IV, gave positive results. The distance was estimated from 2843 descendants obtained from backcrosses in repulsion plase and 1630 in coupling phase. This distance was worked out as 15.64 ± 0.92 units between obl ands. It was not possible to determine if obl is on the left or the right hand side of s. Nor discrepancies between sexes, neither between phases were found in the recombination frequencies for the region between the two loci of chromosome IV.
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