Published

2006-07-01

Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus

Keywords:

anti-freezing proteins, Senecio niveoaureus, high tropical Andes (páramo). (en)

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Authors

  • Fagua Virginia Álvarez Flórez Universidad Nacional de Colombia - Sede Bogotá.
Tropical high mountain plants have different adaptations to survive extreme daily temperature fluctuations and specially freezing night conditions. In winter plant species, survival to low temperatures is related to the ability of the cell to produce specific low molecular weight proteins (anti-freezing proteins) and to export them to the apoplast. In order to see if high mountain tropical plants survive to low temperatures through the same mechanism we collected, during a 24 hour-period, leaves from Senecio niveoaureus growing at 3,300 and 3,600 m.o.s.l, in the Páramo de Palacio, Chingaza, Colombia. Leaf apoplast proteins had MW between 35-12 kDa. Electrophoretic patterns were different depending on the altitude and the time of sampling. However the observed variations could not be linked to changes in temperature or to the altitudinal gradient. Antifreeze activity was detected in leaf apoplast of plants at different altitudes. This is the first report of anti-freeze activity in a high mountain tropical species.

References

ANTIKAINEN M, GRIFFITH M, ZHANG J, HON W, YANG D, PIHAKASKI-MAUNSBACH

K. Immunolocalisation Of Antifreeze Proteins In Winter Rye Leaves, Crowns, And Roots ByTissue Printing. Plant Physiol. 1996;110:845-857.

ANTIKAINEN M, GRIFFITH M. Antifreeze Protein Accumulation in Freezing- Tolerant Cereals. Physiol Plant. 1997;99:423-432.

BECK E, SENSER M, SCHEIBE R, STEIGER H, PONGRATZ P. Frost Avoidance And Freezing Tolerance In Afroalpine "Giant Rosette" Plants. Plant Cell Environ. 1982;5:215-222.

BLUM H, BEIER H, GROSS HJ. Improved Silver Staining of Plant Proteins Rna and Dna in Polyacrylamide Gel Electrophoresis. Electrophoresis. 1987;8:93-99.

BRADFORD M. A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principie of Protein-Dye Binding. Anal Biochem. 1976;72:248-254.

DE VRIES AL. Antifreeze Glycopeptides And Peptides: Interactions With Ice And Water. Meth Enzymol. 1986;127:293-303.

GOLDSTEIN G, RADA F, CANALES M, ZABALA O. LeafGas Exchange of Two Giant Caulescent Rosette Species. Acta Oecologica. 1989;10:359-370.

GRIFFITH M, EWART V. Antifreeze Proteins and their Potential Use in Frozen Foods. Biotechnol Adv. 1995;13:375-402.

GRIFFITH M, ANTIKAINEN M, HON W, PIHAKASKI-MAUNSBACH K, YU X, CHUN J, et al. Antifreeze Proteins in Winter Rye. Physiol Plant. 1997;100: 327-332.

GRIFFITH M, LUMB C, WISEMAN SB, WISNIEWSKY M, JOHNSON R, MARANGONI A. Antifreeze Proteins Modify The Freezing Process in Planta. Plant Physiol. 2005;138:330-340.

HON W, GRIFFITH M, CHONG P, YANG D. Extraction and Isolation of Antifreeze Proteins from Winter Rye (secale cereale l.) Leaves. Plant Physiol. 1994; 104:971-980.

HON W, GRIFFITH M, MLYNARZ A, KWOK Y, YANG D. Antifreeze Proteins in Winter Rye are Similar to Pathogenesis-Related Proteins. Plant Physiol. 1995;109:879-889.

KORNER C. Alpine Plant Life. Germany: Springer; 1999.

LAEMMLI U K. Cleavage of Structural Proteins During the Assembly of the Head of Bacteriophage T4. Nature. 1970;227:680-685.

LUTEYN J. Paramos A Checklist Of Plant Diversity, Geographical Distribution, and Botanical Literature. The New York Botanical Garden, USA; 1999.

LÜTTGE U. Physiological Ecology of Tropical Plants. Germany: Springer; 1997. p. 349-371.

MORA-OSEJO L, H. STURM. Estudios ecológicos del páramo y del bosque altoandino cordillera Oriental Colombiana. Academia Colombiana de Ciencias Exactas, Físicas y Naturales. Bogotá, Colombia. 1994. p. 5-25.

PIHAKASKI-MAUNSBACH K, GRIFFITH M, ANTIKAINEN M, MAUNSBACK AB. Immunogold Localization of Glucanase-Like Antifreeze Protein in Cold Acclimated Winter Rye. Protoplasma. 1996; 191: 115-125.

RADA F, GOLDSTEIN G, AZOCAR A, MEINZER F. Freezing Avoidance in Andean Giant Rosette Plants. Plant Cell Environ. 1985;8:501-507.

RADA F, GOLDSTEIN G, AZOCAR A, TORRES F. Supercooling Along an Altitudinal Gradient In Espeletia schultzii, A Caulescent Giant Rosette Species. J Exp Bot. 1987;38:491-497.

RADA F, GARCíA NC, BOERO C, GALLARDO M, HILAL M, GONZÁLEZ J, et at. Low Temperature Resistance In Polylepis tarapacana, A Tree Growing at the Highes Altitudes in the World. Plant Cell Environ. 2001;24:377-381.

SMALLWOOD M, WORRALL D, BYASSL, ELIAS L, ASHFORD D, DOUCET J, et al. Isolation and Characterization of a Novel Antifreeze Protein from Carrot (Daucus carota). Biochem j. 1999;340:385-391.

STOSCHECK CM. Increased Uniformity in the Response of the Coomasie Blue G Protein Assay to Different Proteins, Anal Biochem. 1990; 184:111-116.

WORRALL D, EllAS L, ASHFORD D, SMALLWOOD M, SIDEBOTTOM C, L1LLFORD P, et al. A Carrot Leucine-Rich-Repeat Protein that Inhibits Ice Re-Crystallization. Science. 1998;282: 115-117.

How to Cite

APA

Álvarez Flórez, F. V. (2006). Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus. Acta Biológica Colombiana, 11(2), 103–111. https://revistas.unal.edu.co/index.php/actabiol/article/view/63333

ACM

[1]
Álvarez Flórez, F.V. 2006. Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus. Acta Biológica Colombiana. 11, 2 (Jul. 2006), 103–111.

ACS

(1)
Álvarez Flórez, F. V. Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus. Acta biol. Colomb. 2006, 11, 103-111.

ABNT

ÁLVAREZ FLÓREZ, F. V. Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus. Acta Biológica Colombiana, [S. l.], v. 11, n. 2, p. 103–111, 2006. Disponível em: https://revistas.unal.edu.co/index.php/actabiol/article/view/63333. Acesso em: 17 jan. 2025.

Chicago

Álvarez Flórez, Fagua Virginia. 2006. “Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus”. Acta Biológica Colombiana 11 (2):103-11. https://revistas.unal.edu.co/index.php/actabiol/article/view/63333.

Harvard

Álvarez Flórez, F. V. (2006) “Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus”, Acta Biológica Colombiana, 11(2), pp. 103–111. Available at: https://revistas.unal.edu.co/index.php/actabiol/article/view/63333 (Accessed: 17 January 2025).

IEEE

[1]
F. V. Álvarez Flórez, “Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus”, Acta biol. Colomb., vol. 11, no. 2, pp. 103–111, Jul. 2006.

MLA

Álvarez Flórez, F. V. “Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus”. Acta Biológica Colombiana, vol. 11, no. 2, July 2006, pp. 103-11, https://revistas.unal.edu.co/index.php/actabiol/article/view/63333.

Turabian

Álvarez Flórez, Fagua Virginia. “Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus”. Acta Biológica Colombiana 11, no. 2 (July 1, 2006): 103–111. Accessed January 17, 2025. https://revistas.unal.edu.co/index.php/actabiol/article/view/63333.

Vancouver

1.
Álvarez Flórez FV. Protein Electrophoretic Patterns and Anti-Freezing Activity in the Leaf Apoplast of The Tropical Andean Species Senecio Niveoaureus. Acta biol. Colomb. [Internet]. 2006 Jul. 1 [cited 2025 Jan. 17];11(2):103-11. Available from: https://revistas.unal.edu.co/index.php/actabiol/article/view/63333

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