Correo Electrónico
DNINFOA - SIA
Bibliotecas
Convocatorias
Identidad U.N.
Published
Detección por PCR de Haemoproteus archilochus en Amazilia tzacatl (Trochilidae) en Colombia
PCR detection of Haemoproteus archilochus in Rufous-tailed Hummingbird Amazilia tzacatl (Trochilidae) in Colombia.
DOI:
https://doi.org/10.15446/abc.v27n1.89162Keywords:
sangre, parásitos, infecciones, biología molecular, Aves (es)Birds, blood, infections, parasites, molecular biology (en)
Downloads
La infección causada por haemosporidios en colibríes no ha sido estudiada en zonas agroforestales o urbanas de la vertiente occidental de la Cordillera Oriental de los Andes en el departamento de Santander, pese a existir evidencia de esta en otros grupos de aves. Con el fin de detectar e identificar los parásitos causales de infecciones por haemosporidios, se tomaron muestras de sangre de la vena yugular de colibríes en seis localidades. La presencia de infección se llevó a cabo por PCR y la identificación de los parásitos se hizo a partir de secuencias del gen mitocondrial Citocromo b (Cyt b). Se obtuvieron 86 muestras de sangre de 20 especies de colibríes. La prevalencia de infección en general fue del 43 % y en el 18 % de las muestras infectadas del colibrí Amazilia colirufa (Amazilia tzacatl) se identificaron secuencias de Haemoproteus archilochus correspondientes al linaje HUMHA4. Se reporta por primera vez para Colombia la presencia de H. archilochus en A. tzacatl, por medio de técnicas de biología molecular. Este parásito podría estar implicado en la haemoproteosis de colibríes en el país.
Haemosporidian infection in hummingbirds has not been studied in agroforestry or urban areas of the Eastern Andes' western slope, in the department of Santander, despite the existence of evidence of this in other groups of birds. To detect and identify the causative parasites of haemosporidium infections, we took blood samples from the jugular vein of hummingbirds in six locations. PCR carried out the diagnosis of the infection, and the identification of the parasites was made from sequences of the mitochondrial gene Cytochrome b (Cyt b). 86 blood samples were obtained from 20 species of hummingbirds. The prevalence of infection, in general, was 43 %, and in 18 % of the infected samples of Rufous-tailed Hummingbird (Amazilia tzacatl), sequences of HUMHA4 lineage from Haemoproteus archilochus were identified. The presence of H. archilochus in A. tzacatl is reported for the first time in Colombia using molecular biology techniques. This parasite could be implicated in the haemoproteosis of hummingbirds in the country.
References
Altschul, S. F., Gish, W., Mille, W., Myers, E. W., y Lipman, D. J. (1990). Basic local alignment search tool. Journal Molecular Biology, 215(3), 403-410. https://doi.org/10.1016/S0022-2836(05)80360-2 DOI: https://doi.org/10.1016/S0022-2836(05)80360-2
Bennett, G. F., y Borrero, H. J. I. (1976). Blood Parasites of Some Birds From Colombia. Journal Wildl Diseases, 12(3), 454-458. https://doi.org/10.7589/0090-3558-12.3.454 DOI: https://doi.org/10.7589/0090-3558-12.3.454
Bradshaw, A. C, Tell, L, A., Ernest, H. B., Bahan, S., Carlson, J., y Sehgal, R. N. M. (2017). Detection and prevalence of Haemoproteus archilochus (Haemosporida, Haemoproteidae) in two species of California hummingbirds. Parasitology Research, 116(7), 1879-1885. https://doi.org/10.1007/s00436-017-5463-5 DOI: https://doi.org/10.1007/s00436-017-5463-5
Coatney, G. R., y West, E. (1938). Some Blood Parasites from Nebraska Birds. II. The American Midland Naturalist, 19(3), 601-612. https://doi.org/10.2307/2420473 DOI: https://doi.org/10.2307/2420473
Fallon, S. M., y Rickfls, R. E. (2008). Parasitemia in PCR-detected Plasmodium and Haemoproteus infections in birds. Journal of Avian Biology, 39(5), 514-522. https://doi.org/10.1111/j.0908-8857.2008.04308.x DOI: https://doi.org/10.1111/j.0908-8857.2008.04308.x
Fallon, S. M., Ricklefs, R. E., Swanson, B. L., y Bermingham, E. (2003). Detecting Avian Malaria: An Improved Polymerase Chain Reaction Diagnostic. Journal of Parasitololy, 89(5), 1044-1047. https://doi.org/10.1645/GE-3157 DOI: https://doi.org/10.1645/GE-3157
Godoy, L. A., Tell, L. A., y Ernes, H. B. (2014). Hummingbird health: pathogens and disease conditions in the family Trochilidae. Journal of Ornithology, 155(1), 1-12. https://doi.org/10.1007/s10336-013-0990-z DOI: https://doi.org/10.1007/s10336-013-0990-z
González, A. D., Lotta, I. A., García, L. F., Moncada, L. I., y Matta, N. E. (2015). Avian haemosporidians from Neotropical highlands: Evidence from morphological and molecular data. Parasitology International, 64(4), 48-59. https://doi.org/10.1016/j.parint.2015.01.007 DOI: https://doi.org/10.1016/j.parint.2015.01.007
González, A. D, Matta, N. E., Ellis, V. A., Miller, E. T, Ricklefs, R. E, y Gutiérrez, H. R. (2014). Mixed Species Flock, Nest Height, and Elevation Partially Explain Avian Haemoparasite Prevalence in Colombia. PLOS ONE, 9(6), e100695. https://doi.org/10.1371/journal.pone.0100695 DOI: https://doi.org/10.1371/journal.pone.0100695
Gonzalez-Quevedo, C., Pabón, A., y Rivera-Gutierrez, H. F. Prevalence of haemosporidians in a Neotropical endemic bird area. Avian Conservation and Ecology, 11(1), 7. https://doi.org/10.5751/ACE-00834-110107 DOI: https://doi.org/10.5751/ACE-00834-110107
Green, M. R., y Sambrook, J. (2012). Molecular Cloning: A Laboratory Manual (4 ed.). Spring Harbor Laboratory Press. Cold Spring Harbor.
Harrigan, R. J., Sedano, R., Chasar, A. C., Chaves, J. A., Nguyen, J. T., Whitaker, A., y Smith, T. B. (2014). New host and lineage diversity of avian haemosporidia in the northern Andes. Evolutionary Applications, 7(7), 799-811. https://doi.org/10.1111/eva.12176 DOI: https://doi.org/10.1111/eva.12176
Hellgren, O., Waldenström, J., y Bensch, S. (2004). A New PCR Assay for Simultaneous Studies of Leucocytozoon, Plasmodium, and Haemoproteus from Avian Blood. Journal of Parasitology, 90(4), 797-802. https://doi.org/10.1645/GE-184R1 DOI: https://doi.org/10.1645/GE-184R1
Lotta, I. A., Gonzalez, A. D., Pacheco, M. A., Escalante, A. A., Valkiūnas, G., Moncada, I. M, y Matta, N. E. (2015). Leucocytozoon pterotenuis sp. nov. (Haemosporida, Leucocytozoidae): description of the morphologically unique species from the Grallariidae birds, with remarks on the distribution of Leucocytozoon parasites in the Neotropics. Parasitology Research, 114(3), 1031-1044. https://doi.org/10.1007/s00436-014-4269-y DOI: https://doi.org/10.1007/s00436-014-4269-y
Lotta, I. A., Pacheco, M. A., Escalante, A. A., González, A. D., Mantilla, J. S., Moncada, L. I., y Matta, N. E. (2016). Leucocytozoon Diversity and Possible Vectors in the Neotropical highlands of Colombia. Protist, 167(2), 185-204. https://doi.org/10.1016/j.protis.2016.02.002 DOI: https://doi.org/10.1016/j.protis.2016.02.002
Mantilla, J. S., Matta, N. E., Pacheco, M. A., Escalante, A. A., González, A. D., y Moncada, L. I. (2013). Identification of Plasmodium (Haemamoeba) lutzi (Lucena, 1939) from Turdus fuscater (Great Thrush) in Colombia. Journal of Parasitology, 99(4), 662-668. https://doi.org/10.1645/12-138.1 DOI: https://doi.org/10.1645/12-138.1
Marzal, A., García-Longoria, L., Cárdenas Callirgos, J. M., y Sehgal, R, N. (2014). Invasive avian malaria as an emerging parasitic disease in native birds of Peru. Biological Invasions, 17(1), 39-45. https://doi.org/10.1007/s10530-014-0718-x DOI: https://doi.org/10.1007/s10530-014-0718-x
Matta, N. E., Lotta, I. A., Valkiūnas, G., González, A. D., Pacheco, M. A., Escalante, A. A., Moncada, L. I, y Rodríguez-Fandiño, O. A. (2014). Description of Leucocytozoon quynzae sp. nov. (Haemosporida, Leucocytozoidae) from hummingbirds, with remarks on distribution and possible vectors of leucocytozoids in South America. Parasitology Research, 113(2), 457-468. https://doi.org/10.1007/s00436-013-3675-x DOI: https://doi.org/10.1007/s00436-013-3675-x
McMullan, M. (2018). The Field Guide to the Birds of Colombia. Rey Naranjo Editores S.A.S.
Mijares, A., Rosales, R., y Silva-Iturriza, A. Hemosporidian Parasites in Forest Birds from Venezuela: Genetic Lineage Analyses. Avian Diseases, 56(3), 583-588. https://doi.org/10.1637/10058-011312-ResNote.1 DOI: https://doi.org/10.1637/10058-011312-ResNote.1
Moens, M. A. J., Valkiūnas, G., Paca, A., Bonaccorso, E., Aguirre, N., y Pérez‐Tris, J. (2016). Parasite specialization in a unique habitat: hummingbirds as reservoirs of generalist blood parasites of Andean birds. Journal of Animal Ecology, 85(5), 1234-1245. https://doi.org/10.1111/1365-2656.12550 DOI: https://doi.org/10.1111/1365-2656.12550
Owen, J. C. (2015). Collecting, processing, and storing avian blood: a review. Journal of Field Ornithology, 82(4), 339-354. https://doi.org/10.1111/j.1557-9263.2011.00338.x DOI: https://doi.org/10.1111/j.1557-9263.2011.00338.x
Pérez-Tris, J., Hasselquist, D., Hellgren, O., Križanauskienė, A., Waldenström, J., y Bensch, S. (2005). What are malaria parasites? Trends Parasitol, 21(5), 209-211. https://doi.org/10.1016/j.pt.2005.03.001 DOI: https://doi.org/10.1016/j.pt.2005.03.001
Rodríguez, O. A., Moya, H., y Matta, N. E. (2009). Avian blood parasites in the National Natural Park Chingaza: high Andes of Colombia. Hornero, 24(1):1-6.
Santos, F. R., Pena, S. D., y Epplen, J. T. (1993). Genetic and population study of a Y-linked tetranucleotide repeat DNA polymorphism with a simple non-isotopic technique. Human Genetics, 90(6), 655-656. https://doi.org/10.1007/BF00202486 DOI: https://doi.org/10.1007/BF00202486
Valkiūnas, G., Iezhova, T. A., Brooks, D. R., Hanelt, B., Brant, S. V., Sutherlin, M. E-, y Causey, D. (2004). Additional Observations on Blood Parasites of Birds in Costa Rica. Journal Wildlife Diseases, 40(3), 555-561. https://doi.org/10.7589/0090-3558-40.3.555 DOI: https://doi.org/10.7589/0090-3558-40.3.555
Valkiūnas G. (2005). Avian Malaria Parasites and other Haemosporidia (pp. 1-5). CRC Press. https://doi.org/10.1201/9780203643792.ch0 DOI: https://doi.org/10.1201/9780203643792.ch0
Villamizar-Escalante, D. (2017). Prevalencia y diversidad de hemoparásitos causantes de malaria en comunidades de aves del departamento de Santander, Colombia [Tesis de pregrado]. Escuela de Biología, Facultad de Ciencias, Universidad Industrial de Santander.
Villareal, H., Álvarez, M., Córdoba, S., Escobar, F., Fagua, G., Gast, H., Mendoza, M. Ospina, M., y Umaña, A. M. (2004). Manual de métodos para el desarrollo de inventarios de biodiversidad. Programa de inventarios de Biodiversidad. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt.
How to Cite
APA
ACM
ACS
ABNT
Chicago
Harvard
IEEE
MLA
Turabian
Vancouver
Download Citation
CrossRef Cited-by
1. Harold F. Greeney, Brooke K. Keeney. (2023). Birds of the World. https://doi.org/10.2173/bow.bltman1.02.
2. Valeria Landinez-Álvarez, Esteban Álvarez-Betancur, Gloria Yaneth Sánchez-Zapata, Cristina Úsuga-Monroy, Daisy A. Gómez-Ruiz. (2024). Descripción de hemoparásitos en aves silvestres de áreas rurales del Valle de Aburrá y zonas cercanas. Revista de Medicina Veterinaria, https://doi.org/10.19052/mv.vol1.iss49.7.
Dimensions
PlumX
Article abstract page views
Downloads
License
Copyright (c) 2021 Acta Biológica Colombiana
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
The acceptance of manuscripts by the Journal implies its electronic edition of open Access under Creative Commons Attribution License 4.0, and the inclusion and diffusion of the complete text through the institutional repository of the Universidad Nacional de Colombia and in all the specialized data bases that the editor considers adequate for its indexation to increase Journal visibility.
Acta Biológica Colombiana allows authors to archive, download and distribute the final published version, as well as pre-print and post-print versions including a header with the bibliographic reference of published article. The journal encourages the authors to distribute the final versión through Internet, for example in their personal or institutional web pages, and scientific social networks.
