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Biofertilizer enhances the expression of the CaWRKY6 gene in water-stressed plants of Capsicum anuum L.
Biofertilizante mejora la expresión del gen CaWRKY6 en plantas de Capsicum anuum L. sometidas a estrés hídrico
DOI:
https://doi.org/10.15446/agron.colomb.v43n2.118856Keywords:
organic agriculture, organic fertilizers, abiotic stress resistance, drought protection (en)agricultura orgánica, fertilizantes orgánicos, resistencia a estrés abiótico, protección contra sequía (es)
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Colombia has agroecological conditions for bell pepper (Capsicum annuum L.) cultivation. However, traditional management practices have depended mainly on agrochemicals. Organic production could enhance the value of products and facilitate their entry into international markets. Research indicates that biofertilizers and biostimulants can activate the gene expression that helps plants tolerate both biotic and abiotic stress. This study assessed the activation of the CaWRKY6 gene in response to water stress tolerance in C. annuum, after applying a biofertilizer to greenhouse-grown young plants to mitigate the impacts of drought. Foliar samples were taken 48 h after the application of five treatments: biofertilizer at two concentrations (5 ml L-1 and 10 ml L-1), salicylic acid (5 mg L-1), and two controls (water), followed by RNA extraction and an RT-qPCR test to determine the relative expression of the gene CaWRKY6. To compare the adjuvant effect of biofertilizer and salicylic acid, treated young plants were exposed to water depletion for 21 d. The ANOVA indicated differences between treatments with 5 ml L-1 and 10 ml L-1 of biofertilizer, and they had the highest CaWRKY6 gene expression, as well as higher growth and less wilting against water stress.
Colombia presenta condiciones agroecológicas para la producción de pimentón (Capsicum annuum L.). Sin embargo, su manejo tradicional ha sido principalmente por medio del uso de agroquímicos. La producción orgánica podría dar valor agregado a los productos, y facilitar su entrada a los mercados internacionales. Se ha evidenciado que los biofertilizantes y bioestimulantes pueden activar la expresión de genes que ayudan a las plantas a tolerar estrés biótico y abiótico. En este estudio se evaluó la activación del gen CaWRKY6, uno de los más destacados para C. annuum, tras la aplicación de un biofertilizante sobre plántulas de pimentón cultivadas bajo invernadero. Se analizaron muestras foliares 48 h después de aplicar cinco tratamientos: biofertilizante a dos concentraciones (5 ml L-1 y 10 ml L-1), ácido salicílico (5 mg L-1) y dos controles (agua). Después se extrajo ARN y mediante una prueba RT-qPCR se determinó la expresión relativa del gen CaWRKY6. Para comparar el efecto adyuvante del biofertilizante y el ácido salicílico, las plantas jóvenes tratadas se expusieron a la falta de agua durante 21 d. El ANOVA indicó diferencias entre los tratamientos con 5 ml L-1 y 10 ml L-1 de biofertilizante; además, estos tuvieron la mayor expresión del gen CaWRKY6, mayor crecimiento y menor marchitez por el estrés hídrico.
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