Published

2014-05-01

Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia

Co-infección natural de Potato yellow vein virus y potivirus en cultivos de Solanum tuberosum en Colombia

DOI:

https://doi.org/10.15446/agron.colomb.v32n2.43968

Keywords:

viral diseases, Potato virus V, prevalence, NGS, potato, root vegetables. (en)
enfermedades virales, Potato virus V, prevalencia, NGS, papa, hortalizas de raíz (es)

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Authors

  • Angela Villamil-Garzón Universidad Nacional de Colombia - Sede Bogotá - Plant Virus Laboratory - Biotechnology Institute
  • Wilmer J. Cuellar International Center for Tropical Agriculture (CIAT)
  • Mónica Guzmán-Barney Universidad Nacional de Colombia - Sede Bogotá - Plant Virus Laboratory - Biotechnology Institute
The Potato yellow vein virus (PYVV), a Crinivirus with an RNA tripartite genome, is the causal agent of the potato yellow vein disease, reported in Colombian since 1950, with yield reductions of up to 50%. Co-infection of two or more viruses is common in nature and can be associated with differences in virus accumulation and symptom expression. No evidence of mixed infection between PYVV and other viruses has been reported. In this study, eight plants showing yellowing PYVV symptoms: four Solanum tuberosum Group Phureja (P) and four Group Andigena (A), were collected in Cundinamarca, Colombia to detect mixed infection in the isolates using next generation sequencing (NGS). The Potato virus Y (PVY) complete genome (similar to N strain) and the Potato virus V (PVV) partial genomes were detected using NGS and re-confirmed by RT-PCR. Preliminary field screening in a large sample showed that PYVV and PVY co-infect potato plants with a prevalence of 21% within the P group and 23% within the A group. This is the first report of co-infection of PYVV and potyvirus in Colombia and with the use of NGS. Considering that potyviruses enhance symptom severity and/or yield reductions in mixed infections, our results may be relevant for disease diagnosis, breeding programs and tuber certification.
El Potato yellow vein virus (PYVV) es un Crinivirus de genoma RNA tripartita, agente causal de la enfermedad del amarillamiento de las nervaduras de la papa reportada desde 1950 en Colombia con reducción de producción hasta 50%. La coinfección entre virus en un mismo hospedero es común en la naturaleza y se asocia con cambios en acumulación viral y en la expresión de síntomas. Hasta el momento no se ha reportado coinfección entre PYVV y otros virus en papa. En este estudio se colectaron en Cundinamarca-Colombia ocho plantas con síntomas de PYVV: cuatro Solanum tuberosum Grupo Phureja (P) y cuatro del Grupo Andigena (A). Utilizando secuenciamiento de alto rendimiento (NGS) en los aislamientos se detectó infección mixta de PYVV con potyvirus que se reconfirmó con RT-PCR. Estudios preliminares en una muestra mayor de campo confirmaron que PYVV y PVY coinfectan papa con 21% de prevalencia para el grupo P y 23% para el grupo A. Este es el primer reporte de coinfección de PYVV y potyvirus en Colombia. Teniendo en cuenta que los potyvirus pueden incrementar la severidad de síntomas y/o reducir el rendimiento en infecciones mixtas, los resultados presentados pueden ser relevantes para el diagnóstico de enfermedades virales, programas de propagación y de certificación de semillas.

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Villamil-Garzón, A., Cuellar, W. J., & Guzmán-Barney, M. (2014). Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia. Agronomía Colombiana, 32(2), 213-223. https://doi.org/10.15446/agron.colomb.v32n2.43968

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