Identification of the variability of a common bean collection through morphological, physiological, biochemical, and molecular relationships

Published

2014-05-01

Identification of the variability of a common bean collection through morphological, physiological, biochemical, and molecular relationships

Identificación de la variabilidad de una colección de fríjol común mediante la relación de parametros morfológicos, fisiológicos, bioquímicos y moleculares

Keywords:

Grain legumes, germplasm, genetic variation, isoenzymatic, molecular markers. (en)
leguminosas de grano, germoplasma, variación genética, isoenzimas, marcadores moleculares. (es)

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Authors

Gustavo A. Ligarreto M. Universidad Nacional de Colombia - Sede Bogotá - Faculty of Agricultural Sciences - Departament of Agronomy
Orlando Martínez W. Private consultant in Biostatistical Analysis

Abstract (en)
Abstract (es)

The present study was carried out in Corpoica, C.I. Tibaitata (Mosquera, Colombia) at an altitude of 2,540 m a.s.l. in six environments composed of different seasons and at an altitude of 1,485 m a.s.l. in the International Center for Tropical Agriculture (CIAT ), Calima, Colombia. Morphological, physiological, biochemical, and molecular descriptors were used to estimate the genetic variability between 36 Colombian bean accessions, of which four were wild and the others cultivated. Diacol Calima (Nueva Granada from the Andean gene pool) and ICA Pijao (Mesoamerican from the mesoamerican gene pool) were used as controls. The combined analysis of the qualitative and quantitative variables was carried out with Gower distance and an unified data matrix with 315 descriptors. The relationships between the genetic distances differentiated the bean collection into two genetic groups: Andean and Mesoamerican. The following groups of characteristics presented high association: total morphology with qualitative morphology (P=0.91), physiological evaluation with grouped evaluation of morphological, quantitative, and physiological characteristics (P=0.91), characterization of isoenzymatic and molecular markers with respect to just molecular markers (P=0.99) and the characterization of all the studied markers in relation to the molecular and isoenzymatic markers (P=0.88).

El trabajo se realizó en Corpoica, C.I. Tibaitatá (Mosquera, Colombia), a 2.540 msnm, de altitud, en seis ambientes constituidos por diferentes localidades y semestres y a una altidud de 1.485 msnm en el Centro Internacional de Agricultura Tropical (CIAT ) en Calima, Colombia. Se usaron descriptores morfológicos, fisiológicos, bioquímicos y moleculares para estimar la variabilidad genética entre 36 accesiones colombianas de fríjol, de las cuales cuatro son silvestres y las otras cultivadas. Se incluyen los testigos Diacol Calima (raza Nueva Granada) e ICA Pijao (raza Mesoamerica), uno de cada acervo genético. El análisis mixto de variables cualitativas y cuantitativas se efectuó con la distancia Gower, para el efecto, en una misma matriz de datos se unieron 315 descriptores. Las relaciones entre las distancias genéticas discriminaron la colección de fríjol en dos grupos genéticos andinos y mesoamericanos. Se presentó alta asociación entre las caracterizaciones siguientes: morfológica total con morfológica cualitativa (P=0,91), evaluación fisiológica con la evaluación conjunta de caracteres morfológicos cuantitativos y fisiológicos (P=0,91), caracterización de marcadores isoenzimáticos más moleculares con respecto a sólo marcadores moleculares (P=0,99) y en la caracterización de todos los marcadores estudiados en relación con los moleculares y moleculares más isoenzimas (P=0,88).

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