Published

2014-09-01

Micropropagation of the native species Anthurium antioquiense Engl. for conservation purposes

Micropropagación de la especie nativa Anthurium antioquiense Engl. para su conservación

DOI:

https://doi.org/10.15446/agron.colomb.v32n3.46809

Keywords:

Anthurium, growth regulators, in vitro culture, preservation, propagation, shoots (en)
Anthurium, reguladores de crecimiento, cultivo in vitro, preservacion, propagacion, brotes (es)

Authors

  • Paola Andrea Murillo-Gómez Universidad de Antioquia - Biology Institute - Biotechnology Group
  • Esther Naranjo Universidad de Antioquia - Biology Institute - Biotechnology Group
  • Ricardo Callejas Universidad de Antioquia - Biology Institute - Biotechnology Group
  • Lucia Atehortúa Universidad de Antioquia - Biology Institute - Biotechnology Group
  • Aura Urrea Universidad de Antioquia - Biology Institute - Biotechnology Group
Anthurium antioquiense Engl. is a native plant belonging to the Araceae family. It grows on rocks in clear-water rivers and well-protected zones, similar to the waters in certain watersheds of the Antioquia Department, Colombia. Loss of habitat has threatened this promising ornamental plant species, which is also important because of its role in the ecosystem. In vitro tissue culture is considered an efficient alternative for the propagation of endangered species with the aim of establishing short-, medium- and long-term conservation programs. In the present research, in vitro introduction and shoot induction from A. antioquiense seedlings were performed. The highest production of shoots was obtained in a ½ MS (half-salt content) medium with 1 mg L-1 of BAP, which attained a 23.7 shoots/explant per month multiplication rate. The in vitro plants generated from shoots were individualized and transferred to a growth regulator-free medium. Rooting did not require the presence of growth regulators, and the adaptation of the in vitro plants to ex vitro conditions achieved a 98% survival rate.
Anthurium antioquiense Engl. es una planta nativa perteneciente a la familia Araceae. Crece sobre rocas en ríos de aguas claras y en zonas protegidas, características de algunas cuencas hidrográficas del departamento de Antioquia-Colombia. La pérdida de hábitat amenaza esta promisoria especie ornamental, la cual también es importante debido a su incidencia en el ecosistema. El cultivo de tejidos in vitro se considera una alternativa eficiente para la propagación de las especies en peligro de extinción, con el fin de establecer programas de conservación a corto, mediano o largo plazo. En el presente trabajo de investigación, se llevó a cabo la introducción e inducción de brotes in vitro de A. antioquiense. La mayor producción de brotes se obtuvo en medio ½ MS (contenido de sales a la mitad) con 1 mg L-1 de BAP (23,7 brotes/explante por mes). Las plantas in vitro obtenidas a partir de los brotes fueron individualizadas y transferidas a medio libre de reguladores de crecimiento. El enraizamiento se realizó sin presencia de reguladores de crecimiento, y la adaptación de las plantas in vitro a condiciones ex vitro alcanzó una tasa de supervivencia del 98%.

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How to Cite

Micropropagation of the native species Anthurium antioquiense Engl. for conservation purposes. (2014). Agronomía Colombiana, 32(3), 334-340. https://doi.org/10.15446/agron.colomb.v32n3.46809