Published

2014-09-01

Molecular characterization of 93 genotypes of cocoa (Theobroma cacao L.) with random amplified microsatellites RAMs

Caracterización molecular con microsatélites amplificados al azar (RAMs) de 93 genotipos de cacao (Theobroma cacao L.)

DOI:

https://doi.org/10.15446/agron.colomb.v32n3.46879

Keywords:

Malvaceae, molecular markers, heterozygosity, clonal multiplication, genetic diversity (en)
Malvaceae, marcadores moleculares, heterocigosidad, multiplicación clonal, diversidad genética (es)

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Authors

  • Yacenia Morillo C. Universidad de los Llanos - Faculty of Agricultural Sciences and Natural Resources
  • Ana Cruz Morillo C. Universidad Pedagogica y Tecnologica de Colombia (UPTC) - Faculty of Agricultural Sciences
  • Jaime Eduardo Muñoz F. Universidad Nacional de Colombia - Sede Palmira - Faculty of Agricultural Sciences
  • William Ballesteros P. Universidad de Nariño - Pasto - Faculty of Agricultural Sciences
  • Alonso González Corporación Colombiana de Investigación Agropecuaria (Corpoica) - Tibaitata Research Center
Random amplified microsatellite (RAMs) markers six were used to characterize 93 genotypes of cocoa in Tumaco (Colombia). Hundred twenty seven bands were generated. The number of polymorphic loci varied between 11 and 25 for the AG and TG primers, respectively. This study differentiated the 93 genotypes into six groups with a 0.53 similarity, 0.28 mean heterozygosity (He) for the population, and 0.12±0.02 genetic differentiation coefficient or Fst. A significant level of genetic diversity was evident in the T. cacao genotypes. This resource would benefit selection programs of individual trees or plant breeding programs. The genotypes clustered in a large proportion in accordance with the collection zone. This characteristic was associated with collection zones and along the rivers in the municipality of Tumaco. The RAM technique proved to be a useful tool for the determination of genetic diversity in Theobroma species.
Seis marcadores microsatélites (RAMs) fueron utilizados para caracterizar 93 genotipos de cacao de las zonas productoras del municipio de Tumaco (Colombia). Se generaron 127 bandas. El número de loci polimórfico varió entre 11 y 25 para los cebadores AG y TG, respectivamente. Este estudio diferenció los 93 genotipos en seis grupos con una similaridad de 0,53, una heterocigosidad promedio (He) para la población de 0,28, y un coeficiente de diferenciación genética o Fst de 0,12±0,02. Se evidenció un nivel importante de diversidad genética en los genotipos de T. cacao. Este recurso beneficiaría los programas de selección de árboles individuales o un programa de fitomejoramiento. Los genotipos se agruparon en clusters en gran proporción de acuerdo a su lugar de colecta. Esta característica fue asociada con zonas localizadas en las veredas ubicadas a lo largo de los ríos del municipio de Tumaco. La técnica RAMs demostró ser una herramienta útil para la determinación de la diversidad genética en especies de Theobroma.

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Molecular characterization of 93 genotypes of cocoa (Theobroma cacao L.) with random amplified microsatellites RAMs. (2014). Agronomía Colombiana, 32(3), 315-325. https://doi.org/10.15446/agron.colomb.v32n3.46879

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