Published

2015-05-01

Genetic variability of Papaya ringspot virus isolates in Norte de Santander - Colombia

Variabilidad genética del virus de la mancha anular de la papaya en Norte de Santander - Colombia

DOI:

https://doi.org/10.15446/agron.colomb.v33n2.50095

Keywords:

PRSV, coat protein, recombination, papaya, sequencing (en)
PRSV, proteína de la cubierta, recombinación, papaya, secuenciación (es)

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Authors

  • Giovanni Chaves-Bedoya Universidad Francisco de Paula Santander - Faculty of Basic Sciences - Plantae Research Group
  • Luz Yineth Ortiz-Rojas Universidad Francisco de Paula Santander - Faculty of Basic Sciences - Plantae Research Group
The Papaya ringspot virus (PRSV), a member of the potyvirus that is transmitted by aphids within the Potyviridae family, is the main limiting factor for papaya (Carica papaya L.) and Cucurbits worldwide and causes losses of up to 100%. In this study, we conducted research on the genetic diversity of PRSV isolates collected from two locations in the department of Norte de Santander, Colombia. The analysis was performed by comparing the nucleotide sequences of the region that encode the coat protein (CP) of nine PRSV isolates from the Villa del Rosario location and 12 isolates from the Campo Hermoso location. The analysis included three sequences of the CP of PRSV isolates reported in the Colombian departments of Arauca and Valle del Cauca. The bioinformatic analysis suggested that the PRSV isolates from the locations in Norte de Santander were different from each other, grouping into different phylogenetic groups. Anexistence of recombination events in the coat protein was observed. This is the first study of PRSV genetic variability that has been conducted at the local level in Colombia.

El virus de la mancha anular de la papaya (PRSV), un virus miembro de los potyvirus transmitidos por áfidos dentro de la familia Potyviridae, es el principal factor limitante para los cultivos de papaya (Carica papaya L.) y cucurbitáceas en todo el mundo, llegando a ocasionar pérdidas hasta del 100% de la producción. En este trabajo se realizó el estudio de la diversidad genética de diferentes aislados de PRSV colectados en dos localidades del departamento de Norte de Santander, Colombia. El análisis se realizó comparando las secuencias nucleotídicas de la región que codifica para la proteína de la cubierta (CP) de nueve aislados de la localidad de Villa del Rosario y 12 aislados de la localidad de Campo Hermoso. Para comparación el análisis incluyó tres secuencias de la CP de PRSV de aislados reportados en los departamentos colombianos de Arauca y Valle del Cauca y de otras secuencias informadas en el GenBank. Los análisis bioinformáticos sugieren que los aislados de PRSV de las localidades en Norte de Santander son distintos entre sí agrupándose en grupos filogenéticos diferentes, y se demostró la existencia de eventos de recombinación. Este es el primer estudio de variabilidad genética de PRSV a nivel local en Colombia.

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Chaves-Bedoya, G., & Ortiz-Rojas, L. Y. (2015). Genetic variability of Papaya ringspot virus isolates in Norte de Santander - Colombia. Agronomía Colombiana, 33(2), 184-193. https://doi.org/10.15446/agron.colomb.v33n2.50095

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