Enzymatic hydrolysis of cassava stalks pretreated with the alkaline method

Published

2015-05-01

Enzymatic hydrolysis of cassava stalks pretreated with the alkaline method

Hidrólisis enzimática de tallos de yuca pretratados por el método alcalino

Keywords:

starch digestion, Manihot esculenta Crantz, enzymolysis, lignocellulose (en)
digestión del almidón, Manihot esculenta Crantz, enzimólisis, temperatura, lignocelulosa (es)

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Authors

Hader Castaño P. Politécnico Colombiano Jaime Isaza Cadavid
Juan G. Reales A. Universidad de la Guajira
José Zapata M. Universidad de Antioquia

Abstract (en)
Abstract (es)

This study was developed with the aim of evaluating the pH, enzymatic complex load and temperature effects on the saccharification of pretreated cassava stalks (CS) using the response surface methodology (RSM). The factor levels evaluated were temperature 35-40°C, pH 4.0-5.0 and dose of enzymatic complex Accellerase 1500™ 2.9 - 14.5 FPU/g of substrate. The reducing sugar (RS) response was used. The pH was controlled through the use of hydrochloric acid and sodium hydroxide solutions and the system was shaken orbitally at 120 rpm with a solids loading of 10% w/v. The fitted model showed that the optimal operating conditions were: pH 4.0, 38°C and enzyme dose of 14.5 FPU/g substrate, reaching a sugar concentration of 18.4 g L-1.

Este estudio fue desarrollado con el objetivo de evaluar el efecto del pH, la carga del complejo enzimáticoy la temperatura sobre la sacarificaciónde tallos de yuca pretratados (CS) utilizando la metodologíade superficie de respuesta (RSM). Los niveles de los factores evaluados fueron para la temperatura 35 - 40°C, pH 4,0-5,0 y la dosis del complejo enzimáticoAccellerase 1500™ 2,9-14,5 FPU/g de sustrato. Se utilizó elazúcar reductor (RS) como respuesta. El pH se controlóa través del usode soluciones de ácidoclorhídrico yhidróxidode sodio y el sistema se agitóorbitalmente a 120 rpm con una carga de sólidosde 10% w/v. El modelo ajustado mostró quelas condiciones óptimas de operaciónfueron: pH 4,0, 38°Cy la dosis de enzima de 14,5 FPU/g de sustrato, alcanzando una concentración de azúcarde 18,4 g L-1.

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References

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