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Identification and freedom to operate analysis of potential genes for drought tolerance in maize
Identificación y analisis de libertad de operación en genes potenciales de maíz para la tolerancia a sequía
DOI:
https://doi.org/10.15446/agron.colomb.v35n2.60706Keywords:
propiedad intelectual, cultivos GM, diseño de genes (en)intellectual property, GM crops, gene design (es)
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Drought tolerance is an important character for agricultural crops, particularly corn. Genes confering this feature can be patented, thus hindering their use. From a thorough analysis, three genes (DREB, ZAT10 and CspB) were identified and their sequences were captured in the NCBI database. From these sequences and using free software tools, expression cassettes -including regulatory regions (promoters E35S + Pleader, Ubi-1, rab17; terminators Trub, Tnos)- were designed. Patent searches were conducted in international databases (The Lens and PATENTSCOPE). Four patents and an application were found. In the Colombian national database of the Superintendence of Industry and Commerce (SIC), only the application made through PCT was identified. The claims and nucleotide sequences contained in the application were analyzed and it was found that they do not affect the expression cassettes designed. There is freedom to operate for these constructs and it is possible to continue developing drought-tolerant GM maize lines for the domestic market.
La tolerancia a sequía es un carácter importante para los cultivos agrícolas, en particular para el maíz. Los genes que confieren esta característica pueden estar patentados, dificultando así su uso. A partir de un análisis exhaustivo se identificaron tres de tales genes (DREB, ZAT10 y CspB) y se capturaron las secuencias en las bases de datos del NCBI. A partir de estas secuencias y mediante herramientas de software libre se diseñaron casetes de expresión que incluyeron regiones regulatorias (promotores E35S+pLeader, Ubi-1, rab17; terminadores Trub, Tnos). A continuación se realizaron búsquedas de patentes en bases de datos internacionales (The Lens, y PATENSCOPE). Se encontraron cuatro patentes y una solicitud. En la búsqueda en la base de datos nacional de la Superintendencia de Industria y Comercio, se identificó solo la solicitud realizada por PTC. Analizadas las reivindicaciones y secuencias nucleotídicas contenidas en la solicitud, se encontró que no afectan los casetes de expresión diseñados. Se comprueba la libertad de operación para estos constructos, y la posibilidad de desarrollar líneas. GM tolerantes a sequía para el mercado nacional.
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1. Julian Mora-Oberlaender, Jenny Jiménez-Barreto, Yadira Rodríguez-Abril, Meike Estrada-Arteaga, Alejandro Chaparro-Giraldo. (2022). Cisgenic Crops: Potential and Prospects. Concepts and Strategies in Plant Sciences. , p.89. https://doi.org/10.1007/978-3-031-06628-3_6.
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