Published

2017-05-01

Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon

Respuesta de las plántulas del rábano ( Raphanus sativus L.) a diferentes concentraciones de nitrógeno amoniacal en ausencia y presencia de silicio

DOI:

https://doi.org/10.15446/agron.colomb.v35n2.62772

Keywords:

beneficial element, abiotic stress, nitrogen, nutrient solution, vegetable (en)
elemento benéfico, estrés abiótico, solución nutritiva, hortaliza (es)

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Authors

  • Dilier Olivera Viciedo Universidad Estadual Paulista (UNESP)
  • Renato de Mello Prado Universidad Estadual Paulista (UNESP)
  • Rodolfo Lizcano Toledo Universidad Estadual Paulista (UNESP)
  • Luiz Cláudio Nascimento dos Santos Universidad Estadual Paulista (UNESP)
  • Kolima Peña Calzada Universidad de Sancti Spíritus José Martí Pérez

There are unknown thresholds about the effects of ammonia toxicity in the cultivation of radish and its prejudice is higher in the root than in the aerial part, been the use of silicon an alternative to mitigate this toxicity. The objective was to evaluate the response of radish crop to different concentrations of an ammonium nutrient solution in the absence and presence of silicon under greenhouse conditions. After 30 days of germination were evaluated photosynthesis, green color index, stomatal conductance, transpiration, leaf area, tap root diameter, dry matter accumulation of nitrogen and silicon in shoot parts and roots respectively. Ammonia toxicity in radish decreased photosynthesis, transpiration, and stomatal conductance, having greater prejudice in the dry matter accumulation of root and aerial part, this effect was mitigated with the presence of silicon in the nutrient solution.

Existen dudas sobre los efectos de la toxicidad amoniacal en la fisiología del cultivo del rábano y su perjuicio es mayor en la raíz que en la parte aérea, siendo posible el uso del silicio para mitigar esa toxicidad. Para esto el objetivo fue evaluar la respuesta del cultivo del rábano en función de diferentes concentraciones de NH4+ en la solución nutritiva en ausencia y presencia de silicio. Posterior a los 30 días de la germinación se evaluaron la fotosíntesis, índice de color verde, conductancia estomática, transpiración, área foliar, diámetro de la raíz, materia seca y acúmulos de nitrógeno y silicio respectivamente en la parte aérea y las raíces de las plantas. La toxicidad amoniacal en el rábano disminuyó la fotosíntesis, la transpiración y la conductancia estomática, habiendo mayor perjuicio en el acúmulo de la materia seca de la raíz y la parte aérea, siendo mitigado este efecto con la presencia de silicio en la solución nutritiva.

References

Bataglia, O.C., A.M.C. Furlani, J.P.F. Teixeira, P.R. Furlani, and J.R. Gallo. 1983. Métodos de análise química de plantas. IAC-Boletim Téc. 78. Instituto Agronômico, Campinas, Brazil.

Barreto, R.F., R.M. Prado, A.J.F. Leal, M.J.B. Troleis, G.S. Junior, C.C. Monteiro, and R.F. Carvalho. 2016. Mitigation of ammonium toxicity by silicon in tomato depends on the ammonium concentration. Acta Agric. Scand. Sect. B Soil Plant Sci. 66(6), 483-488. Doi: 10.1080/09064710.2016.1178324.

Bianco, M.S., A.B. Cecilio Filho, and L.B. de Carvalho. 2015. Nutritional status of the cauliflower cultivar 'Verona' grown with omission of out added macronutrients. PloS One 10(4), 1-17. Doi: 10.1371/journal.pone.0123500.

Bittsánszky, A., K. Pilinszky, G. Gyulai, and T. Komives. 2015. Overcoming ammonium toxicity. Plant Sci. 231, 184-190. Doi: 10.1016/j.plantsci.2014.12.005

Bybordi, A. 2010. Influence of NO3:NH4 ratios and silicon on growth, nitrate reductase activity and fatty acid composition of canola under saline conditions. AJAR, 5(15),1984-1992. Doi: 10.5897/AJAR09.064.

Britto, D.T. and H.J. Kronzucker. 2002. NH4 + toxicity in higher plants: a critical review. J. Plant Physiol. 159(6), 567-584. Doi: 10.1078/0176-1617-0774.

Camargo, G.A., L. Consoli, I.C.S. Lellis, J. Mieli, and É.K. Sassaki. 2007. Bebidas naturais de frutas: Perspectivas de mercado, componentes funcionais e nutricionais/natural fruits beverages: Market outlook, functional and nutritional components. Rev. Bioeng. 1(2), 179-205. Doi: 10.18011/bioeng2007v1n2p179-205.

Chen, W., X. Yao, K. Cai, and J. Chen. 2011. Silicon alleviates drought stress of rice plants by improving plant water status, photosynthesis and mineral nutrient absorption. Biol. Trace Elem. Res. 142(1), 67-76. Doi: 10.1007/s12011-010-8742-x.

Feng, J., Q. Shi, X. Wang, M. Wei, F. Yang, and H. Xu. 2010. Silicon supplementation ameliorated the inhibition of photosynthesis and nitrate metabolism by cadmium (Cd) toxicity in Cucumis sativus L. Sci. Hortic. 123 (4), 521-530. Doi: 10.1016/j.scienta.2009.10.013.

Ferreira, D.F. 2011. Sisvar: a computer statistical analysis system. Ciênc. Agrotec. 35(6), 1039-1042. Doi: 10.1590/S1413-70542011000600001.

Filgueira, F.A.R. 2008. Novo manual de olericultura. Agrotecnologia moderna na producao e comercializacao de hortalicas. 3rd ed. Vicosa-MG UFV, São Paulo, Brazil.

Gao Q, Wang and X. Lu. 2014. Effects of exogenous silicon on physiological characteristics of cucumber seedlings under ammonium stress. J Appl Ecol. 25, 1395-1400.

Hachiya, T., C.K. Watanabe, M. Fujimoto, T. Ishikawa, K. Takahara, M. Kawai-Yamada, and K. Noguchi. 2012. Nitrate addition alleviates ammonium toxicity without lessening ammonium accumulation, organic acid depletion and inorganic cation depletion in Arabidopsis thaliana shoots. Plant Cell Physiol. 53(3), 577-591. Doi: 10.1093/pcp/pcs012.

Hoagland, D.R. and D.I. Arnon. 1950. The water-culture method for growing plants without soil. 2nd ed. California Agricultural Experiment Station, Berkeley, USA.

Huang, L., Y. Lu, X. Gao, G. Du, X. Ma, M. Liu, and Y. Chen. 2013. Ammonium-induced oxidative stress on plant growth and antioxidative response of duckweed (Lemna minor L.). Ecol. Eng. 58, 355-362. Doi: 10.1016/j.ecoleng.2013.06.031.

Kochanova, Z., K. Jaskova, B. Sedlakova, and M. Luxova. 2014. Silicon improves salinity tolerance and affects ammonia assimilation in maize roots. Biol. 69, 1164-1171. Doi: 10.2478/s11756-014-0411-7.

Kraska, J.E. and G.A. Breitenbeck. 2010. Simple, robust method for quantifying silicon in plant tissue. Comm. Soil Sci. Plant Anal. 41(17), 2075-2085. Doi: 10.1080/00103624.2010.498537.

Liang, Y., W. Sun, Y.G. Zhu, and P. Christie. 2007. Mechanisms of silicon-mediated alleviation of abiotic stresses in higher plants: a review. Environ. Pollut. 147(2), 422-428. Doi: 10.1016/j.envpol.2006.06.008.

Mateos-Naranjo, E., L. Andrades-Moreno, and A.J. Davy. 2013. Silicon alleviates deleterious effects of high salinity on the halophytic grass Spartina densiflora. Plant Physiol. Biochem. 63, 115-121. Doi: 10.1016/j.plaphy.2012.11.015.

Roosta, H.R. A., Sajjadinia, A. Rahimi, and J.K. Schjoerring. 2009. Responses of cucumber plant to NH4 + and NO3 - nutrition: the relative addition rate technique vs. cultivation at constant nitrogen concentration. Sci. Hortic. 121(4), 397-403. Doi: 10.1016/j.scienta.2009.03.004.

Song, A., P. Li, F. Fan, Z. Li, and Y. Liang. 2014. The Effect of Silicon on Photosynthesis and Expression of Its Relevant Genes in Rice (Oryza sativa L.) under High-Zinc Stress. PLoS One 9(11). Doi: 10.1371/journal.pone.0113782.

Sarasketa, A., M.B. Gonzalez-Moro, C. Gonzalez-Murua, and D. Marino. 2014. Exploring ammonium tolerance in a large panel of Arabidopsis thaliana natural accessions. J. Exp. Bot. Doi: 10.1093/jxb/eru342.

Setien, I., I. Vega-Mas, N. Celestino, M.E. Calleja-Cervantes, C. Gonzalez-Murua, J.M. Estavillo, and M.B. Gonzalez-Moro. 2014. Root phosphoenolpyruvate carboxylase and NAD-malic enzymes activity increase the ammonium-assimilating capacity in tomato. J. Plant Physiol. 171(5), 49-63. Doi: 10.1016/j.jplph.2013.10.021.

Vaculikova, M., M. Vaculik, L. Simkova, I. Fialova, Z. Kochanova, B. Sedlakova, and M. Luxova. 2014. Influence of silicon on maize roots exposed to antimony - Growth and antioxidative response. Plant Physiol. Biochem. 83, 279-284. Doi: 10.1016/j.plaphy.2014.08.014

Zanin, L., A. Zamboni, R. Monte, N. Tomasi, Z. Varanini, S. Cesco, and R. Pinton. 2015. Transcriptomic analysis highlights reciprocal interactions of urea and nitrate for nitrogen acquisition by maize roots. Plant Cell Physiol. 56(3), 532-548. Doi: 10.1093/pcp/pcu202.

Zebarth, B.J., H. Tai, S. Luo, P. Millard, D. De Koeyer, X.Q. Li, and X. Xiong. 2012. Effect of nitrogen form on gene expression in leaf tissue of greenhouse grown potatoes during three stages of growth. Am. J. Potato Res. 89(4), 315-327. Doi: 10.1007/s12230-015-9485-1.

How to Cite

APA

Olivera Viciedo, D., de Mello Prado, R., Lizcano Toledo, R., Nascimento dos Santos, L. C. and Peña Calzada, K. (2017). Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon. Agronomía Colombiana, 35(2), 198–204. https://doi.org/10.15446/agron.colomb.v35n2.62772

ACM

[1]
Olivera Viciedo, D., de Mello Prado, R., Lizcano Toledo, R., Nascimento dos Santos, L.C. and Peña Calzada, K. 2017. Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon. Agronomía Colombiana. 35, 2 (May 2017), 198–204. DOI:https://doi.org/10.15446/agron.colomb.v35n2.62772.

ACS

(1)
Olivera Viciedo, D.; de Mello Prado, R.; Lizcano Toledo, R.; Nascimento dos Santos, L. C.; Peña Calzada, K. Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon. Agron. Colomb. 2017, 35, 198-204.

ABNT

OLIVERA VICIEDO, D.; DE MELLO PRADO, R.; LIZCANO TOLEDO, R.; NASCIMENTO DOS SANTOS, L. C.; PEÑA CALZADA, K. Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon. Agronomía Colombiana, [S. l.], v. 35, n. 2, p. 198–204, 2017. DOI: 10.15446/agron.colomb.v35n2.62772. Disponível em: https://revistas.unal.edu.co/index.php/agrocol/article/view/62772. Acesso em: 27 feb. 2025.

Chicago

Olivera Viciedo, Dilier, Renato de Mello Prado, Rodolfo Lizcano Toledo, Luiz Cláudio Nascimento dos Santos, and Kolima Peña Calzada. 2017. “Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon”. Agronomía Colombiana 35 (2):198-204. https://doi.org/10.15446/agron.colomb.v35n2.62772.

Harvard

Olivera Viciedo, D., de Mello Prado, R., Lizcano Toledo, R., Nascimento dos Santos, L. C. and Peña Calzada, K. (2017) “Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon”, Agronomía Colombiana, 35(2), pp. 198–204. doi: 10.15446/agron.colomb.v35n2.62772.

IEEE

[1]
D. Olivera Viciedo, R. de Mello Prado, R. Lizcano Toledo, L. C. Nascimento dos Santos, and K. Peña Calzada, “Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon”, Agron. Colomb., vol. 35, no. 2, pp. 198–204, May 2017.

MLA

Olivera Viciedo, D., R. de Mello Prado, R. Lizcano Toledo, L. C. Nascimento dos Santos, and K. Peña Calzada. “Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon”. Agronomía Colombiana, vol. 35, no. 2, May 2017, pp. 198-04, doi:10.15446/agron.colomb.v35n2.62772.

Turabian

Olivera Viciedo, Dilier, Renato de Mello Prado, Rodolfo Lizcano Toledo, Luiz Cláudio Nascimento dos Santos, and Kolima Peña Calzada. “Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon”. Agronomía Colombiana 35, no. 2 (May 1, 2017): 198–204. Accessed February 27, 2025. https://revistas.unal.edu.co/index.php/agrocol/article/view/62772.

Vancouver

1.
Olivera Viciedo D, de Mello Prado R, Lizcano Toledo R, Nascimento dos Santos LC, Peña Calzada K. Response of radish seedlings ( Raphanus sativus L.) to different concentrations of ammoniacal nitrogen in absence and presence of silicon. Agron. Colomb. [Internet]. 2017 May 1 [cited 2025 Feb. 27];35(2):198-204. Available from: https://revistas.unal.edu.co/index.php/agrocol/article/view/62772

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