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Molecular characterization of Potato virus Y (PVY) and Potato virus V (PVV) isolates naturally infecting cape gooseberry (Physalis peruviana) in Antioquia, Colombia
Caracterización molecular de aislamientos del virus Y (PVY) y virus V (PVV) de la papa infectando naturalmente uchuva (Physalis peruviana) en Antioquia, Colombia
DOI:
https://doi.org/10.15446/agron.colomb.v36n1.65051Keywords:
genomics, potyviruses, Solanaceae, virus diseases (en)enfermedades virales, genómica, potyvirus, Solanaceae. (es)
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Due to the increase of the international demand for functional fruits, cape gooseberry (Physalis peruviana) has become one of the crops of highest expansion in Colombia and the Andean region of South America. nfortunately, the emergence of fungal and unidentified viral diseases has slowed down the cultivation of cape gooseberry in Colombia and, particularly, in the department of Antioquia. In this work, a next-generation sequencing virome analysis of cape gooseberry plants from eastern Antioquia was performed, using leaves exhibiting symptoms such as mosaics, leaf deformation and greening of veins. The complete genomes of Potato virus Y (PVY) and Potato virus V (PVV) were obtained in the assembled data. The presence of both viruses was confirmed in the samples obtained at two commercial cape gooseberry fields by real time RT-PCR (RT-qPCR) and partial Sanger sequencing of the coat protein (CP). Sequence analysis revealed significant sequence similarity between PVY and PVV isolates infecting P. peruviana to previously identified strains infecting potato (Solanum tuberosum and S. phureja) and tomato (Solanum lycopersicum) in the same geographical region. This study suggests that cape gooseberry could be an alternate host to viruses of other economically important solanaceous crops in the Andean region of South America.
Dado el incremento en la demanda internacional por frutas con caracteristicas funcionales, la uchuva (Physalis peruviana) ha sido uno de los cultivos con mayor expansion en los últimos anos en Colombia y otros paises suramericanos. Desafortunadamente, la emergencia de enfermedades micoticas y virales ha reducido dichos planes de siembra, especialmente en el departamento de Antioquia. En este trabajo, se realizo un análisis de secuenciacion de nueva generacion del viroma asociado a tejidos foliares de plantas de uchuva del oriente antioqueno, con sintomas de mosaicos, deformacion de brotes y verdeamiento de venas. Los genomas completos de los virus Y (PVY) y V (PVV) de la papa fueron obtenidos a partir de las secuencias ensambladas. La presencia de ambos virus fue confirmada en dos cultivos comerciales de uchuva, utilizando RT-PCR en tiempo real y secuenciacion Sanger de una porcion del gen de la capside. Los analisis filogeneticos de dichas secuencias revelaron la existencia de altos niveles de similitud entre los aislamientos de PVY y PVV obtenidos en uchuva y cepas previamente identificadas para esta misma region geográfica en cultivos de papa (Solanum tuberosum y S. phureja) y tomate (Solanum lycopersicum). Este estudio sugiere que las plantas de uchuva pueden servir como hospedantes alternos de virus de importancia economica en cultivos de solanaceas de la región Andina de Suramerica.
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