Published

2019-05-01

Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp.

Transferencia cruzada de marcadores SSR en Setaria sphacelata y Trichloris crinita sp.

DOI:

https://doi.org/10.15446/agron.colomb.v37n2.78785

Keywords:

microsatellites, polymorphism, feed crops, genetic variation, plant genetic resources (en)
microsatélites, polimorfismo, plantas forrajeras, variación genética, recursos genéticos vegetales (es)

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Authors

  • Cecilia Paola Randazzo Instituto de Genética Ewald A. Favret - Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria https://orcid.org/0000-0001-8969-5264
  • Andrea Matilde Ferri Instituto de Genética Ewald A. Favret - Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria
  • Leonela Carabajal Paladino The Pirbright Institute
  • Adriana Noemi Andres Instituto Nacional de Tecnología Agropecuaria - Estación Experimental Agropecuaria
  • Lorena Romina Ingala Instituto de Genética Ewald A. Favret - Centro de Investigación en Ciencias Veterinarias y Agronómicas, Instituto Nacional de Tecnología Agropecuaria

Setaria sphacelata and Trichloris crinita are subtropical forage species that are important for livestock breeding in Argentina. Genomic information is scarce for these species, and there are no molecular markers designed for them; this limits the development of genetic improvement programs. We performed a cross-species transfer of SSR markers from several Poaceae species. In S. sphacelata, 8 SSR markers were transferred from Setaria italica (40% transfer rate), exhibiting 83% polymorphism. Kazungula, Splenda and Narok cultivars were genetically differentiated and the experimental material “Selección INTA” was separated from Narok, from which it was derived. For T. crinita, 19 microsatellites were transferred from 5 Poaceae species (7.3% transfer rate), with 69% polymorphism. The results obtained in this study show the potential of the transferred SSR markers for assessing genetic variation and for expanding the genetic resources available for these species.

Setaria sphacelata y Trichloris crinita son especies forrajeras subtropicales, estratégicas para el desarrollo de la actividad ganadera argentina. Para estas especies, la información genómica es escasa y no existen marcadores moleculares desarrollados en las mismas, por lo cual el desarrollo de programas de mejoramiento genético se ve limitado. En este contexto, realizamos una transferencia de marcadores SSR de varias especies de poáceas. En S. sphacelata, se transfirieron 8 marcadores desarrollados en Setaria italica (tasa de transferencia del 40%), mostrando un 83% de polimorfismo. Los cultivares Kazungula, Splenda y Narok se diferenciaron genéticamente y el material experimental “Selección INTA” se separó de Narok, del cual se deriva. Para T. crinita, se transfirieron 19 microsatélites de 5 especies poáceas (tasa de transferencia del 7.3%), con 69% de polimorfismo. Todos los individuos se pudieron difrenciar genéticamente. Los resultados obtenidos en este trabajo muestran la capacidad de los marcadores SSR transferidos para evaluar la variabilidad genética, expandiendo los recursos genéticos disponibles para estas especies.

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How to Cite

APA

Randazzo, C. P., Ferri, A. M., Carabajal Paladino, L., Andres, A. N. and Ingala, L. R. (2019). Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp. Agronomía Colombiana, 37(2), 112–119. https://doi.org/10.15446/agron.colomb.v37n2.78785

ACM

[1]
Randazzo, C.P., Ferri, A.M., Carabajal Paladino, L., Andres, A.N. and Ingala, L.R. 2019. Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp. Agronomía Colombiana. 37, 2 (May 2019), 112–119. DOI:https://doi.org/10.15446/agron.colomb.v37n2.78785.

ACS

(1)
Randazzo, C. P.; Ferri, A. M.; Carabajal Paladino, L.; Andres, A. N.; Ingala, L. R. Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp. Agron. Colomb. 2019, 37, 112-119.

ABNT

RANDAZZO, C. P.; FERRI, A. M.; CARABAJAL PALADINO, L.; ANDRES, A. N.; INGALA, L. R. Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp. Agronomía Colombiana, [S. l.], v. 37, n. 2, p. 112–119, 2019. DOI: 10.15446/agron.colomb.v37n2.78785. Disponível em: https://revistas.unal.edu.co/index.php/agrocol/article/view/78785. Acesso em: 28 mar. 2024.

Chicago

Randazzo, Cecilia Paola, Andrea Matilde Ferri, Leonela Carabajal Paladino, Adriana Noemi Andres, and Lorena Romina Ingala. 2019. “Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp”. Agronomía Colombiana 37 (2):112-19. https://doi.org/10.15446/agron.colomb.v37n2.78785.

Harvard

Randazzo, C. P., Ferri, A. M., Carabajal Paladino, L., Andres, A. N. and Ingala, L. R. (2019) “Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp”., Agronomía Colombiana, 37(2), pp. 112–119. doi: 10.15446/agron.colomb.v37n2.78785.

IEEE

[1]
C. P. Randazzo, A. M. Ferri, L. Carabajal Paladino, A. N. Andres, and L. R. Ingala, “Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp”., Agron. Colomb., vol. 37, no. 2, pp. 112–119, May 2019.

MLA

Randazzo, C. P., A. M. Ferri, L. Carabajal Paladino, A. N. Andres, and L. R. Ingala. “Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp”. Agronomía Colombiana, vol. 37, no. 2, May 2019, pp. 112-9, doi:10.15446/agron.colomb.v37n2.78785.

Turabian

Randazzo, Cecilia Paola, Andrea Matilde Ferri, Leonela Carabajal Paladino, Adriana Noemi Andres, and Lorena Romina Ingala. “Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp”. Agronomía Colombiana 37, no. 2 (May 1, 2019): 112–119. Accessed March 28, 2024. https://revistas.unal.edu.co/index.php/agrocol/article/view/78785.

Vancouver

1.
Randazzo CP, Ferri AM, Carabajal Paladino L, Andres AN, Ingala LR. Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp. Agron. Colomb. [Internet]. 2019 May 1 [cited 2024 Mar. 28];37(2):112-9. Available from: https://revistas.unal.edu.co/index.php/agrocol/article/view/78785

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