Cross-species transfer of SSR markers in Setaria sphacelata and Trichloris crinita sp.
Transferencia cruzada de marcadores SSR en Setaria sphacelata y Trichloris crinita sp.
DOI:
https://doi.org/10.15446/agron.colomb.v37n2.78785Keywords:
microsatellites, polymorphism, feed crops, genetic variation, plant genetic resources (en)microsatélites, polimorfismo, plantas forrajeras, variación genética, recursos genéticos vegetales (es)
Downloads
Setaria sphacelata and Trichloris crinita are subtropical forage species that are important for livestock breeding in Argentina. Genomic information is scarce for these species, and there are no molecular markers designed for them; this limits the development of genetic improvement programs. We performed a cross-species transfer of SSR markers from several Poaceae species. In S. sphacelata, 8 SSR markers were transferred from Setaria italica (40% transfer rate), exhibiting 83% polymorphism. Kazungula, Splenda and Narok cultivars were genetically differentiated and the experimental material “Selección INTA” was separated from Narok, from which it was derived. For T. crinita, 19 microsatellites were transferred from 5 Poaceae species (7.3% transfer rate), with 69% polymorphism. The results obtained in this study show the potential of the transferred SSR markers for assessing genetic variation and for expanding the genetic resources available for these species.
Setaria sphacelata y Trichloris crinita son especies forrajeras subtropicales, estratégicas para el desarrollo de la actividad ganadera argentina. Para estas especies, la información genómica es escasa y no existen marcadores moleculares desarrollados en las mismas, por lo cual el desarrollo de programas de mejoramiento genético se ve limitado. En este contexto, realizamos una transferencia de marcadores SSR de varias especies de poáceas. En S. sphacelata, se transfirieron 8 marcadores desarrollados en Setaria italica (tasa de transferencia del 40%), mostrando un 83% de polimorfismo. Los cultivares Kazungula, Splenda y Narok se diferenciaron genéticamente y el material experimental “Selección INTA” se separó de Narok, del cual se deriva. Para T. crinita, se transfirieron 19 microsatélites de 5 especies poáceas (tasa de transferencia del 7.3%), con 69% de polimorfismo. Todos los individuos se pudieron difrenciar genéticamente. Los resultados obtenidos en este trabajo muestran la capacidad de los marcadores SSR transferidos para evaluar la variabilidad genética, expandiendo los recursos genéticos disponibles para estas especies.
References
Aronson, J.A. 1989. HALOPH: a data base of salt tolerant plants of the world. Office of Arid Land Studies, University of Arizona. Tucson, USA.
Arya, L., M. Verma, V.K. Gupta, and J.L. Karihaloo. 2009. Development of EST SSRs in Finger Millet (Eleusine coracana ssp coracana) and their transferability to Pearl Millet (Pennisetum glaucum). J. Plant Biochem. Biotech. 18 (1), 97-100. Doi: 10.1007/BF03263303
Balzarini, M.G. and J.A. Di Rienzo. 2016. InfoGen version 2016. URL: http://www.info-gen.com.ar.
Barbará, T., C. Palma-Silva, G.M. Paggi, F. Bered, M.F. Fay, and C. Lexer. 2007. Cross-species transfer of nuclear microsatellite markers: potential and limitations. Mol. Ecol. 16, 3759-3767. Doi: 10.1111/j.1365-294X.2007.03439.x
Borrajo, C.I. and R. Pizzio. 2006. Manual de producción y utilización de Setaria. Libreta técnica. Proyecto Ganadero de Corrientes. EEA Mercedes, INTA. Corrientes, Argentina.
Borrajo, C.I., P. Barbera, and D. Bendesky. 2009. Comportamiento de variedades de Setaria sphacelata. Rev. Arg. Prod. Anim. 29 (1), 508-510.
Botstein, D., R.L. White, M. Skolnick, and R.W. Davis. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am. J. Hum. Genet. 32 (3), 314-331.
Burghi, V., R. Avila, P. Barbera, L. Blanco, J.P. De Battista, C. Frasinelli, K. Frigerio, L. Gandara, M.C. Goldfarb, S. Griffa, K. Grunberg, K. Leal, C. Kunst, S. María Lacorte, A. Lauric, L. Martínez Calsina, G. Mc. Lean, F. Nenning, J. Otondo, and J. Veneciano. 2014. Gramíneas forrajeras para el subtrópico y el semiárido central de la Argentina. Forage grasses for the subtropics and central semiarid Argentina.URL: https://bit.ly/2P5L194 (accessed 31 January 2019).
Cano, E. 1988. Descripción de las especies más importantes. Tomo I. pp. 425. In: Cano, E. and J.B. Nazar Anchorena (eds). Pastizales naturales de La Pampa. Editorial AACREA. La Pampa, Argentina.
Cavagnaro, P., J. Cavagnaro, J. Lemes, R. Masuelli, and C. Passera. 2006. Genetic diversity among varieties of the native forage grass Trichloris crinita based on AFLP markers, morphological characters, and quantitative agronomic traits. Genome 49, 906-918. Doi: 10.1139/g06-060
Chandra, A. and K.K. Tiwari. 2010. Isolation and characterization of microsatellite markers from guineagrass (Panicum maximum Jacq.) for genetic diversity estimate and crossspecies amplification. Plant Breed. 129, 120-124. Doi: 10.1111/j.1439-0523.2009.01651.x
Dalmaso, A.D. 1994. Fenología de cinco gramíneas nativas de interés forrajero. Multiequina 3, 9-24.
Dellaporta, S.L., J. Wood, and J.B. Hicks. 1983. A plant DNA mini preparation: Version II. Plant Mol. Biol. Rep. 1 (4), 19-21. Doi: 10.1007/BF02712670
Dida, M., M. Srinivasachary, S. Ramakrishnan, J.L. Bennetzen, M.D. Gale, and K.M. Devos. 2007. The genetic map of Finger millet, Eleusine coracana. Theor. Appl. Genet. 114, 321-332. Doi: 10.1007/s00122-006-0435-7
Ebina, M., K. Kouki, S. Tsuruta, R. Akashi, T. Yamamoto, M. Takahara, M. Inafuku, K. Okumura, H. Nakagawa, and K. Nakajima. 2007. Genetic relationship estimation in guineagrass (Panicum maximum Jacq.) assessed on the basis of simple sequence repeat markers. Grassl. Sci. 53, 155-164. Doi: 10.1111/j.1744-697X.2007.00086.x
Fedorov, A.A. 1969. Chromosome numbers of flowering plants. Academy of Sciences of the USSR. Nauka, Leningrad, USSR.
Greco, S. and J. Cavagnaro. 2003. Effects of drought in biomass production and allocation in three varieties of Trichloris crinita P. (Poaceae) a forage grass from the arid Monte region of Argentina. Plant Ecol. 164, 125-135. Doi: 10.1023/A:1021217614767
Gupta, S., K. Kumari, P.P. Sahu, S. Vidapu, and M. Prasad. 2012. Sequence-based novel genomic microsatellite markers for robust genotyping purposes in foxtail millet [Setaria italica (L.) P. Beauv.]. Plant Cell Rep. 31, 323-337. Doi: 10.1007/s00299-011-1168-x
Gupta, S., K. Kumari, M. Muthamilarasan, A. Subramanian, and M. Prasad. 2013. Development and utilization of novel SSRs in foxtail millet [Setaria italica (L.) P. Beauv.]. Plant Breed. 132 (4), 367-374. Doi: 10.1111/pbr.12070
Hacker, J.B. 1991. Seed production potential in bred populations and cultivars of Setaria sphacelata. Trop. Grasslands 25, 253-261.
Hacker, J.B. and R.J. Jones. 1969. The Setaria sphacelata complex - A review. Trop. Grasslands 3(1), 13-25.
Hacker, J.B. and R.L. Cuany. 1997. Genetic variation in seed production and its components in four cultivars of the pasture grass Setaria sphacelata. Euphytica 93, 271-282. Doi: 10.1023/A:1002932432121
Jank, L. and J.B. Hacker. 2004. Setaria. pp. 785-807. In: Moser L.E., B.L. Burson, and L.E. Sollenberger eds. Warm-season (C4) grasses. Agronomy Monography 45. Madison, USA.
Jank, L., K.H. Quesenberry, L.E. Sollenberger, D.S. Wofford, and P.M. Lyrene. 2007. Selection of morphological traits to improve forage characteristics of Setaria sphacelata grown in Florida. New Zeal. J. Agr. Res. 50(1), 73-83. Doi: 10.1080/00288230709510284
Jessup, R.W. 2005. Molecular tools for marker-assisted breeding of
buffelgrass. PhD thesis, Texas A&M University. Texas, USA.
Jia, X.P., Y.S. Shi, Y.C. Song, G.Y. Wang, and T.Y. Wang. 2007. Development
of EST-SSR in foxtail millet (Setaria italica). Genet. Resour. Crop Ev. 54, 233-236. Doi: 10.1007/s10722-006-9139-8
Jones, E.S., M.P. Dupal, R. Kölliker, M.C. Drayton, and J.W. Forster. 2001. Development and characterization of simple sequence repeat (SSR) markers for perennial ryegrass (Lolium perenne L.). Theor. Appl. Genet, 102(2-3), 405-415. Doi: 10.1007/s001220051661
Kozub, P.C., K. Barboza, F. Galdeano, C.L. Quarin, J.B. Cavagnaro, and P.F. Cavagnaro. 2017. Reproductive biology of the native forage grass Trichloris crinita (Poaceae, Chloridoideae). Plant Biol. 19(3), 444-453. Doi: 10.1111/plb.12549
Kozub, P.C., K. Barboza, and J.B. Cavagnaro. 2018. Development and characterization of SSR markers for Trichloris crinita using sequence data from related grass species. Rev. FCA. UNCUYO. 50(1), 1-16.
Kumari, K., M. Muthamilarasan, G. Misra, S. Gupta, A. Subramanian, S.K. Parida, D. Chattopadhyay, and M. Prasad. 2013. Development of eSSR-markers in Setaria italica and their applicability in studying genetic diversity, cross-transferability and comparative mapping in millet and non-millet species. PLoS ONE. 8(6), e67742. Doi: 10.1371/journal.pone.0067742
Le Thierry d’Ennequin, M., O. Panaud, S. Brown, S. Siljak-Yakovlev,
and A. Sarr. 1998. First evaluation of nuclear DNA content in Setaria genus by flow cytometry. J. Hered. 89(6), 556-559. Doi: 10.1093/jhered/89.6.556
Li, Y., J. Jia, Y. Wang, and S. Wu. 1998. Intraspecific and interspecific variation in Setaria revealed by RAPD analysis. Genet. Resour. Crop Ev. 45(3), 279-285. Doi: 10.1023/A:1008600123509
Nicora, E.G. and Z.E. Rúgolo de Agrasar. 1987. Los géneros de gramíneas de América Austral: Argentina, Chile, Uruguay y áreas limítrofes de Bolivia, Paraguay y Brasil. Editorial Hemisferio Sur, Buenos Aires.
Pandey, G., G. Misra, K. Kumari, S. Gupta, S.K. Parida, D. Chattopadhyay, and M. Manoj Prasad. 2013. Genome-wide development and use of microsatellite markers for large-scale genotyping applications in foxtail millet [Setaria italica (L.)]. DNA Res. 20(2), 197-207. Doi: 10.1093/dnares/dst002
Peakall, R. and P.E. Smouse. 2006. GENALEX 6: genetic analysis in Excel.
Population genetic software for teaching and research. Mol. Ecol. Notes 6(1), 288-295. Doi: 10.1111/j.1471-8286.2005.01155.x
Pensiero, J.F. 1999. Las especies sudamericanas del género Setaria (Poaceae, Paniceae). Darwiniana 37, 37-151.
Pérez de la Torre, M., P. Zirilli, M. Rodríguez, and A. Escandón. 2008. Análisis de la variabilidad genética utilizando marcadores moleculares en el género Mecardonia. Proceedings of the 4th Congreso Argentino de Floricultura y Plantas Ornamentales and the 10th Jornadas Nacionales de Floricultura. Corrientes, Argentina.
Quiroga, E.R., R.A. Golluscio, L.J. Blanco, and R.J. Fernandez. 2010. Aridity and grazing as convergent selective forces: an experiment with an Arid Chaco bunchgrass. Ecol. Appl. 20(7), 1876-1889. Doi: 10.1890/09-0641.1
Ramakrishnan, A., S.E. Meyer, J. Waters, M.R. Stevens, C.E. Coleman, and D.J. Fairbanks. 2004. Correlation between molecular markers and adaptively significant genetic variation in Bromus tectorum (Poaceae), an inbreeding annual grass. Am. J. Bot. 91, 797-803.
Röder, M.S., V. Korzun, K. Wendehake, J. Plaschke, M.H. Tixier, P. Leroy, and M.W. Ganal. 1998. A microsatellite map of wheat. Genetics 149, 2007-2023.
Saha, M.C., J.C. Zwonitzer, A.A. Hopkins, and M.A.R. Mian. 2003. A molecular linkage map of a tall fescue population segregating for forage quality traits. In: Molecular breeding of forage and turf. Proceedings of the 3rd International Symposium. Dallas, USA.
Saha, M.C., J.D. Cooper, M.A. Mian, K. Chekhovskiy, and G.D. May. 2006. Tall fescue genomic SSR markers: development and transferability across multiple grass species. Theor. Appl. Genet. 113(8), 1449-58. Doi: 10.1007/s00122-006-0391-2
Snow, N. and P.M. Peterson. 2012. Nomenclatural notes on Dinebra, Diplachne, Disakisperma, and Leptochloa (Poaceae: Chloridoideae). Phytoneuron 71, 1-2.
Wang, M.L., N. Barkley, J. Yu, R. Dean, M. Newman, M. Zorreéis, and G. Pederson. 2005. Transfer of simple sequence repeat (SSR) markers from major cereal crops to minor grass species for germplasm characterization and evaluation. Plant Genet. Resour. 3(1), 45-57. Doi: 10.1079/PGR200461
How to Cite
APA
ACM
ACS
ABNT
Chicago
Harvard
IEEE
MLA
Turabian
Vancouver
Download Citation
CrossRef Cited-by
1. Vikas C. Tyagi, Tejveer Singh, Nilamani Dikshit, Sultan Singh, Maneet Rana, Rahul Kaldate, Prabhu Govindaswamy, Hanamant M. Halli, Avijit Ghosh, Rajesh Kumar Singhal, Manjanagouda S. Sannagoudar. (2023). Molecular Interventions for Developing Climate-Smart Crops: A Forage Perspective. , p.3. https://doi.org/10.1007/978-981-99-1858-4_1.
Dimensions
PlumX
Article abstract page views
Downloads
License
Copyright (c) 2019 Agronomía Colombiana
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
© Centro Editorial de la Facultad de Ciencias Agrarias, Universidad Nacional de Colombia
Reproduction and quotation of material appearing in the journal is authorized provided the following are explicitly indicated: journal name, author(s) name, year, volume, issue and pages of the source. The ideas and observations recorded by the authors are their own and do not necessarily represent the views and policies of the Universidad Nacional de Colombia. Mention of products or commercial firms in the journal does not constitute a recommendation or endorsement on the part of the Universidad Nacional de Colombia; furthermore, the use of such products should comply with the product label recommendations.
The Creative Commons license used by Agronomia Colombiana journal is: Attribution - NonCommercial - ShareAlike (by-nc-sa)
Agronomia Colombiana by Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia is licensed under a Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional License.
Creado a partir de la obra en http://revistas.unal.edu.co/index.php/agrocol/.