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Detection and molecular characterization of the cucumber mosaic virus in chili pepper (Capsicum spp. L.) crops
Detección y caracterización molecular del virus del mosaico del pepino en cultivos de ají (Capsicum spp. L.)
DOI:
https://doi.org/10.15446/agron.colomb.v38n2.82975Keywords:
Cucumovirus, diagnostics, habanero, PCR, Solanaceae, tabasco (en)Cucumovirus, diagnóstico, habanero, PCR, Solanaceae, tabasco (es)
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The chili pepper (Capsicum spp. L.) is a vegetable of economic importance that has been affected worldwide by the cucumber mosaic virus (CMV), a pathogen that causes a devastating disease in this crop. The aim of this research was the detection and characterization of CMV in chili pepper crops in Valle del Cauca, Colombia. Leaves of three chili pepper varieties (tabasco, cayenne and habanero) with viral symptoms were collected in four municipalities of Valle del Cauca. Total RNA was purified and a fragment of capsid protein (CP) from CMV was amplified by RT‑PCR. Then, it was sequenced and bioinformatically analyzed, and from these sequences, specific primers were designed. From 71 chili pepper samples collected in Palmira, Yumbo, Vijes and Yotoco, 37 were positive for CMV (52.1%). The CMV chili pepper sequence analysis showed that they had their highest identity (98.5%) with a CMV isolated from bananas in Ecuador. Specific primers designed for CMV chili pepper showed greater sensitivity for detecting this virus (64.7% vs. 52.1%). The CMV chili pepper CP analysis indicated that it could be transmitted by the species Aphis gossypii. This r the first time, the molecular characterization of CMV in three chili pepper varieties.
El ají (Capsicum spp. L.) es una hortaliza de importancia económica que se ha visto afectada por el virus del mosaico del pepino (cucumber mosaic virus ‑ CMV), un patógeno que ocasiona una enfermedad devastadora para este cultivo a nivel mundial. El objetivo de la investigación fue la detección y caracterización molecular de CMV en cultivos de ají en el Valle del Cauca, Colombia. Se recolectaron hojas de tres variedades de ají (tabasco, cayena y habanero) con síntomas virales en cuatro municipios del Valle del Cauca. Se purificó su RNA total y se amplificó un fragmento del gen de la proteína de la cápside (CP) de CMV por RT‑PCR. Después se secuenció, se analizó bioinformáticamente y a partir de estas secuencias se diseñaron primers específicos. De 71 muestras de ají colectadas en Palmira, Yumbo, Vijes y Yotoco, 37 resultaron positivas para CMV (52,1%). El análisis de las secuencias de CMV ají mostró que presentaban su mayor identidad (98,5%) con un CMV aislado de banano en Ecuador. Los primers específicos diseñados para CMV ají mostraron mayor sensibilidad para detectar este virus (64,7% vs. 52,1%). El análisis de CP de CMV ají indicó que este virus podría ser transmitido por la especie Aphis gossypii. Este estudio reporta, por primera vez, l ción molecular de CMV en tres variedades de ají.
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