Correo Electrónico
DNINFOA - SIA
Bibliotecas
Convocatorias
Identidad U.N.
Published
Effect of a mix of oligogalacturonides on symbiotic nitrogen fixation in common bean
Efecto de una mezcla de oligogalacturónidos en la fijación simbiótica del nitrógeno en frijol común
DOI:
https://doi.org/10.15446/agron.colomb.v39n1.92081Keywords:
oligosaccharides, symbiosis, Phaseolus vulgaris L., Rhizobium (en)oligosacáridos, simbiosis, Phaseolus vulgaris L., Rhizobium (es)
Downloads
The objective of this study was to evaluate the effect of a mix of pectic oligosaccharides in the common bean-rhizobia symbiosis. Cuba Cueto-25-9-N bean seeds were inoculated with Rhizobium tropici CIAT 899 and treated with a mix of oligogalacturonides, either by application to the seed (10 mg L-1, 1 ml per seed) at sowing and inoculation or by foliar spray (5 or 100 mg L-1, 1.5 ml per plant) to plants with two trefoils. Plant growth, nodulation, nitrogenase activity, and the gene expression of glutamine synthetase and glutamate synthase in nodules were evaluated in inoculated plants and oligogalacturonide-treated inoculated plants at 18 d post-inoculation. The oligogalacturonide-treated plants showed increases in shoot and root growth and number of nodules. Regarding nodule function, the oligogalacturonides increased the nitrogenase activity and the expression level of the gene coding for NADH-dependent glutamate synthase. The positive effects of oligogalacturonides resulted in higher effectiveness of the symbiotic nitrogen fixation. The application of oligogalacturonides can offer an alternative to increasing the symbiotic nitrogen fixation in common bean plants.
El objetivo de este estudio fue evaluar el efecto de una mezcla de oligosacáridos pécticos en la simbiosis frijol común-rizobios. Las semillas de frijol Cuba Cueto-25-9-N se inocularon con Rhizobium tropici CIAT 899 y se trataron con una mezcla de oligogalacturónidos por aplicación a la semilla (10 mg L-1, 1 ml por semilla) en el momento de la siembra e inoculación o por aspersión foliar (5 o 100 mg L-1, 1.5 ml por planta) a plantas con dos trifoliolos. A los 18 d después de la inoculación, se evaluó el crecimiento de las plantas, la nodulación, la actividad nitrogenasa y la expresión génica de la glutamina sintetasa y la glutamato sintasa en nódulos de plantas inoculadas y en plantas inoculadas y tratadas con oligogalacturónidos. Las plantas tratadas con oligogalacturónidos mostraron aumentos en el crecimiento del vástago y raíces y en el número de nódulos. En cuanto a la función de los nódulos, los oligogalacturónidos aumentaron la actividad nitrogenasa y el nivel de expresión del gen que codifica la glutamato sintasa dependiente de NADH. Los efectos positivos de los oligogalacturónidos resultaron en una mayor efectividad de la fijación simbiótica del nitrógeno. La aplicación de oligogalacturónidos puede ofrecer una alternativa para incrementar la fijación simbiótica del nitrógeno en plantas de frijol común.
References
Barraza, A., Coss-Navarrete, E. L., Vizuet-de-Rueda, J. C., Martínez-Aguilar, K., Hernández-Chávez, J. L., Ordaz-Ortiz, J. J., Winkler, R., Tiessena, A., & Alvarez-Venegas, R. (2018). Down-regulation of PvTRX1h increases nodule number and affects auxin, starch, and metabolic fingerprints in the common bean (Phaseolus vulgaris L.). Plant Science, 274, 45–58. https://doi.org/10.1016/j.plantsci.2018.05.006
Bensmihen, S. (2015). Hormonal Control of Lateral Root and Nodule Development in Legumes. Plants, 4(3), 523-547. https://doi.org/10.3390/plants4030523
Broughton, W. J., Hernandez, G., Blair, M., Beebe, S., Gepts, P., & Vanderleyden, J. (2003). Beans (Phaseolus spp.) - model food legumes. Plant and Soil, 252, 55–128. https://doi.org/0.1023/A:1024146710611
Cabrera, J. C., Gómez, R., Diosdado, E., Hormaza, J., Iglesias, R., Gutiérrez, A., & González, S. (2003). Procedure to obtain a pectic oligosaccharide mixture with plant rooting activity. Cuban patent no. 22859, Resolution 155/2003.
Cabrera, J. C., Wégria, G., Onderwater, R. C. A., González, G., Nápoles, M. C., Falcón-Rodríguez, A. B., Costales, D., Rogers, H. J., Diosdado, E., González, S., Cabrera, G., González, L., & Wattiez, R. (2013). Practical use of oligosaccharins in agriculture. Acta Horticulturae, 1009, 195–212. https://doi.org/10.17660/ActaHortic.2013.1009.24
Camejo, D., Martí, M. C., Jiménez, A., Cabrera, J. C., Olmos, E., & Sevilla, F. (2011). Effect of oligogalacturonides on rootlength, extracellular alkalinization and O2− -accumulation in alfalfa. Journal of Plant Physiology, 168(6), 566–575. https://doi.org/10.1016/j.jplph.2010.09.012
Chalk, P. M., He, J. Z., Peoples, M. B., & Chen, D. (2017). 15N2 as a tracer of biological N2 fixation: a 75-year retrospective. Soil Biology and Biochemistry, 106, 36–50. https://doi.org/10.1016/j.soilbio.2016.12.010
Cordoba, E., Shishkova, S., Vance, C. P., & Hernández, G. (2003). Antisense inhibition of NADH glutamate synthase impairs carbon/nitrogen assimilation in nodules of alfalfa (Medicago sativa L.). The Plant Journal, 33(6), 1037–1049. https://doi.org/10.1046/j.1365-313X.2003.01686.x
Farid, M., Earl, H. J., Pauls, K. P., & Navabi, A. (2017). Response to selection for improved nitrogen fixation in common bean (Phaseolus vulgaris L.). Euphytica, 213, Article 99. https://doi.org/10.1007/s10681-017-1885-5
Haag, A. F., Arnold, M. F. F., Myka, K. K., Kerscher, B., Dall’Angelo, S., Zanda, M., Mergaert, P., & Ferguson, G. P. (2013). Molecular insights into bacteroid development during Rhizobium-legume symbiosis. FEMS Microbiology Reviews, 37(3), 364–383. https://doi.org/10.1111/1574-6976.12003
Hardy, R. W. F., Holsten, R. D., Jackson, E. K., & Burns, R. C. (1968). The acetylene-ethylene assay for N2 fixation: laboratory and field evaluation. Plant Physiology, 43(8), 1185–1207. https://doi.org/10.1104/pp.43.8.1185
Hernández, G., Ramírez, M., Valdés-López, O., Tesfaye, M., Graham, M. A., Czechowski, T., Schlereth, A., Wandrey, M., Erban, A., Cheung, F., Wu, H. C., Lara, M., Town, C. D., Kopka, J., Udvardi, M. K., & Vance, C. P. (2007). Phosphorus stress in common bean: root transcript and metabolic responses. Plant Physiology, 144(2), 752–767. https://doi.org/10.1104/pp.107.096958
Hungria, M., Nogueira, M. A., & Araujo, R. S. (2013). Co-inoculation of soybeans and common beans with rhizobia and azospirilla: strategies to improve sustainability. Biology and Fertility of Soils, 49, 791–801. https://doi.org/10.1007/s00374-012-0771-5
Izquierdo, H., Diosdado, E., González Cepero, M. C., Núñez, M. C., Cabrera, J. C., Hernández, R. M., González, J. L., Hernández, M. M., Héctor, E. F., Gómez, R., Proenza, R., & Velásquez, M. (2016). Contributions to knowledge of the functioning of national bioestimulators in plant biotechnology processes. Biotecnología Aplicada, 33(3), 3511–3516.
Jesus, E. D. C., Leite, R. D. A., Bastos, R. D. A., Aragão, O. O. D. S., & Araújo, A. P. (2018). Co-inoculation of Bradyrhizobium stimulates the symbiosis efficiency of Rhizobium with common bean. Plant and Soil, 425, 201–215. https://doi.org/10.1007/s11104-017-3541-1
Kohlen, W., Ng, J. L. P., Deinum, E. E., & Mathesius, U. (2018). Auxin transport, metabolism, and signaling during nodule initiation: indeterminate and determinate nodules. Journal of Experimental Botany, 69(2), 229–244. https://doi.org/10.1093/jxb/erx308
Kuypers, M. M. M., Marchant, H. K., & Kartal, B. (2018). The microbial nitrogen-cycling network. Nature Reviews Microbiology, 16, 263–276. https://doi.org/10.1038/nrmicro.2018.9
Lara-Acosta, D., Costales-Menéndez, D., Nápoles-García, M. C., & Falcón-Rodríguez, A. (2019). Pectimorf® and Azofert-F® in the growth of bean plants (Phaseolus vulgaris L.). Cultivos Tropicales, 40(4), Article e05.
Martínez-Romero, E., Segovia, L., Mercante, F. M., Franco, A. A., Graham, P., & Pardo, M. A. (1991). Rhizobium tropici, a novel species nodulating Phaseolus vulgaris L. beans and Leucaena sp. trees. International Journal of Systematic Bacteriology, 41(3), 417–426. https://doi.org/10.1099/00207713-41-3-417
Oliveira, D. P., Figueiredo, M. A., Soares, B. L., Teixeira, O. H. S., Martins, F. A. D., Rufini, M., Chain, C. P., Reis, R. P., Morais, A. R., Moreira, F. M. S., & Andrade, M. J. B. (2017). Acid tolerant Rhizobium strains contribute to increasing the yield and profitability of common bean in tropical soils. Journal of Soil Science and Plant Nutrition, 17(4), 922–934. https://doi.org/10.4067/S0718-95162017000400007
Ormeño-Orrillo, E., Menna, P., Almeida, L. G. P., Ollero, F. J., Nicolás, M. F., Rodrigues, E. P., Nakatani, A. S., Batista, J. S. S., Chueire, L. M. O., Souza, R. C., Vasconcelos, A. T. R., Megías, M., Hungria, M., & Martínez-Romero, E. (2012). Genomic basis of broad host range and environmental adaptability of Rhizobium tropici CIAT 899 and Rhizobium sp. PRF 81 which are used in inoculants for common bean (Phaseolus vulgaris L.). BMC Genomics, 13, Article 735. https://doi.org/10.1186/1471-2164-13-735
Polania, J., Poschenrieder, C., Rao, I., & Beebe, S. (2016). Estimation of phenotypic variability in symbiotic nitrogen fixation ability of common bean under drought stress using 15N natural abundance in grain. European Journal of Agronomy, 79, 66–73. https://doi.org/10.1016/j.eja.2016.05.014
Ramírez, M., Guillén, G., Fuentes, S. I., Iñiguez, L. P., Aparicio-Fabre, R., Zamorano-Sánchez, D., Encarnación-Guevara, S., Panzeri, D., Castiglioni, B., Cremonesi, P., Strozzi, F., Stella, A., Girard, L., Sparvoli, F., & Hernández, G. (2013). Transcript profiling of common bean nodules subjected to oxidative stress. Physiologia Plantarum, 149(3), 389–407. https://doi.org/10.1111/ppl.12040
Ridley, B. L., O’Neill, M. A., & Mohnen, D. (2001). Pectins: structure, biosynthesis, and oligogalacturonide-related signaling. Phytochemistry, 57(6), 929–967. https://doi.org/10.1016/S0031-9422(01)00113-3
Roy, S., Liu, W., Nandety, R. S., Crook, A., Mysore, K. S., Pislariu, C. I., Frugoli, J., Dickstein, R., & Udvardi, M. K. (2020). Celebrating 20 years of genetic discoveries in legume nodulation and symbiotic nitrogen fixation. The Plant Cell, 32(1), 15–41. https://doi.org/10.1105/tpc.19.00279
Savatin, D. V., Ferrari, S., Sicilia, F., & De Lorenzo, G. (2011). Oligogalacturonide-auxin antagonism does not require posttranscriptional gene silencing or stabilization of auxin response repressors in Arabidopsis. Plant Physiology, 157(3), 1163–1174. https://doi.org/10.1104/pp.111.184663
Summerfield, R. J., Huxley, P. A., & Minchin, F. R. (1977). Plant husbandry and management techniques for growing grain legumes under simulated tropical conditions in controlled environments. Experimental Agriculture, 13(1), 81–92. https://doi.org/10.1017/S0014479700007638
Temple, S. J., Vance, C. P., & Gantt, J. S. (1998). Glutamate synthase and nitrogen assimilation. Trends in Plant Science, 3(2), 51–56. https://doi.org/10.1016/S1360-1385(97)01159-X
Xin, C., Qing-We, Y., Jia-Lin, S., Shuang, X., Fu-Chun, X., & Ya-Jun, C. (2014). Research progress on nitrogen use and plant growth. Journal of Northeast Agricultural University, 21(2), 68–74. https://doi.org/10.1016/S1006-8104(14)60036-2
How to Cite
APA
ACM
ACS
ABNT
Chicago
Harvard
IEEE
MLA
Turabian
Vancouver
Download Citation
CrossRef Cited-by
1. Ariel López Duany, Juan Carlos Marín Arias, Elsy María Rivero González. (2024). Predicción del rendimiento agrícola en el cultivo de la habichuela . Revista Científica Sapientia Technological, 5(1), p.56. https://doi.org/10.58515/021RSPT.
Dimensions
PlumX
Article abstract page views
Downloads
License
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
© Centro Editorial de la Facultad de Ciencias Agrarias, Universidad Nacional de Colombia
Reproduction and quotation of material appearing in the journal is authorized provided the following are explicitly indicated: journal name, author(s) name, year, volume, issue and pages of the source. The ideas and observations recorded by the authors are their own and do not necessarily represent the views and policies of the Universidad Nacional de Colombia. Mention of products or commercial firms in the journal does not constitute a recommendation or endorsement on the part of the Universidad Nacional de Colombia; furthermore, the use of such products should comply with the product label recommendations.
The Creative Commons license used by Agronomia Colombiana journal is: Attribution - NonCommercial - ShareAlike (by-nc-sa)
Agronomia Colombiana by Centro Editorial of Facultad de Ciencias Agrarias, Universidad Nacional de Colombia is licensed under a Creative Commons Reconocimiento-NoComercial-CompartirIgual 4.0 Internacional License.
Creado a partir de la obra en http://revistas.unal.edu.co/index.php/agrocol/.
