Published

2022-04-30

Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg

La pudrición basal en clavel (Dianthus caryophyllus L.) es causada por Fusarium verticillioides (Sacc.) Nirenberg

DOI:

https://doi.org/10.15446/agron.colomb.v40n1.99009

Keywords:

Fusarium oxysporum, Fusarium roseum, morphological characterization, vascular wilt (en)
Fusarium oxysporum, Fusarium roseum, caracterización morfológica, marchitez vascular (es)

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Authors

  • Juan José Filgueira Duarte Universidad Militar Nueva Granada - Faculty of Basic Sciences - Laboratory of Vegetal Biotechnology - Cajicá/Zipaquirá, Cundinamarca - Colombia https://orcid.org/0000-0002-9814-6352
  • Cindy Melissa Rincón-Sandoval Universidad Militar Nueva Granada - Faculty of Basic Sciences - Laboratory of Vegetal Biotechnology - Cajicá/Zipaquirá, Cundinamarca - Colombia https://orcid.org/0000-0002-0200-5775
  • Carol Yineth Quinche Universidad Militar Nueva Granada - Faculty of Basic Sciences - Laboratory of Vegetal Biotechnology - Cajicá/Zipaquirá, Cundinamarca - Colombia https://orcid.org/0000-0002-0288-882X
  • Johana Carolina Soto Universidad Nacional de Colombia - Bogotá - Facultad de Ciencias - Departamento de Biología https://orcid.org/0000-0001-7890-4403
  • Ingrid Elizabeth Monroy Universidad Militar Nueva Granada - Faculty of Basic Sciences - Laboratory of Vegetal Biotechnology - Cajicá/Zipaquirá, Cundinamarca - Colombia https://orcid.org/0000-0001-8770-3232

The carnation is one of the most important products for export in the floriculture industry of Colombia. Fusariosis (a disease resulting from presence of Fusarium) appears on the crops in two forms: vascular wilt and basal rot. The first is caused by Fusarium oxysporum f. sp. dianthi, which is a well-characterized disease. The second, caused by Fusarium verticillioides (previously Fusarium roseum), is a non-characterized disease and its development in the plant has not been described in detail. The symptoms of basal rot were differentiated from vascular wilt, through infection of plants in the greenhouse, with isolates of F. verticillioides and F. oxysporum obtained from lesions of symptomatic plants. The fungi morphological characterization allowed differentiation of the isolates of these two species and their growth habits on different media. The sequencing of 8 different genes with more than 13 amplicons in the 2 species showed genetic differences that grouped the isolates into different taxa. Multilocus sequence typing analysis using DNA sequences of 8 different genetic regions confirmed the presence of F. verticillioides. In this study, the role of F. verticillioides was demonstrated in the stems of carnation in commercial crops that presented pathogenic lesions. According to the results of the study, F. verticillioides is the etiological agent that produces the basal rotting in carnation plants, alone or in association with F. oxysporum.

El clavel es uno de los productos más importantes para exportar en la industria floricultora de Colombia. La fusariosis (una enfermedad resultante de la presencia de Fusarium) aparece en el cultivo en dos formas: la marchitez vascular y la pudrición basal. La primera es causada por F. oxysporum f. sp. dianthi y es una enfermedad bien caracterizada. La segunda, causada por Fusarium verticillioides (anteriormente Fusarium roseum), es una enfermedad no caracterizada y su desarrollo en la planta no ha sido descrito en detalle. Los síntomas de la pudrición basal fueron diferenciados de los producidos por la marchitez vascular, a través de la infección de plantas en el invernadero, con aislados de F. verticillioides y F. oxysporum obtenidos de lesiones de plantas sintomáticas. La caracterización morfológica de los hongos permitió diferenciar las dos especies de Fusarium y se observaron sus hábitos de crecimiento en diferentes medios. Las secuencias de 8 diferentes genes con más de 13 diferentes amplicones en las 2 especies mostró diferencias genéticas que agruparon los aislados dentro de taxones diferentes. Análisis usando tipificación de secuencias múltiples de DNA confirmaron la presencia de F. verticillioides. En este estudio se estableció el papel de F. verticillioides presente en los tallos de clavel en cultivos comerciales que presentan lesiones patogénicas. De acuerdo con los resultados del estudio, F. verticillioides es el agente etiológico responsable de la pudrición basal en plantas de clavel solo o en asocio con F. oxysporum.

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How to Cite

APA

Filgueira Duarte, J. J., Rincón-Sandoval, C. M. ., Quinche, C. Y. ., Soto , J. C. . & Monroy , I. E. . (2022). Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg. Agronomía Colombiana, 40(1), 29–40. https://doi.org/10.15446/agron.colomb.v40n1.99009

ACM

[1]
Filgueira Duarte, J.J., Rincón-Sandoval, C.M. , Quinche, C.Y. , Soto , J.C. and Monroy , I.E. 2022. Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg. Agronomía Colombiana. 40, 1 (Jan. 2022), 29–40. DOI:https://doi.org/10.15446/agron.colomb.v40n1.99009.

ACS

(1)
Filgueira Duarte, J. J.; Rincón-Sandoval, C. M. .; Quinche, C. Y. .; Soto , J. C. .; Monroy , I. E. . Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg. Agron. Colomb. 2022, 40, 29-40.

ABNT

FILGUEIRA DUARTE, J. J.; RINCÓN-SANDOVAL, C. M. .; QUINCHE, C. Y. .; SOTO , J. C. .; MONROY , I. E. . Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg. Agronomía Colombiana, [S. l.], v. 40, n. 1, p. 29–40, 2022. DOI: 10.15446/agron.colomb.v40n1.99009. Disponível em: https://revistas.unal.edu.co/index.php/agrocol/article/view/99009. Acesso em: 13 nov. 2025.

Chicago

Filgueira Duarte, Juan José, Cindy Melissa Rincón-Sandoval, Carol Yineth Quinche, Johana Carolina Soto, and Ingrid Elizabeth Monroy. 2022. “Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg”. Agronomía Colombiana 40 (1):29-40. https://doi.org/10.15446/agron.colomb.v40n1.99009.

Harvard

Filgueira Duarte, J. J., Rincón-Sandoval, C. M. ., Quinche, C. Y. ., Soto , J. C. . and Monroy , I. E. . (2022) “Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg”, Agronomía Colombiana, 40(1), pp. 29–40. doi: 10.15446/agron.colomb.v40n1.99009.

IEEE

[1]
J. J. Filgueira Duarte, C. M. . Rincón-Sandoval, C. Y. . Quinche, J. C. . Soto, and I. E. . Monroy, “Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg”, Agron. Colomb., vol. 40, no. 1, pp. 29–40, Jan. 2022.

MLA

Filgueira Duarte, J. J., C. M. . Rincón-Sandoval, C. Y. . Quinche, J. C. . Soto, and I. E. . Monroy. “Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg”. Agronomía Colombiana, vol. 40, no. 1, Jan. 2022, pp. 29-40, doi:10.15446/agron.colomb.v40n1.99009.

Turabian

Filgueira Duarte, Juan José, Cindy Melissa Rincón-Sandoval, Carol Yineth Quinche, Johana Carolina Soto, and Ingrid Elizabeth Monroy. “Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg”. Agronomía Colombiana 40, no. 1 (January 1, 2022): 29–40. Accessed November 13, 2025. https://revistas.unal.edu.co/index.php/agrocol/article/view/99009.

Vancouver

1.
Filgueira Duarte JJ, Rincón-Sandoval CM, Quinche CY, Soto JC, Monroy IE. Basal rot in carnation (Dianthus caryophyllus L.) is caused by Fusarium verticillioides (Sacc.) Nirenberg. Agron. Colomb. [Internet]. 2022 Jan. 1 [cited 2025 Nov. 13];40(1):29-40. Available from: https://revistas.unal.edu.co/index.php/agrocol/article/view/99009

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