Publicado

2022-12-01

In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association

Propagación in vitro de una cepa cubana de Hongos Micorrízicos Arbusculares y su factible asociación a bacterias

DOI:

https://doi.org/10.15446/rev.colomb.biote.v24n2.100841

Palabras clave:

Arbuscular mycorrhizae, in vitro culture, MSR medium (en)
micorriza arbuscular, cultivo in vitro, medio SRM (es)

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Autores/as

  • Sussy Saymara Perera García Instituto Nacional de Ciencias Agrícolas https://orcid.org/0000-0001-5827-4168
  • Kalyanne Fernández Suárez Instituto Nacional de Ciencias Agrícolas
  • Eduardo José Pérez Ortega Instituto Nacional de Ciencias Agrícolas
  • Yonaisy Mujica Pérez Instituto Nacional de Ciencias Agrícolas
  • Reneé Pérez-Pérez Instituto Nacional de Ciencias Agrícolas
  • Yakelin Rodríguez Yon Instituto Nacional de Ciencias Agrícolas
  • Geert Haesaert Ghent University

In vitro root cultivation techniques based on modified root systems are often used in studies on Arbuscular Mycorrhizal Fungi (AMF). It is a simplified but powerful tool to investigate AMF root colonization and development of the extraradical mycelium. The aim of this study was to establish and characterize the in vitro culture of a Cuban strain of Rhizophagus irregularis (INCAM 11) by using transformed chicory roots. For that, superficially disinfected propagules of R. irregularis were co-culture with the hairy transformed chicory roots on Modified Strullu and Romand (MSR) medium during five months. Spore germination was observed 3-5 days after surface disinfection. The first contact between AMF hyphae and roots occurred 1 - 3 days after germination and a significant production of extensive extraradical mycelium was observed. New spore formation started within 21 - 25 days. After 5 months, 2000 spores could be observed per plate which were able to germinate, colonize, establish and reproduce again spores when associated to young transformed roots of chicory. The most frequent associated microorganism to the in vitro culture of INCAM 11 was isolated and identified as Paenibacillus sp.

Las técnicas de cultivo de raíces in vitro basadas en sistemas de raíces modificadas se utilizan a menudo en los estudios sobre hongos micorrízicos arbusculares (HMA). Es una herramienta simplificada pero poderosa para investigar la colonización de las raíces de los HMA y el desarrollo del micelio extrarradical. El objetivo de este estudio fue establecer y caracterizar el cultivo in vitro de una cepa cubana de Rhizophagus irregularis (INCAM 11) utilizando raíces transformadas de achicoria. Para ello, propágulos de R. irregularis desinfectados superficialmente fueron co-cultivados con las raíces transformadas de achicoria en medio Strullu y Romand modificado (SRM) durante cinco meses. La germinación de las esporas se observó 3-5 días después de la desinfección superficial. El primer contacto entre las hifas y las raíces se produjo entre 1 y 3 días después de la germinación y se observó una producción significativa de micelio extrarradical. La formación de nuevas esporas comenzó entre 21 - 25 días. Después de 5 meses, se pudieron observar 2000 esporas por placa que fueron capaces de germinar, colonizar, establecer y reproducir nuevas esporas cuando se asociaron a raíces jóvenes transformadas de achicoria. El microorganismo asociado frecuentemente al cultivo in vitro de INCAM 11 fue aislado e identificado como Paenibacillus sp.

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Cómo citar

APA

Perera García, S. S., Fernández Suárez, K., Pérez Ortega, E. J., Mujica Pérez, Y., Pérez-Pérez, R., Rodríguez Yon, Y. & Haesaert, G. (2022). In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association. Revista Colombiana de Biotecnología, 24(2), 36–45. https://doi.org/10.15446/rev.colomb.biote.v24n2.100841

ACM

[1]
Perera García, S.S., Fernández Suárez, K., Pérez Ortega, E.J., Mujica Pérez, Y., Pérez-Pérez, R., Rodríguez Yon, Y. y Haesaert, G. 2022. In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association. Revista Colombiana de Biotecnología. 24, 2 (dic. 2022), 36–45. DOI:https://doi.org/10.15446/rev.colomb.biote.v24n2.100841.

ACS

(1)
Perera García, S. S.; Fernández Suárez, K.; Pérez Ortega, E. J.; Mujica Pérez, Y.; Pérez-Pérez, R.; Rodríguez Yon, Y.; Haesaert, G. In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association. Rev. colomb. biotecnol. 2022, 24, 36-45.

ABNT

PERERA GARCÍA, S. S.; FERNÁNDEZ SUÁREZ, K.; PÉREZ ORTEGA, E. J.; MUJICA PÉREZ, Y.; PÉREZ-PÉREZ, R.; RODRÍGUEZ YON, Y.; HAESAERT, G. In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association. Revista Colombiana de Biotecnología, [S. l.], v. 24, n. 2, p. 36–45, 2022. DOI: 10.15446/rev.colomb.biote.v24n2.100841. Disponível em: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/100841. Acesso em: 16 mar. 2026.

Chicago

Perera García, Sussy Saymara, Kalyanne Fernández Suárez, Eduardo José Pérez Ortega, Yonaisy Mujica Pérez, Reneé Pérez-Pérez, Yakelin Rodríguez Yon, y Geert Haesaert. 2022. «In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association». Revista Colombiana De Biotecnología 24 (2):36-45. https://doi.org/10.15446/rev.colomb.biote.v24n2.100841.

Harvard

Perera García, S. S., Fernández Suárez, K., Pérez Ortega, E. J., Mujica Pérez, Y., Pérez-Pérez, R., Rodríguez Yon, Y. y Haesaert, G. (2022) «In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association», Revista Colombiana de Biotecnología, 24(2), pp. 36–45. doi: 10.15446/rev.colomb.biote.v24n2.100841.

IEEE

[1]
S. S. Perera García, «In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association», Rev. colomb. biotecnol., vol. 24, n.º 2, pp. 36–45, dic. 2022.

MLA

Perera García, S. S., K. Fernández Suárez, E. J. Pérez Ortega, Y. Mujica Pérez, R. Pérez-Pérez, Y. Rodríguez Yon, y G. Haesaert. «In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association». Revista Colombiana de Biotecnología, vol. 24, n.º 2, diciembre de 2022, pp. 36-45, doi:10.15446/rev.colomb.biote.v24n2.100841.

Turabian

Perera García, Sussy Saymara, Kalyanne Fernández Suárez, Eduardo José Pérez Ortega, Yonaisy Mujica Pérez, Reneé Pérez-Pérez, Yakelin Rodríguez Yon, y Geert Haesaert. «In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association». Revista Colombiana de Biotecnología 24, no. 2 (diciembre 1, 2022): 36–45. Accedido marzo 16, 2026. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/100841.

Vancouver

1.
Perera García SS, Fernández Suárez K, Pérez Ortega EJ, Mujica Pérez Y, Pérez-Pérez R, Rodríguez Yon Y, Haesaert G. In Vitro propagation of a cuban Arbuscular Mycorrhizal Fungal strain and factible bacteria’s association. Rev. colomb. biotecnol. [Internet]. 1 de diciembre de 2022 [citado 16 de marzo de 2026];24(2):36-45. Disponible en: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/100841

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CrossRef citations3

1. Jubair Al Rashid, Md Abuhena, Jingjing Wang, Md. Faisal Azim, Zhiyong Huang. (2025). Patching-up of major influencing factors of Rhizophagus irregularis cultivation for industrial production of consistent spore density through cascade statistical optimization. Bioresource Technology Reports, 30, p.102160. https://doi.org/10.1016/j.biteb.2025.102160.

2. Hassna Radi, Meriyem Koufan, Ilham Belkoura, Tayeb Koussa, Mouaad Amine Mazri. (2025). In Vitro Mycorrhization for Plant Propagation and Enhanced Resilience to Environmental Stress: A Review. Plants, 14(14), p.2097. https://doi.org/10.3390/plants14142097.

3. Marysol Romero-Ceciliano, Antonio Andrade-Torres, Evelyn Artavia-Salazar, Laura Yesenia Solís-Ramos. (2025). Establishing Monoxenic Culture of Arbuscular Mycorrhizal Fungus Glomus sp. Through In Vitro Root Organ Culture and Swietenia macrophylla King In Vitro Cultures. Agriculture, 15(7), p.673. https://doi.org/10.3390/agriculture15070673.

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