Publicado

2025-06-01

Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia

Optimización de la aclimatación ex vitro de Cinchona spp.: Hacia el establecimiento de un cultivo industrial en Colombia

DOI:

https://doi.org/10.15446/rev.colomb.biote.v27n1.118377

Palabras clave:

Cinchona, Ex Vitro transfer, Acclimatization, Unrooted shoots (en)
Cinchona, Ex vitro transfer, Acclimatization, Unrooted shoots (es)

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Efforts to optimize ex vitro transfer techniques of un-rooted in vitro shoots of Cinchona spp. (Rubiaceae) a native tree of the andean forests, are crucial for promoting sustainable cultivation practices of this medicinal plant. Renowned for its historical significance and effectiveness in treating malaria and other ailments, the tree holds a prominent position in botanical research, food industry, and pharmaceutical applications due to its production of alkaloids such as quinine and quinidine. This study aimed to improve the acclimatization process of un-rooted in vitro shoots of Cinchona, facilitating their transplantation to field conditions for establishing a plantation in Colombia. The results revealed that compared to commercial available substrates (pH higher than 5.5), the utilization of peat moss substrate (pH lower than 4 and electric conductivity lower than 100 μS / cm-1) led to significantly higher survival rates (>87%) and improved growth outcomes. This underscores the efficacy of peat moss in facilitating the acclimatization process of ex vitro plants over a 12-week period, ensuring robust development and survival of the plants, which guarantees vigorous specimens for field plantation.

Los esfuerzos para optimizar las técnicas de transferencia ex vitro de brotes no enraizados in vitro de Cinchona spp. (Rubiaceae), un árbol nativo de los bosques andinos, son cruciales para promover prácticas de cultivo sostenible de esta planta medicinal. Reconocida por su importancia histórica y su eficacia en el tratamiento de la malaria y otras dolencias, la planta ocupa una posición destacada en la investigación botánica, la industria alimentaria y las aplicaciones farmacéuticas debido a su producción de alcaloides como la quinina y la quinidina. Este estudio tuvo como objetivo mejorar el proceso de aclimatación de los brotes de Cinchona propagados in vitro no enraizados, facilitando su trasplante a condiciones de campo y sentando las bases para establecer una plantación en Colombia. Los resultados revelaron que, en comparación con los sustratos comerciales disponibles (pH superior a 5.5), la utilización de sustrato de turba (pH inferior a 4 y conductividad eléctrica inferior a 100 μS / cm-1) condujo a tasas de supervivencia significativamente más altas (>87%) y mejores resultados de crecimiento. Esto destaca la eficacia de la turba en la facilitación del proceso de aclimatación de plantas ex vitro durante un período de 12 semanas, asegurando un desarrollo y supervivencia robustos de las plantas, lo que garantiza especímenes vigorosos para la plantación en campo.

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Cómo citar

APA

Saavedra Correa, J. D., Bustamante Rodriguez, S. L. & Theiler, R. (2025). Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia. Revista Colombiana de Biotecnología, 27(1), 72–80. https://doi.org/10.15446/rev.colomb.biote.v27n1.118377

ACM

[1]
Saavedra Correa, J.D., Bustamante Rodriguez, S.L. y Theiler, R. 2025. Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia. Revista Colombiana de Biotecnología. 27, 1 (jun. 2025), 72–80. DOI:https://doi.org/10.15446/rev.colomb.biote.v27n1.118377.

ACS

(1)
Saavedra Correa, J. D.; Bustamante Rodriguez, S. L.; Theiler, R. Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia. Rev. colomb. biotecnol. 2025, 27, 72-80.

ABNT

SAAVEDRA CORREA, J. D.; BUSTAMANTE RODRIGUEZ, S. L.; THEILER, R. Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia. Revista Colombiana de Biotecnología, [S. l.], v. 27, n. 1, p. 72–80, 2025. DOI: 10.15446/rev.colomb.biote.v27n1.118377. Disponível em: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/118377. Acesso em: 24 dic. 2025.

Chicago

Saavedra Correa, Juan David, Silvia Lizette Bustamante Rodriguez, y Robert Theiler. 2025. «Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia». Revista Colombiana De Biotecnología 27 (1):72-80. https://doi.org/10.15446/rev.colomb.biote.v27n1.118377.

Harvard

Saavedra Correa, J. D., Bustamante Rodriguez, S. L. y Theiler, R. (2025) «Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia», Revista Colombiana de Biotecnología, 27(1), pp. 72–80. doi: 10.15446/rev.colomb.biote.v27n1.118377.

IEEE

[1]
J. D. Saavedra Correa, S. L. Bustamante Rodriguez, y R. Theiler, «Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia», Rev. colomb. biotecnol., vol. 27, n.º 1, pp. 72–80, jun. 2025.

MLA

Saavedra Correa, J. D., S. L. Bustamante Rodriguez, y R. Theiler. «Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia». Revista Colombiana de Biotecnología, vol. 27, n.º 1, junio de 2025, pp. 72-80, doi:10.15446/rev.colomb.biote.v27n1.118377.

Turabian

Saavedra Correa, Juan David, Silvia Lizette Bustamante Rodriguez, y Robert Theiler. «Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia». Revista Colombiana de Biotecnología 27, no. 1 (junio 4, 2025): 72–80. Accedido diciembre 24, 2025. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/118377.

Vancouver

1.
Saavedra Correa JD, Bustamante Rodriguez SL, Theiler R. Optimizing ex vitro Acclimatization of Cinchona spp.: Towards the establishment of an industrial crop in Colombia. Rev. colomb. biotecnol. [Internet]. 4 de junio de 2025 [citado 24 de diciembre de 2025];27(1):72-80. Disponible en: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/118377

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