Correo Electrónico
DNINFOA - SIA
Bibliotecas
Convocatorias
Identidad U.N.
Publicado
Efecto de Dicamba y de ácido 2,4 diclorofenoxiacético sobre la embriogénesis somática en caña de azúcar
Effect of Dicamba and 2,4 dichlorophenoxiacetic acid on sugarcane somatic embryogenesis
DOI:
https://doi.org/10.15446/rev.colomb.biote.v17n2.54280Palabras clave:
Cultivo in vitro, auxinas, vitroplántulas, Sacharum spp., embriogénesis somática, auxins, vitroplantlets, somatic embryos (es)Título en ingles: Efecto de Dicamba y de ácido 2,4 diclorofenoxiacético sobre la embriogénesis somática en caña de azúcar
El cultivo in vitro de la caña de azúcar ha sido establecido en muchas variedades comerciales con el propósito de producir material libre de enfermedades microbianas, conservar germoplasma, detectar resistencia a enfermedades y plagas, etc. En este sentido, el objetivo de este trabajo fue analizar la efectividad de las auxinas sintéticas ácido 2,4-diclorofenoxiacético (2,4D) y ácido 3,6-dicloro-2-metoxibenzoico (Dicamba), en la inducción del proceso de embriogénesis somática y la regeneración de vitroplántulas de distintas variedades de caña de azúcar (C26670, RB855546, V99245, V756, V781, V0050, CC8592, CC8475). Para esto se cultivaron discos de hojas en fase de macollamiento, de 1 cm de diámetro y 2 mm de grosor, en medio Murashige-Skoog, 1962 (MS) suplementado con 50 ml.l-1 agua de coco, 30 g.l-1 sacarosa y dos tratamientos diferentes: 3 mg.l-1 2,4-D ó 6.63 mg.l-1 Dicamba, ambos en completa oscuridad a 25ºC, durante 1 mes. Los callos obtenidos se colocaron en medio de regeneración, conteniendo ½ sales MS, 200 ml.l-1 agua de coco y 60 g.L-1 sacarosa, incubándose bajo luz continua, 25ºC, por 2 meses. El mayor porcentaje de callo embriogénico se obtuvo en medios suplementados con Dicamba un promedio de 70,83 % de callo embriogénico por variedad ; mientras que en los medios con 2,4D se obtuvo 62,08 % de callo embriogénico por variedad. Se obtuvo un promedio de 89,00 % de plantas regeneradas a partir de los callos obtenidos en medios con Dicamba y 66,12 % de plantas a partir de callos obtenidos en medios con 2,4D. Con el uso de Dicamba se estableció un sistema eficiente de embriogénesis somática para estas variedades de caña de azúcar.
In order to conserve sugarcane germplasm, produce microbial disease-free material, detect resistance to diseases and pests, etc., in vitro propagation of sugarcane has been established in many commercial varieties. In this sense, the aim of this work was to analyze the efficiency of 2,4-dichlorophenoxyacetic acid (2,4D) and 3,6-dichloro -2- methoxybenzoic acid (Dicamba) to induce somatic embryogenesis and regeneration of plantlets from sugarcane varieties C26670, RB855546, V99245, V756, V781, V0050, CC8592, CC8475. For induction of embryogenic callus, leaf discs in tillering stage of 1 cm diameter and 2 mm thick, were inoculated on Murashige-Skoog, 1962 medium (MS), supplemented with 50 ml.l-1 coconut water, 30 g.l-1sucrose and two different treatments: 3 mg.l-1 2,4D or 6.63 mg.l-1 Dicamba, both of them in total darkness at 25 °C, during 1 month. For plant regeneration, embryogenic calli were transferred to ½ MS salts supplemented with coconut water 200 ml.l-1 and sucrose 60 g.l-1 and incubated under continuous light, 25 °C, for 2 months. The highest percent of embryogenic callus induction was obtained in media supplemented with Dicamba, an average of 70.83 % of embryogenic callus by variety, while in media with 2,4-D, 62.08 % of embryogenic callus was obtained by variety. An average of 89,00 % of plantlets was obtained from calli induced on media with Dicamba and an average of 66.12% of plantlets was obtained from calli induced on media supplemented with 2,4D. Using Dicamba it was possible to establish an efficient somatic embryogenesis protocol for these sugarcane varieties.
Key words: in vitro culture, auxins, vitroplantlets, Sacharum spp, somatic embryos.
Citas
Abdi, G., Hedayat, M. (2011). Induction of somatic embryogenesis from immature zygotic embryo and immature seed of royal Poinciana (Delonixregia). World Applied Science Journal, 13, 391-395.
Brisibe, E., Miyake, H., Tanaguchi, T., Maeda, E. (1994). Regulation of somatic embryogenesis in long-terms callus cultures of sugarcane (Saccharum officinarum L.) New Phytology, 126, 301-307.
Cova, J., Nass, H., Orozco, A. (2006). Enfermedades de la caña de azúcar (Saccharum spp.) presentes en el estado Portuguesa durante el período 2003-2005. CENIAP HOY. N° 10. Disponible on line en: www.ceniap.inia.gov.ve (20/04/2007).
Falco, M., Janussi, B., Neto, T. (1996). Cell suspension culture of sugarcane: Growth, management and plant regeneration. Revista Brasilera de Fisiología Vegetal, 8,1-6.
FAO. (2014). Estadísticas de los cultivos más importantes del mundo. Disponible on line en: http://www.fao.org (10/03/2014).
Filippov, M., Miroshnichenko, D., Vernikovskaya, D., Dolgov, S. (2006). The effect of auxins,time exposure to auxin and genotypes on somatic embryogenesis from mature embryos of wheat. Plant Cell, Tissue and Organ Culture, 84, 213–222.
Gallo, M., English, R., Abouzid, A. (2000). Thidiazuron stimulates shoot regeneration of sugarcane embryogenic callus. In Vitro Cellular and Developmental Biology Plant, 36, 37-40.
Guiderdoni, E., Demarly, Y. (1988). Histology of somatic embryogenesis in cultured leaf segments of sugarcane plantlets. Plant Cell, Tissue and Organ Culture, 14,71-88.
Guiderdoni, E., Merot, B., Eksomtramage, T., Paulet, F., Feldmann, T., Glaszmann, J. E. (1995). Somatic embryogenesis in sugarcane (Saccharum species). En: Biotechnology in Agriculture and Forestry, Vol. 31. Somatic Embryogenesis and Synthetic Seed II (Ed. by Y.P.S. Bajaj). ©Springer-Verlag Berlin Heidelberg. 92-113.
Gnanapragasam, S., Vasil, I. (1990). Plant regeneration from a cryopreserved embryogenic cell suspension of a commercial sugarcane hybrid (Saccharum sp.). Plant Cell Report, 9,419-423.
Ho, W., Vasil, I. (1983). Somatic embryogenesis in sugarcane (Saccharum officinarum L.): the morphology and physiology of callus formation and the ontogeny of somatic embryos. Protoplasma, 118,169-184.
Joshi, S., Jain, M., Tillman, B., Altpeter, F., Gallo, M. (2013). Comparative analysis of direct plant regeneration from immature leaf whorl and floral explants for three elite US sugarcane (Saccharum spp. hybrids) genotypes. In Vitro Cellular and Developmental Biology, 49, 674-681.
Kaur, R., Kapoor, M. (2015). Plant regeneration through somatic embryogenesis in sugarcane. Sugar Tech. Disponible on line en: http://www.researchgate.net (10/05/2015).
Kaur, A., Sandhu, J. S. (2015). High throughput in vitro micropropagation of sugarcane (Saccharum officinarum L.) from spindle leaf roll segments: Cost analysis for agri-business industry. Plant Cell, Tissue and Organ Culture, 120, 339–350.
Lakshmanan, P., Geijskes, J., Aitken, K., Grof, C., Bonnett, G., Smith, G. (2005). Invited Review: Sugarcane Biotechnology: The challenges and opportunities. In Vitro Cellular and Developmental Biology Plant, 41, 345-363.
Manchanda, P., Gosal, S. (2012). Effect of activated charcoal, carbono sources and gelling agents on direct somatic embryogenesis and regeneration in sugarcane via leaf roll segments. SugarTechnology, 14, 168-173.
Marcano, A., Molina, P., Oropeza, M., de García, E. (2002). Optimización del proceso de embriogénesis somática en variedades venezolanas de caña de azúcar. Acta Científica Venezolana, 53, 251-257.
Mittal, P., Gosal, S., Senger, A., Kumar, P. (2009). Impact of cefotaxime on somatic embryogenesis and shoot regeneration in sugarcane. Physiology Molecular and Biology Plants, 15, 257-265.
Murashigue, T., Skoog, F. (1962). A revised medium for rapid growth and biossays with tobacco tissue culture. Plant Physiology, 15, 173-197.
Oropeza, M., García, E. (1996). Somaclonal variants resistant to sugarcane mosaic virus and their agronomic characterization. In Vitro Cellular and Developmental Biology Plant, 32, 26-30.
Oropeza, M., Marcano, A., García, E. (2001). Proteins related with embryogenic potential in callus and cell suspensions of sugarcane (Saccharum sp.). In Vitro Cellular and Developmental Biology Plant, 37, 211-216.
Raza, S., Qamarunisa, S., Hussain, M., Jamil, I., Anjum, S., Azhar, A., Ahmed, J. (2012). Regeneration in sugarcane via somatic embryogenesis and genomic instability in regenerated plants. Journal Crop Science Biotechnology, 15, 131-136.
Silva, M., Bedendo, I., Casagrande, M. (2005). Caracterização molecular e patogêncica de isolados de Xanthomonas albilineans (Ashby) Dowson agente causal escaldadura da cana de açúcar. Summa Phytopathology, 33, 341-347.
Silveira, V., Martins, A., Ferreira, A., Ribeiro, M., Segal, E., Santa, C. (2013). Morphological and polyamine content changes in embryogenic and non-embryogenic callus of sugarcane. Plant Cell, Tissue and Organ Culture, 114, 351-364.
Snyman, S., Gwethlyn, M., Aimee, C., Marzena, B., Paula, M. (2011). Applications of in vitro culture systems for commercial sugarcane production and improvement. In Vitro Cellular and Developmental Biology Plant, 47, 234-249.
Suprasanna, P., Choudhary, R., Desai, N., Bapat, V. (2005). Regulation of somatic embryogenesis by plant growth regulators in sugarcane. Sugar Technology, 7, 123-128.
Suprasanna, P., Patade, V. Y., Desai, N. S., Devarumath, R. M., Kawolr, P. G., Pagariya, M. C. & Babu, K. H. (2011). Biotechnological developments in sugarcane improvement: an overview. Sugar Technology, 13(4), 322-335.
Taylor, PWJ, Ko, HL, Adkins, SW, Rathus, C, Birch, RG (1992). Establishment of embryogenic callus and high protoplast yielding suspension cultures of sugarcane (Saccharum spp. hybrids). Plant Cell, Tissue and Organ Culture, 28, 69-78.
Taiz, L., Zeiger, E. (2010). Plant Physiology. Fifth edition. Sinauer Associates, Inc. U.S.A. p 782.
Taparia, Y., Gallo, M., Altpeter, F. (2012). Comparison of direct and indirect embryogenesis protocols, biolistic gene transfer and selection parameters for efficient genetic transformation of sugarcane. Plant Cell, Tissue and Organ Culture, 111, 131-141.
Thomas, C., Jiménez, V. (2005). Mode of action of plant hormones and plant growth regulators during induction of somatic embryogenesis: Molecular aspects. Plant Cell Monographs, 2, 157-175.
Watt, M., Banasiak, M., Reddy, D., Albertse, E., Snyman, S. (2009). In vitro minimal growth storage of Saccharum spp. hybrid (genotype 88H0019) at two stages of direct somatic embryogenic regeneration. Plant Cell, Tissue and Organ Culture, 96,263-271.
Licencia
Derechos de autor 2015 Revista Colombiana de Biotecnología
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
Esta es una revista de acceso abierto distribuida bajo los términos de la Licencia Creative Commons Atribución 4.0 Internacional (CC BY). Se permite el uso, distribución o reproducción en otros medios, siempre que se citen el autor(es) original y la revista, de conformidad con la práctica académica aceptada. El uso, distribución o reproducción está permitido desde que cumpla con estos términos.
Todo artículo sometido a la Revista debe estar acompañado de la carta de originalidad. DESCARGAR AQUI (español) (inglés).
