Publicado

2019-01-01

Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk

Producción de un pigmento antibacteriano con Serratia marcescens usando diferentes tipos de caseína obtenida de leche

DOI:

https://doi.org/10.15446/rev.colomb.biote.v21n1.62435

Palabras clave:

Bacterial pigments, Serratia marcescens, prodigiosin, protein substrates (en)
Pigmentos bacterianos, Serratia marcescens, prodigiosina, sustratos proteicos (es)

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Autores/as

  • Valentina Palacio-Castañeda Institución Universitaria Colegio Mayor de Antioquia - Facultad de Ciencias de la Salud
  • Alejandra Pérez-Hoyos Institución Universitaria Colegio Mayor de Antioquia - Facultad de Ciencias de la Salud
  • Daniel Carrascal-Correa Institución Universitaria Colegio Mayor de Antioquia - Facultad de Ciencias de la Salud
  • Victor Manuel Osorio-Echeverri Institución Universitaria Colegio Mayor de Antioquia - Facultad de Ciencias de la Salud
Prodigiosin,  a  pigment  produced  by Serratia  marcescensinhibits  the  growth  of  different  microorganisms  and  the  proliferation  of some human cancer cell lines. Prodigiosin is usually produced by fermentations of substrates such as starch and proteins, andpig-ment yield depends on the concentration of the carbon source, stirring speed of cultures, temperature and time of incubation,nitro-gen  sources,  and  pH  of  medium.  In  the  present  study,  pigment  production  was  assessed  using  two  types  of  casein  as  substrate; pigment yield was greater when casein precipitated with vinegar was used as substrate than when high purity casein was. The maxi-mum prodigiosin production was achieved with 10 g/L casein at pH 8.0. Additionally, supplementation of culture media with glu-cose was found to considerably decrease prodigiosin production and growth inhibition of Staphylococcus aureus, which is directly related to pigment yield. Production in stirred-tank bioreactor at 0.75 vvm aeration was higher than that at 0.5 and 1.0 vvm. Sub-strate type, concentration and pH affected pigment production in Erlenmeyer flasks, whereas aeration rate influenced pigment pro-duction in a stirred-tank bioreactor.
La prodigiosina es un pigmento producido por Serratia marcescensque inhibe el crecimiento de diferentes microorganismos y la proliferación de algunas líneas celulares de cáncer humano. La prodigiosina generalmente se produce a través de fermentaciones con sustratos como almidón y proteínas y su producción depende de la concentración de la fuente de carbono, la velocidad de agitación en los cultivos, la temperatura y el tiempo de incubación, las fuentes de nitrógeno y el pH del medio. En este trabajose evaluó la formación de pigmento usando dos tipos de caseína como sustrato; la producción de pigmento fue mayor cuando se utilizó caseína precipitada con vinagre que cuando se utilizó caseína de alta pureza. La máxima concentración de prodigiosinaselogró con 10 g/L de caseína a un pH de 8.0. Se encontró además que suplementar los medios de cultivo con glucosa disminuye considerablemente la producción de prodigiosina y la inhibición de Staphylococcus aureus, la cual está relacionada directamente con la producción de pigmento. La producción en biorreactor de tanque agitado con una aireación de 0.75 vvm fue mayor que con 0.5 y 1.0 vvm. El tipo de sustrato, la concentración y el pH afectaron la producción de pigmento en Erlenmeyer mientras que la velocidad de aireación influyó sobre la producción de pigmento en biorreactor de tanque agitado.

Referencias

Alihosseini, F., Ju, K.-S., Lango, J., Hammock, B. D., & Sun, G. (2008). Antibacterial colorants: characterization of Prodiginines and their applications on textile materials. Biotechnology Progress, 24(3), 742–7.

Aruldass, C. A., Venil, C. K., Zakaria, Z. A., & Ahmad, W. A. (2014). Brown sugar as a low-cost medium for the production of prodigiosin by locally isolated Serratia marcescens UTM1. International Biodeterioration & Biodegradation, 95, 19–24.

Babitha, S. (2009). Microbial pigments. En P. Nigam & A. Pandey (Eds.), Biotechnology for Agro-Industrial Residues Utilisation (p. 470). Dordrecht: Springer Netherlands.

Bertani, G. (1951). Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli. Journal of Bacteriology, 62(3), 293–300.

Bharmal, M. H., Jahagirdar, N., & Aruna, K. (2012). Study on optimization of prodigiosin production by Serratia marcescens MSK1 isolated from air. International Journal of Advanced Biological Research, 2(4), 671–680.

Casullo de Araújo, H. W., Fukushima, K., & Campos Takaki, G. M. (2010). Prodigiosin production by Serratia marcescens UCP 1549 using renewable-resources as a low cost substrate. Molecules, 15(10), 6931–40.

Dalili, D., Fouladdel, S., Rastkari, N., Samadi, N., Ahmadkhaniha, R., Ardavan, A., & Azizi, E. (2012). Prodigiosin, the red pigment of Serratia marcescens, shows cytotoxic effects and apoptosis induction in HT-29 and T47D cancer cell lines. Natural Product Research, 26(22), 2078–83.

Fender, J. E., Bender, C. M., Stella, N. A., Lahr, R. M., Kalivoda, E. J., & Shanks, R. M. Q. (2012). Serratia marcescens quinoprotein glucose dehydrogenase activity mediates medium acidification and inhibition of prodigiosin production by glucose. Applied and Environmental Microbiology, 78(17), 6225–35.

Gargallo, D., Lorén, J. G., Guinea, J., & Viñas, M. (1987). Glucose-6-phosphate dehydrogenase alloenzymes and their relationship to pigmentation in Serratia marcescens . Applied and Environmental Microbiology, 53(8), 1983–6.

Genes, C., Baquero, E., Echeverri, F., Maya, J. D., & Triana, O. (2011). Mitochondrial dysfunction in Trypanosoma cruzi: the role of Serratia marcescens prodigiosin in the alternative treatment of Chagas disease. Parasites & Vectors, 4, 66.

Giri, A. V, Anandkumar, N., Muthukumaran, G., & Pennathur, G. (2004). A novel medium for the enhanced cell growth and production of prodigiosin from Serratia marcescens isolated from soil. BMC Microbiology, 4, 1–10.

Heinemann, B., Howard, A. J., & Palocz, H. J. (1970). Influence of dissolved oxygen levels on production of L-asparaginase and prodigiosin by Serratia marcescens . Applied Microbiology, 19(5), 800–4.

Imhoff, J. F. (2005). Enterobacteriales. In G. Garrity, D. J. Brenner, N. R. Krieg, & J. R. Staley (Eds.), Bergey’s Manual of Systematic Bacteriology. Volume 2: The Proteobacteria Part B The Gammaproteobacteria (2nd ed., pp. 587–850). Boston, MA: Springer US.

Jensen, R. G. (1995). Handbook of Milk Composition. (R. G. Jensen, Ed.). San Diego, USA: Academic Press.

Kalbe, C., Marten, P., & Berg, G. (1996). Strains of the genus Serratia as beneficial rhizobacteria of oilseed rape with antifungal properties. Microbiological Research, 151(4), 433–9.

Kamble, K. D., & Hiwarale, V. D. (2012). Prodigiosin production from Serratia marcescens strains obtained from farm soil. International Journal of Environmental Sciences, 3(1), 631–8.

Kavitha, R., Aiswariya, S., & Ratnavali, C. M. G. (2010). Anticancer activity of red pigment from Serratia marcescens in Human cervix carcinoma. International Journal of PharmTech Research, 2(1), 784–7.

Kim, C.-H., Kim, S.-W., & Hong, S.-I. (1999). An integrated fermentation–separation process for the production of red pigment by Serratia sp. KH-95. Process Biochemistry, 35(5), 485–90.

Lane, D. (1991). 16S/23S rRNA sequencing. En E. Stackebrandt & M. Goodfellow (Eds.), Nucleic Acid Techniques in Bacterial Systematics (pp. 115–175). New York: John Wiley and Sons.

Matsumoto, K., Maeda, H., Takata, K., Kamata, R., & Okamura, R. (1984). Purification and characterization of four proteases from a clinical isolate of Serratia marcescens kums 3958. Journal of Bacteriology, 157(1), 225–32.

Pakhale, S. V., & Bhagwat, S. S. (2016). Purification of serratiopeptidase from Serratia marcescens NRRL B 23112 using ultrasound assisted three phase partitioning. Ultrasonics Sonochemistry, 31, 532–8.

Rahul, S., Chandrashekhar, P., Hemant, B., Chandrakant, N., Laxmikant, S., & Satish, P. (2014). Nematicidal activity of microbial pigment from Serratia marcescens . Natural Product Research, 28(17), 1399–404.

Roberts, D. P., McKenna, L. F., Lakshman, D. K., Meyer, S. L. F., Kong, H., de Souza, J. T., … Chung, S. (2007). Suppression of damping-off of cucumber caused by Pythium ultimum with live cells and extracts of Serratia marcescens N4-5. Soil Biology and Biochemistry, 39(9), 2275–88.

Song, M.-J., Bae, J., Lee, D.-S., Kim, C.-H., Kim, J.-S., Kim, S.-W., & Hong, S.-I. (2006). Purification and characterization of prodigiosin produced by integrated bioreactor from Serratia sp. KH-95. Journal of Bioscience and Bioengineering, 101(2), 157–61.

Southward, C. R. (n.d.). Casein products. Retrieved from http://nzic.org.nz/ChemProcesses/dairy/3E.pdf

Su, W.-T., Tsou, T.-Y., & Liu, H.-L. (2011). Response surface optimization of microbial prodigiosin production from Serratia marcescens . Journal of the Taiwan Institute of Chemical Engineers, 42(2), 217–22.

Sumathi, C., Mohanapriya, D., Swarnalatha, S., Dinesh, M. G., & Sekaran, G. (2014). Production of prodigiosin using tannery fleshing and evaluating its pharmacological effects. The Scientific World Journal, 2014, 8.

Suryawanshi, R. K., Patil, C. D., Borase, H. P., Salunke, B. K., & Patil, S. V. (2014). Studies on production and biological potential of prodigiosin by Serratia marcescens . Applied Biochemistry and Biotechnology, 173(5), 1209–21.

Toro Álvarez, C. M., Patiño Naranjo, P. A., & Ríos Estepa, R. (2001). Formulación de un medio de cultivo para la producción de pigmento a partir de Serratia marcescens . Revista Facultad de Ingeniería - Universidad de Antioquia, (23), 77–81.

Venil, C. K., & Lakshmanaperumalsamy, P. (2009). An insightful overview on microbial pigment, prodigiosin. Electronic Journal of Biology, 5(3), 49–61.

Wei, Y.-H., & Chen, W.-C. (2005). Enhanced production of prodigiosin-like pigment from Serratia marcescens SMdeltaR by medium improvement and oil-supplementation strategies. Journal of Bioscience and Bioengineering, 99(6), 616–22.

Yen, C.-H., Lin, Y.-S., & Tu, C.-F. (2015). A novel method for separation of caseins from milk by phosphates precipitation. Preparative Biochemistry & Biotechnology, 45(1), 18–32.

Cómo citar

APA

Palacio-Castañeda, V., Pérez-Hoyos, A., Carrascal-Correa, D. & Osorio-Echeverri, V. M. (2019). Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk. Revista Colombiana de Biotecnología, 21(1), 82–90. https://doi.org/10.15446/rev.colomb.biote.v21n1.62435

ACM

[1]
Palacio-Castañeda, V., Pérez-Hoyos, A., Carrascal-Correa, D. y Osorio-Echeverri, V.M. 2019. Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk. Revista Colombiana de Biotecnología. 21, 1 (ene. 2019), 82–90. DOI:https://doi.org/10.15446/rev.colomb.biote.v21n1.62435.

ACS

(1)
Palacio-Castañeda, V.; Pérez-Hoyos, A.; Carrascal-Correa, D.; Osorio-Echeverri, V. M. Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk. Rev. colomb. biotecnol. 2019, 21, 82-90.

ABNT

PALACIO-CASTAÑEDA, V.; PÉREZ-HOYOS, A.; CARRASCAL-CORREA, D.; OSORIO-ECHEVERRI, V. M. Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk. Revista Colombiana de Biotecnología, [S. l.], v. 21, n. 1, p. 82–90, 2019. DOI: 10.15446/rev.colomb.biote.v21n1.62435. Disponível em: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/62435. Acesso em: 16 mar. 2026.

Chicago

Palacio-Castañeda, Valentina, Alejandra Pérez-Hoyos, Daniel Carrascal-Correa, y Victor Manuel Osorio-Echeverri. 2019. «Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk». Revista Colombiana De Biotecnología 21 (1):82-90. https://doi.org/10.15446/rev.colomb.biote.v21n1.62435.

Harvard

Palacio-Castañeda, V., Pérez-Hoyos, A., Carrascal-Correa, D. y Osorio-Echeverri, V. M. (2019) «Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk», Revista Colombiana de Biotecnología, 21(1), pp. 82–90. doi: 10.15446/rev.colomb.biote.v21n1.62435.

IEEE

[1]
V. Palacio-Castañeda, A. Pérez-Hoyos, D. Carrascal-Correa, y V. M. Osorio-Echeverri, «Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk», Rev. colomb. biotecnol., vol. 21, n.º 1, pp. 82–90, ene. 2019.

MLA

Palacio-Castañeda, V., A. Pérez-Hoyos, D. Carrascal-Correa, y V. M. Osorio-Echeverri. «Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk». Revista Colombiana de Biotecnología, vol. 21, n.º 1, enero de 2019, pp. 82-90, doi:10.15446/rev.colomb.biote.v21n1.62435.

Turabian

Palacio-Castañeda, Valentina, Alejandra Pérez-Hoyos, Daniel Carrascal-Correa, y Victor Manuel Osorio-Echeverri. «Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk». Revista Colombiana de Biotecnología 21, no. 1 (enero 1, 2019): 82–90. Accedido marzo 16, 2026. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/62435.

Vancouver

1.
Palacio-Castañeda V, Pérez-Hoyos A, Carrascal-Correa D, Osorio-Echeverri VM. Antibacterial pigment production by Serratia marcescens using different casein types obtained from milk. Rev. colomb. biotecnol. [Internet]. 1 de enero de 2019 [citado 16 de marzo de 2026];21(1):82-90. Disponible en: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/62435

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CrossRef Cited-by

CrossRef citations6

1. Farhan Mohd Said. (2025). Plant Specialized Metabolites. Reference Series in Phytochemistry. , p.1135. https://doi.org/10.1007/978-3-031-51158-5_40.

2. Nazli Pinar Arslan. (2021). Use of wool protein hydrolysate as nitrogen source in production of microbial pigments. Journal of Food Processing and Preservation, 45(7) https://doi.org/10.1111/jfpp.15660.

3. Amin Derakhshanfar, Banafsheh Rastegari, Hossein Sharifi, Hossein Khajeh-Zadeh, Javad Moayedi. (2022). The effectiveness of antimicrobial photodynamic therapy with prodigiosin against reference strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. Lasers in Medical Science, 37(9), p.3631. https://doi.org/10.1007/s10103-022-03644-7.

4. Anjali Anjali, Vandana Sharma, Deepika Singh, Saurabh Saran. (2026). Enhanced production and purification of prodigiosin from a sequentially mutated strain of Serratia Sp MCA-3 using chia seeds as a substrate. Environmental Sustainability, https://doi.org/10.1007/s42398-025-00409-0.

5. Estrella Cervantes-García. (2024). Working with Vulnerable Populations. , p.19. https://doi.org/10.1007/978-3-031-67710-6_2.

6. Farhan Mohd Said. (2023). Plant Specialized Metabolites. Reference Series in Phytochemistry. , p.1. https://doi.org/10.1007/978-3-031-30037-0_40-1.

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