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Extraction of metabolites from Calendula officinalis and evaluation of their colorant and antibacterial capacity
Extracción de metabolitos desde Calendula officinalis y evaluación de su capacidad colorante y antimibacterial
extração de metabólitos de Calendula officinalis e avaliação de sua capacidade corante e antibacteriana
DOI:
https://doi.org/10.15446/rev.colomb.biote.v22n1.79999Palabras clave:
metabolites, dyeing ability, antimicrobial activity, natural additive, common marigold (en)metabolitos, capacidad de teñido, actividad antimicrobiana, aditivo natural, caléndula (es)
metabolitos, capacidade de tingimento, actividade antimicrobiana, aditivo natural, calêndula comum (pt)
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In this work, different bioactive compounds were obtained from Calendula officinalis by using conventional soxhlet extraction, shaker-assisted extraction and ultrasound-assisted extraction. The effect of different extraction time and temperature on the quantity of bioactive compounds obtained was investigated. The extracts obtained were further analyzed by UV–visible spectrometric analysis. The effect of extraction technique on both the colorant power of carotenoids and antibacterial capacity of the flavonoids was evaluate. Colorant power was evaluated by UV–visible spectrometric and dyeing ability in dairy product. Antibacterial activity of extracts was developed against Escherichia coli and Salmonella typhimurium by diffusion technique in well. Outstanding extraction results were obtained by ultrasound extraction technique performed during 2 hours, at 30°C and with 96% ethanol, for bioactive compounds evaluated. The same treatment showed the best result in colorant power and dyeing ability. The extracts obtained by ultrasound had a slightly inhibitory effect of growth on E. coli in comparison with the other techniques, while none of the extracts obtained from the different techniques reported a significant inhibition on the growth of S. Typhi. Therefore, ultrasound-assisted extraction is considered as an alternative process for obtaining the bioactive compounds from C. officinalis with high concentration, colorant power and antibacterial activity.
En este trabajo, se obtuvieron diferentes compuestos bioactivos de Calendula officinalis mediante el uso de la extracción por convencional por soxhlet, la extracción asistida por agitador y la extracción asistida por ultrasonido. Se investigó el efecto de diferentes tiempos y temperaturas de extracción en la cantidad de compuestos bioactivos obtenidos. Los extractos obtenidos se analizaron adicionalmente mediante análisis espectrométrico UV-visible. Se evaluó el efecto de la técnica de extracción tanto en el poder colorante de los carotenoides como en la capacidad antibacteriana de los flavonoides. El poder del colorante se evaluó mediante espectrometría UV visible y capacidad de teñido en productos lácteos. La actividad antibacteriana de los extractos se desarrolló contra Escherichia coli y Salmonella typhimurium mediante la técnica de difusión en pozo. Se obtuvieron excelentes resultados de extracción mediante una técnica de extracción por ultrasonido realizada durante 2 horas, a 30 ° C y con etanol al 96%, para los compuestos bioactivos evaluados. El mismo tratamiento mostró el mejor resultado en poder de colorante y capacidad de teñido. Los extractos obtenidos por ultrasonido tuvieron un efecto ligeramente inhibitorio del crecimiento en E. coli en comparación con las otras técnicas, mientras que ninguno de los extractos obtenidos de las diferentes técnicas informó una inhibición significativa en el crecimiento de S. Typhi. Por lo tanto, la extracción asistida por ultrasonido se considera un proceso alternativo para obtener los compuestos bioactivos de C. officinalis con alta concentración, poder colorante y actividad antibacteriana
Neste trabalho, diferentes compostos bioativos foram obtidos de Calendula officinalis usando extração convencional de soxhlet, extração assistida por shaker e extração assistida por ultra-som. O efeito de diferentes tempos de extração e temperatura na quantidade de compostos bioativos obtidos foi investigado. Os extratos obtidos foram posteriormente analisados por análise espectrométrica UV-visível. O efeito da técnica de extração tanto no poder corante dos carotenóides quanto na capacidade antibacteriana dos flavonóides foi avaliado. A potência dos corantes foi avaliada por espectrometria UV-visível e capacidade de tingimento em produtos lácteos. A atividade antibacteriana de extratos foi desenvolvida contra Escherichia coli e Salmonella typhimurium por técnica de difusão em poço. Resultados excelentes da extração foram obtidos pela técnica de extração ultra-sônica realizada durante 2 horas, a 30 ° C e com 96% de etanol, para compostos bioativos avaliados. O mesmo tratamento mostrou o melhor resultado em poder de corante e capacidade de tingimento. Os extratos obtidos por ultra-som tiveram um efeito inibitório do crescimento em E. coli em comparação com as outras técnicas, enquanto nenhum dos extratos obtidos das diferentes técnicas relataram uma inibição significativa no crescimento de S. Typhi. Portanto, a extração assistida por ultrassom é considerada um processo alternativo para a obtenção dos compostos bioativos de C. officinalis com alta concentração, poder de corante e atividade antibacteriana.
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