Publicado

2022-12-01

Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin

Caracterización de un Consorcio Microbiano Metanogénico de una Mina de Carbón en la Cuenca de Bogotá

DOI:

https://doi.org/10.15446/rev.colomb.biote.v24n2.93031

Palabras clave:

Geomicrobiology, coal mine, methane gas, 16S rRNA gene analysis (en)
Geomicrobiología, minas de carbón, gas metano, Análisis del gen 16S rARN (es)

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Autores/as

  • Claudia Marcela Mayorga Díaz Universidad Industrial de Santander
  • Edgar Pedraza Leguizamo Universidad Industrial de Santander
  • Oriana Danuta Serna Daza Universidad Industrial de Santander
  • Jorge Hernández Torres Universidad Industrial de Santander
  • Elena E. Stashenko Universidad Industrial de Santander
  • Mario García González Universidad Industrial de Santander
  • María Mercedes Levy Purdue University
  • Morris Levy Purdue University
  • Jorge Luis Lorenzo Universidad Industrial de Santander https://orcid.org/0000-0001-8112-0554

The work studied the methanogenic microbial consortium in a coal mine from the Bogotá basin in Colombia. Ex situ coal-enrichment cultures were established for in vitro growth and de novo gas production. Biogenic gas produced by cultures was analyzed by gas chromatography using thermal conductivity and flame ionization detectors. Cultures were used to isolate microbial specimens and to generate 16S rRNA gene libraries employing bacterial and archaeal primer sets. The gas chromatographic analysis showed methane production at 37 oC, but not at 60 oC, where CO2 was the major component of the biogenic gas. 16S rRNA gene sequence analysis of microbial isolates and clone libraries established that the methanogenic microbial consortium was formed by bacteria species from Bacillus and Gracilibacter genera plus archaea from the Methanothermobacter genus. This methanogenic microbial consortium was potentially responsible for biogenic gas generation in La Ciscuda coal mine. The results suggested that these methanogens produced methane by hydrogenotrophic or CO2 reduction pathways.

En el trabajo se estudió un consorcio microbiano metanogénico de una mina de carbón de la cuenca de Bogotá en Colombia. Se establecieron cultivos de enriquecimiento de carbón ex situ para el crecimiento y la producción de gas de novo. El gas biogénico producido por los cultivos se analizó mediante cromatografía de gases con detectores de ionización de llama y conductividad térmica. Los cultivos se utilizaron para aislar estirpes microbianas y para generar bibliotecas del gene 16S rRNA empleando de cebadores de bacteria y de arquea. El análisis de cromatografía de gases mostró producción de metano a 37 oC, pero no a 60 oC, donde el CO2 fue el componente principal del gas biogénico. El análisis de la secuencia del gen 16S rARN de estirpes microbianos y de las bibliotecas de clones, estableció que el consorcio microbiano metanogénico estuvo formado por especies de bacterias de los géneros Bacillus y Gracilibacter más la arquea del género Methanothermobacter. El consorcio microbiano metanogénico identificado es potencialmente responsable de la generación de gas biogénico en la mina de carbón La Ciscuda. Los resultados sugirieron que los metanógenos de este consorcio producían metano por vía hidrogenotrófica o de reducción de CO2.

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Cómo citar

APA

Mayorga Díaz, C. M., Pedraza Leguizamo, E., Serna Daza, O. D., Hernández Torres, J., Stashenko, E. E., García González, M., Levy, M. M., Levy, M. & Lorenzo, J. L. (2022). Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin. Revista Colombiana de Biotecnología, 24(2), 16–25. https://doi.org/10.15446/rev.colomb.biote.v24n2.93031

ACM

[1]
Mayorga Díaz, C.M., Pedraza Leguizamo, E., Serna Daza, O.D., Hernández Torres, J., Stashenko, E.E., García González, M., Levy, M.M., Levy, M. y Lorenzo, J.L. 2022. Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin. Revista Colombiana de Biotecnología. 24, 2 (dic. 2022), 16–25. DOI:https://doi.org/10.15446/rev.colomb.biote.v24n2.93031.

ACS

(1)
Mayorga Díaz, C. M.; Pedraza Leguizamo, E.; Serna Daza, O. D.; Hernández Torres, J.; Stashenko, E. E.; García González, M.; Levy, M. M.; Levy, M.; Lorenzo, J. L. Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin. Rev. colomb. biotecnol. 2022, 24, 16-25.

ABNT

MAYORGA DÍAZ, C. M.; PEDRAZA LEGUIZAMO, E.; SERNA DAZA, O. D.; HERNÁNDEZ TORRES, J.; STASHENKO, E. E.; GARCÍA GONZÁLEZ, M.; LEVY, M. M.; LEVY, M.; LORENZO, J. L. Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin. Revista Colombiana de Biotecnología, [S. l.], v. 24, n. 2, p. 16–25, 2022. DOI: 10.15446/rev.colomb.biote.v24n2.93031. Disponível em: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/93031. Acesso em: 16 mar. 2026.

Chicago

Mayorga Díaz, Claudia Marcela, Edgar Pedraza Leguizamo, Oriana Danuta Serna Daza, Jorge Hernández Torres, Elena E. Stashenko, Mario García González, María Mercedes Levy, Morris Levy, y Jorge Luis Lorenzo. 2022. «Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin». Revista Colombiana De Biotecnología 24 (2):16-25. https://doi.org/10.15446/rev.colomb.biote.v24n2.93031.

Harvard

Mayorga Díaz, C. M., Pedraza Leguizamo, E., Serna Daza, O. D., Hernández Torres, J., Stashenko, E. E., García González, M., Levy, M. M., Levy, M. y Lorenzo, J. L. (2022) «Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin», Revista Colombiana de Biotecnología, 24(2), pp. 16–25. doi: 10.15446/rev.colomb.biote.v24n2.93031.

IEEE

[1]
C. M. Mayorga Díaz, «Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin», Rev. colomb. biotecnol., vol. 24, n.º 2, pp. 16–25, dic. 2022.

MLA

Mayorga Díaz, C. M., E. Pedraza Leguizamo, O. D. Serna Daza, J. Hernández Torres, E. E. Stashenko, M. García González, M. M. Levy, M. Levy, y J. L. Lorenzo. «Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin». Revista Colombiana de Biotecnología, vol. 24, n.º 2, diciembre de 2022, pp. 16-25, doi:10.15446/rev.colomb.biote.v24n2.93031.

Turabian

Mayorga Díaz, Claudia Marcela, Edgar Pedraza Leguizamo, Oriana Danuta Serna Daza, Jorge Hernández Torres, Elena E. Stashenko, Mario García González, María Mercedes Levy, Morris Levy, y Jorge Luis Lorenzo. «Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin». Revista Colombiana de Biotecnología 24, no. 2 (diciembre 1, 2022): 16–25. Accedido marzo 16, 2026. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/93031.

Vancouver

1.
Mayorga Díaz CM, Pedraza Leguizamo E, Serna Daza OD, Hernández Torres J, Stashenko EE, García González M, Levy MM, Levy M, Lorenzo JL. Characterization of a Methanogenic Microbial Consortium from a Coal Mine in Bogotá Basin. Rev. colomb. biotecnol. [Internet]. 1 de diciembre de 2022 [citado 16 de marzo de 2026];24(2):16-25. Disponible en: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/93031

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1. Diptangshu Mukherjee, Vetrivel Angu Selvi, Jhuma Ganguly, Reginald Ebhin Masto. (2024). New insights into the coal-associated methane architect: the ancient archaebacteria. Archives of Microbiology, 206(5) https://doi.org/10.1007/s00203-024-03961-1.

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