Publicado

2022-01-31

Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana

In silico interaction of Agathisflavone, Amentoflavone and Punicalin molecules with human Importin α1

DOI:

https://doi.org/10.15446/rev.colomb.biote.v23n2.94466

Palabras clave:

Biflavonoides, Taninos, Carioferinas, Virus, Proteínas virales (es)
Biflavonoids, Tannin, Karyopherins, Viruses, Viral proteins (en)

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Autores/as

Varios virus con genoma de ARN en fases iniciales de la infección realizan la translocación de proteínas al interior del núcleo de la célula hospedera mediante la vía de las importinas α1. Este transporte es fundamental para el éxito de la replicación viral y se ha convertido en un blanco para la búsqueda y desarrollo de nuevos antivirales. El objetivo de este estudio fue determinar y caracterizar interacciones entre la Agatisflavona, Amentoflavona, Punicalina con el sitio mayor de unión de las Importinas α1 humanas mediante el análisis in silico del acoplamiento molecular y simulaciones de dinámica molecular. Las pruebas de acoplamiento molecular se realizaron entre estos fitoconstituyentes y la estructura de la importina α1 humana. Las afinidades de interacción fueron detectadas con la Agatisflavona, Amentoflavona y Punicalina (ΔGb = -8,8, -9,1 y -8,8 kcal.mol-1 respectivamente), con afinidades de interacción específicamente a los dominios ARM2–ARM5 (sitio mayor de unión) de las importinas α1. Las simulaciones de dinámica molecular revelaron interacciones significativamente favorables (P<0,001) con los ligandos Agatisflavona y Amentoflavona (ΔGb= -18,60±0,35 y -22,55±2,41 kcal.mol-1) mientras que la Punicalina registró mayores valores de energía de interacción (ΔGb= -5,33±1,72 kcal.mol-1). Los hallazgos obtenidos en este estudio computacional sugieren que las moléculas Agatisflavona y Amentoflavona presentan interacciones favorables con el sitio mayor de unión de las Importinas α1, en comparación a lo registrado con la Punicalina, sin embargo, se recomienda realizar ensayos in vitro a modo de confirmar estas observaciones.

Several RNA-viruses during early stages of infection perform the translocation of proteins into the nucleus of host cell by the importin α1 pathway. This transport is essential for viral replication success and has become a target to search and development new antivirals. The objective of this study was to determine and characterize interactions between Agathisflavone, Amentoflavone and Punicalin with the major binding site of human importins α1 by in silico analysis of molecular docking and molecular dynamics simulations. Molecular docking tests were performed between these phytoconstituents and the structure of human importin α1. Interaction’s affinity was detected with the Agathisflavone, Amentoflavone and Punicalin (ΔGb = -8.8, -9.1 and -8.8 kcal.mol-1 respectively), with binding affinity to ARM 2–ARM 5 domains (major binding site) of importins α1. Molecular dynamics simulations revealed significantly favorable interactions (P<0.001) with the ligands Agatisflavone and Amentoflavone (ΔGb = -18.60 ± 0.35 and -22.55 ± 2.41 kcal.mol-1) meanwhile Punicalin showed higher values of interaction free energy (ΔGb = -5.33 ± 1.72 kcal.mol-1). The findings obtained suggest that Agathisflavone and Amentoflavone could favorably interact to the major binding site of Importins α1 compared to that registered with Punicalin, however, it is recommended to perform in vitro assays to confirm these observations.

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APA

Gayozo, E. & Rojas, L. (2022). Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana. Revista Colombiana de Biotecnología, 23(2), 15–24. https://doi.org/10.15446/rev.colomb.biote.v23n2.94466

ACM

[1]
Gayozo, E. y Rojas, L. 2022. Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana. Revista Colombiana de Biotecnología. 23, 2 (ene. 2022), 15–24. DOI:https://doi.org/10.15446/rev.colomb.biote.v23n2.94466.

ACS

(1)
Gayozo, E.; Rojas, L. Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana. Rev. colomb. biotecnol. 2022, 23, 15-24.

ABNT

GAYOZO, E.; ROJAS, L. Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana. Revista Colombiana de Biotecnología, [S. l.], v. 23, n. 2, p. 15–24, 2022. DOI: 10.15446/rev.colomb.biote.v23n2.94466. Disponível em: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/94466. Acesso em: 28 dic. 2025.

Chicago

Gayozo, Elvio, y Laura Rojas. 2022. «Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana». Revista Colombiana De Biotecnología 23 (2):15-24. https://doi.org/10.15446/rev.colomb.biote.v23n2.94466.

Harvard

Gayozo, E. y Rojas, L. (2022) «Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana», Revista Colombiana de Biotecnología, 23(2), pp. 15–24. doi: 10.15446/rev.colomb.biote.v23n2.94466.

IEEE

[1]
E. Gayozo y L. Rojas, «Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana», Rev. colomb. biotecnol., vol. 23, n.º 2, pp. 15–24, ene. 2022.

MLA

Gayozo, E., y L. Rojas. «Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana». Revista Colombiana de Biotecnología, vol. 23, n.º 2, enero de 2022, pp. 15-24, doi:10.15446/rev.colomb.biote.v23n2.94466.

Turabian

Gayozo, Elvio, y Laura Rojas. «Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana». Revista Colombiana de Biotecnología 23, no. 2 (enero 31, 2022): 15–24. Accedido diciembre 28, 2025. https://revistas.unal.edu.co/index.php/biotecnologia/article/view/94466.

Vancouver

1.
Gayozo E, Rojas L. Interacción in silico de las moléculas Agathisflavona, Amentoflavona y Punicalina con la Importina α1 humana. Rev. colomb. biotecnol. [Internet]. 31 de enero de 2022 [citado 28 de diciembre de 2025];23(2):15-24. Disponible en: https://revistas.unal.edu.co/index.php/biotecnologia/article/view/94466

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1. Elvio Gayozo, Laura Rojas, Julio Barrios. (2025). Binding affinities analysis of ivermectin, nucleocapsid and ORF6 proteins of SARS-CoV-2 to human importins α isoforms: A computational approach. Biotecnia, 27, p.e2485. https://doi.org/10.18633/biotecnia.v27.2485.

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