Estrategia para el diseño de un medio de cultivo para la fermentación con bacillus
Huringiensis strategy to culture media design for the fermentation of bacillus thuringiensis
Keywords:
Bacillus thuringiensis, biopesticidas, medio de cultivo, fermentación (es)Bacillus thuringiensis, biopesticides, growth media, fermentation (en)
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En este trabajo se estudio el medio de cultivo para la fermentación con Bacillus thuringiensis tendiente a la producción del ingrediente activo de un biopesticida que emplea cepas nativas de esta bacteria. Se realizaron fermentaciones en matraz de 1000 mL de volumen total con 100 mL de volumen de fermentación, utilizando 10 mL de inóculo bacteriano, una temperatura de 29ºC y una velocidad de agitación de 200 revoluciones por minuto (rpm). El estudio se realizó empleando un modelo de diseño experimental con glucosa como fuente de carbono evaluando la concentración de las fuentes de minerales en el medio de cultivo para la cepa HD1. También se evaluó la relación óptima entre el nitrógeno orgánico y el nitrógeno inorgánico en el medio de cultivo, para la producción de proteína asociada al cristal para la cepa nativa Instituto de Biotecnología de la Universidad Nacional (IBUN) 28.5.
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Copyright (c) 1998 Revista Colombiana de Biotecnología
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