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Análisis de la secuencia de nucleótidos del gen de la capside y la polimerasa entre diferentes aislamientos del virus motoso del enanismo de la frambuesa
Sequence analysis of the capsid and polimerase genes of different raspberry bushy dwarf virus (rbdv) samples
Keywords:
Virus motoso, frambuesa, cápside, polimerasa (es)Raspberry, dwarf virus, capsid, polymerase (en)
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Este proyecto tuvo como objetivo determinar el grado de variabilidad existente al interior de las secuencias correspondientes al gen de la cápside y de la polimerasa entre cinco aislamientos de RBDV. Para este propósito, el DNA complementario (cDNA), correspondiente al gen de la cápside y de la polimerasa, fueron obtenidos por transcriptasa reversa y reacción en cadena de la polimerasa (RT-PCR), a partir de RNA de plantas inoculadas con cada uno de los respectivos aislamientos. El cDNA correspondiente al gen de la cápside y de la polimerasa, obtenido de cada aislamiento de RBDV, mediante esta metodología se clonó en el plásmido pGEM-T para ser secuenciado posteriormente. Los resultados mostraron que tanto el gen de la cápside, como el de la polimerasa, variaron menos del 1% entre estos aislamientos. Por lo tanto, se puede esperar que una secuencia transgénica de RBDV (de la cápside o de la polimerasa) que proteja contra un aislamiento de RBDV, podría también proteger contra otras cepas de RBDV.
This work was aimed to find sequence variability between capsid and polymerase gene sequences of five RBDV samples. Capsid and polymerase cDNAs were obtained by reverse transcription and PCR (RT-PCR) of RNA extracted from plants inoculated with each of the respective isolated samples. The amplified products were cloned in pGEM-T and sequenced. The results showed that the capsid and polymerase sequences varied less than 1% among the isolated samples. These data suggested that capsid and polymerase transgenic sequences that protect against a particular RBDV sample might protect against the others.
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Copyright (c) 1998 Revista Colombiana de Biotecnología
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