Comparación de las características poblacionales de Lemna minuta (ARACEAE: LEMNOIDEAE) en tres medios de cultivo
Comparison of the populational characteristics of Lemna minuta (ARACEAE: LEMNOIDEAE) in three culture media
DOI:
https://doi.org/10.15446/rev.colomb.biote.v20n1.50511Keywords:
medios de cultivo, tasa de crecimiento, bioensayo, Lemna minuta, Lemnoideae (es)culture media, growth rate, propagation, duckweed, Lemna minuta (en)
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Las investigaciones en macrófitas acuáticas neotropicales son escasas, principalmente en Colombia comparadas con países como Brasil, aunque se consideran comunidades apropiadas en diversas aplicaciones por su gran capacidad reproductiva y alta sensibilidad a condiciones cambiantes del ambiente. Se propuso aclimatar y cultivar un clon de Lemna minuta, lenteja de agua flotante de amplia distribución en Colombia y América. Sus frondas hijas se mantuvieron dos meses en el medio de cultivo APHA y posteriormente se comparó su propagación en tres medios de cultivo: Hoagland’s E+, APHA y AAP20x. Se analizaron variables de crecimiento poblacional como tasa de crecimiento, mortalidad, tiempo de duplicación y tiempo de vida. Adicionalmente, se evaluó la eficiencia del método de limpieza de frondas propuesto por Acreman para obtener cultivos axénicos. Los resultados indicaron que el medio Hoagland’s E+ (sin compuestos orgánicos) es el más adecuado para el crecimiento de las frondas en condiciones de laboratorio, debido a su mayor tasa de producción de frondas (0,16 frondas·d-1) y tiempo de vida (13,8 días), con menor mortalidad (0,11 frondas·d-1) y tiempo de duplicación (4,61 días). Conocer los parámetros de crecimiento poblacional y las condiciones de cultivo de L. minuta permiten proponerla como una macrófita relevante y candidata para diversos bioensayos de calidad de agua.
Although neotropical macrophytes are considered appropriate for diverse applications due to their great reproductive capacity and high sensitivity to changing environmental conditions, research on these plants is currently scarce, especially in Colombia when compared to countries such as Brazil. The current research work intended to acclimatize and cultivate a clone of the duckweed Lemna minuta, which is widely distributed in Colombia and America. After keeping daughter fronds of this species for two months in APHA culture medium, their propagation was compared in three culture media: Hoagland's E+, APHA and AAP20x. Population growth variables such as growth rate, mortality, doubling time and life span. Additionally, the efficiency of the frond cleaning method proposed by Acreman to obtain axenic cultures was evaluated. The results indicated that Hoagland's E+ medium (without organic compounds) is the most suitable one when it comes to frond growing under laboratory conditions, due to its associated higher frond production rate (0.16 fronds•d-1) and life span (13.8 d), as well as lower mortality (0.11 fronds•d-1) and doubling time (4.61 d). Knowing the population growth and cultivation conditions of L. minuta allows proposing it as a relevant macrophyte and candidate for various water quality bioassays.
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2. Daniel Ferley Ramírez-Babativa. (2019). Adaptación del método Acreman para la limpieza de plantas en Lemna minuta (Araceae: Lemnoideae). Revista Colombiana de Biotecnología, 21(1), p.128. https://doi.org/10.15446/rev.colomb.biote.v21n1.53219.
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