Correo Electrónico
DNINFOA - SIA
Bibliotecas
Convocatorias
Identidad U.N.
Published
Influencia de la luz azul sobre la productividad del cultivo sólido de Ganoderma lucidum
Influence of blue light on the productivity of the solid culture of Ganoderma lucidum
DOI:
https://doi.org/10.15446/rev.colomb.biote.v20n1.73674Keywords:
Basidiomycetes, fotoperiodo, desarrollo biológico, agroindustria, fermentación (es)Basidiomycetes, photoperiod, biological development, agroindustry, fermentation (en)
Downloads
Ganoderma lucidum es un hongo macromiceto reconocido por sus propiedades medicinales y su contenido de compuestos bioactivos que incluyen polisacáridos, triterpenoides, proteínas inmunomoduladoras, entre otros, lo que ha generado un incremento notable en su producción. La mayoría de especies de hongos responden y se adaptan a diversas señales ambientales incluida la luz, que favorece su productividad, tanto en calidad como en cantidad al estar estrechamente relacionada con la formación de cuerpos fructíferos. Por tal razón, el objetivo de este estudio fue evaluar la eficiencia biológica (EB) y la tasa de producción (TP) como parámetros de productividad del cultivo sólido de Ganoderma lucidum bajo irradiación de los sustratos con luz emitida por diodos azules (LED) con dos periodos de foto-estímulo de 12 y 24 h durante todas las fases de cultivo para inducir el crecimiento micelial y la formación de los cuerpos fructíferos. Se aplicaron parámetros convencionales para el crecimiento y desarrollo del hongo en las etapas de producción. Para la formulación de los sustratos, se emplearon residuos agroindustriales y materiales lignocelulósicos. El diámetro de los cuerpos fructíferos sometidos a tratamientos con luz azul fue mayor que los exhibidos a luz blanca fluorescente (Testigo). Los resultados muestran que el cultivo de Ganoderma lucidum con exposición a la luz azul es útil para la inducción de cuerpos fructíferos de alta calidad, logrando una disminución del periodo de fermentación en 16 días para el foto-estímulo de 24 h con EB de 28,04% y TP de 0,64.
Ganoderma lucidum, a renowned fungus for its medicinal properties and content of bioactive compounds include polysaccharides, triterpenoids, immune modulatory proteins, among others; it has generated a significant increase and interest in its production. Most species of fungi respond and adapt to various environmental signal including light, which affects it not only in productivity, but also in quality and quantity to be closely related to the fruiting bodies. Therefore, the objective of this study was evaluating the biological efficiency (EB) and the rate of production (TP) as production parameters of the solid culture of Ganoderma lucidum at under irradiation of the substrate with light emitting diodes blue (LED) with two levels of photo-stimulation constant 12 and 24 h during all steps of culture to induce mycelial growth and the formation of fruiting bodies. Conventional parameters were applied to stimulate the growth and development of the fungus in the stages of production. For the formulation of substrates agro-industrial waste and lignocellulosic materials were used. The diameter of the fruiting bodies under blue light treatments was higher than those exposed to white fluorescent light (control). The results show that the cultivation of Ganoderma lucidum with exposure to blue light is useful for induction of fruiting bodies of high quality, achieving a reduction of the period of fermentation in 16 days for the photo-stimulation of 24 h with EB of 28,04% and TP of 0,64.
References
Berovič, M., Habijanič, J., Zore, I., Wraber, B., Hodžar, D., Boh, B. & Pohleven, F. (2003). Submerged cultivation of Ganoderma lucidum biomass and immunostimulatory effects of fungal polysaccharides. Journal of Biotechnology, 103(1), 77-86.
Calonge, F. (2011). Hongos Medicinales. España: Mundi-Prensa. 130.
Corrochano, L. (2007). Fungal photoreceptors: Sensory molecules for fungal development and behaviour. Photochemical & Photobiological Sciences, 6(7), 725-736.
Chang, S. & Hayes, W. (2013). The Biology and Cultivation of Edible Mushrooms. Estados Unidos de América: Academic Press. 842.
Chang, S. & Miles, P. (2004). Mushrooms: Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact (2da ed.). Estados Unidos de América: CRC Press. 480.
Chiang, S., Liang, Z., Wang, Y. & Liang, C. (2017). Effect of light-emitting diodes on the production of cordycepin, mannitol and adenosine in solid-state fermented rice by Cordyceps militaris. Journal of Food Composition and Analysis, 60(Supplement C), 51-56.
Froehlich, A., Liu, Y., Loros, J. & Dunlap, J. (2002). White Collar-1, a Circadian Blue Light Photoreceptor, Binding to the frequency Promoter. Science, 297(5582), 815-819.
Fuller, K., Loros, J., & Dunlap, J. (2015). Fungal photobiology: visible light as a signal for stress, space and time. Current genetics, 61(3), 275-288.
González, M., Figlas, D., Devalis, R., Delmastro, S. & Curvetto, N. (2002). Sunflower seed hulls as a main nutrient source for cultivating Ganoderma lucidum. Micología Aplicada Internacional, 14(2), 19-24.
Henao, J., Montoya, S. & Segura, B. (2016). Estudio del efecto de la luz azul sobre varias actividades lignocelulolíticas y la producción de basidiomas del hongo Schizophyllum commune en un proceso de fermentación en estado sólido. Departamento de Ingeniería. Tesis de maestría. Universidad de Caldas. Manizales, 86.
Herrera‐Estrella, A. & Horwitz, B. (2007). Looking through the eyes of fungi: molecular genetics of photoreception. Molecular microbiology, 64(1), 5-15.
Huang, M., Lin, K., Lu, C., Chen, L., Hsiung, T. & Chang, W. (2017). The intensity of blue light-emitting diodes influences the antioxidant properties and sugar content of oyster mushrooms (Lentinus sajor-caju). Scientia Horticulturae, 218, 8-13.
Jang, M., Lee, Y., Ju, Y., Kim, S. & Koo, H. (2013). Effect of Color of Light Emitting Diode on Development of Fruit Body in Hypsizygus marmoreus. Mycobiology, 41(1), 63-66.
Ke, L., Wu, Q. & Zhang, D. (2011). Bioconversion of rape straw into a nutritionally enriched substrate by Ganoderma lucidum and yeast. African Journal of Biotechnology, 10(29), 5648-5653.
Kho, C., Kan, S., Chang, C., Cheng, H., Lin, C., Chiou, P. & Liu, Y. (2016). Analysis of exopolysaccharide production patterns of Cordyceps militaris under various light-emitting diodes. Biochemical Engineering Journal, 112, 226-232.
Kurtzman, R. & Martínez, C. (2013). Light, what it is and what it does for mycology. Micología Aplicada International, 25(2), 23-33.
Leatham, G. & Stahmann, M. (1987). Effect of light and aeration on fruiting of Lentinula edodes. Transactions of the British Mycological Society, 88(1), 9-20.
Lee, H., Song, M. & Hwang, S. (2003). Optimizing bioconversion of deproteinated cheese whey to mycelia of Ganoderma lucidum. Process Biochemistry, 38(12), 1685-1693.
Miyazaki, Y., Masuno, K., Abe, M., Nishizawa, H., Matsumoto, T., Kunitomo, S. & Kamada, T. (2011). Light-stimulative effects on the cultivation of edible mushrooms by using blue led. Villenave d'Ornon Cedex. Institut National de la Recherche Agronomique (INRA). 58-67.
Montoya, S., Sanchez, O. & Levin, L. (2013). Polysaccharide production by submerged and solid-state cultures from several medicinal higher Basidiomycetes. International Journal of Medicinal Mushrooms, 15(1), 71-79.
Paterson, R. (2006). Ganoderma – A therapeutic fungal biofactory. Phytochemistry, 67(18), 1985-2001.
Perkins, J. & Gordon, S. (1969). Morphogenesis in Schizophyllum commune. II. Effects of monochromatic light. Plant physiology, 44(12), 1712-1716.
Postemsky, P. & Curvetto, N. (2016). In Vitro Studies of Secondary Metabolite−Related Responses in Some Species of Genus Grifola (Agaricomycetes) from Argentina. 18(4), 355-363.
Rodríguez, V. & Jaramillo, C. (2005). Cultivo de hongos medicinales en residuos agrícolas de la zona cafetera. Boletín técnico Cenicafé, 28, 73.
Sakamoto, Y., Tamai, Y. & Yajima, T. (2004). Influence of light on the morphological changes that take place during the development of the Flammulina velutipes fruit body. Mycoscience, 45(5), 333-339.
Tisch, D. & Schmoll, M. (2010). Light regulation of metabolic pathways in fungi. Applied Microbiology and Biotechnology, 85(5), 1259-1277.
Vega, A. & Franco, H. (2013). Productividad y calidad de los cuerpos fructíferos de los hongos comestibles Pleurotus pulmonarius RN2 y P. djamor RN81 y RN82 cultivados sobre sustratos lignocelulósicos. Información tecnológica, 24, 69-78.
Wasser, S. (2013). Reishi or Ling Zhi (Ganoderma lucidum). In T. a. Francis (Ed.), Encyclopedia of Dietary Supplements. New York. (603-622).
Xie, C., Gong, W., Zhu, Z., Yan, L., Hu, Z. & Peng, Y. (2018). Comparative transcriptomics of Pleurotus eryngii reveals blue-light regulation of carbohydrate-active enzymes (CAZymes) expression at primordium differentiated into fruiting body stage. Genomics, en publicación, 110 (3), 201-209.
Xie, Ch., Yan, Y., Gong, W., Zhu, Z., Tan, S., Chen, D., Hu, Z. & Peng, Y. (2016a). Effects of Different Substrates on Lignocellulosic Enzyme Expression, Enzyme Activity, Substrate Utilization and Biologycal efficient of Pleurotus Eryngii. Celular Physiology and Biochemistry, 39 (4), 1479-1494.
Xie, C., Luo, W., Li, Z., Yan, L., Zhu, Z., Wang, J., Hu, Z. & Peng, Y. (2016b). Secretome analysis of Pleurotus eryngii reveals enzymatic composition for ramie stalk degradation. Electrophoresis, 37 (2), 310-320.
Zapata, P., Rojas, D., Ramirez, D., Fernandez, C. & Atehortua, L. (2009). Effect of different light-emitting diodes on mycelial biomass production of Ling Zhi or Reishi medicinal mushroom Ganoderma lucidum (W. Curt.: Fr.) P. Karst. (Aphyllophoromycetideae). International Journal of Medicinal Mushrooms, 11(1), 93-99.
Zhou, X., Su, K. y Zhang, Y. (2012). Applied modern biotechnology for cultivation of Ganoderma and development of their products. Applied Microbiology and Biotechnology, 93(3), 941-963.
How to Cite
APA
ACM
ACS
ABNT
Chicago
Harvard
IEEE
MLA
Turabian
Vancouver
Download Citation
CrossRef Cited-by
1. Sandra Montoya Barreto, Gloria María Restrepo Franco, Claudia Lorena Calvo Rodríguez. (2022). Actividades ligninolíticas y producción de basidiomas de Ganoderma lucidum en cultivo sólido suplementado con manganeso. Revista Colombiana de Biotecnología, 24(2), p.46. https://doi.org/10.15446/rev.colomb.biote.v24n2.95288.
Dimensions
PlumX
Article abstract page views
Downloads
License
Copyright (c) 2018 Revista Colombiana de Biotecnología
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish in the journal are welcome to code Creative Commons 4.0 Attribution, non-commercial, no derivatives. That is, that even as the Colombian Journal of Biotechnology of free access, users can download the information contained in it, but should give attribution or recognition of intellectual property, should use it as is, without derivation and should not be used with commercial purposes. Creative Commons This work is under a Creative Commons Attribution 4.0 License International. You are free: Share - copy and redistribute the material in any media or format Adapt - remix, transform and create from the material for any purpose, including commercial. The licensor can not revoke these freedoms while complying with the terms of the license. Under the following conditions: Attribution - You must properly acknowledge authorship, provide a link to the license and indicate whether changes have been made <. You can do this in any reasonable manner, but not in a way that suggests that support the licensor or receives for his use. No additional restrictions - You can not apply legal terms or technological measures that legally restrict realize what the license allows. notices: You do not have to comply with the license for those elements of the material in the public domain or where their use is permitted by the application of an exception or a limit. No guarantees are given. The license can not provide all the necessary permits for the intended use. For example, other rights such as advertising, privacy, or moral rights may limit the use of the material.
