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Production of PHA under improved culture conditions and using a new feeding model
Producción de PHA bajo condiciones de cultivo mejoradas y empleando un nuevo modelo de alimentación
Palabras clave:
biopolymers; polyhydroxyalkanoates; productivity; fed batch; mathematical model (en)biopolímeros; polihidroxialcanoatos; productividad; lote alimentado; modelo matemático (es)
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Polyhydroxyalkanoates (PHAs) have become important due to their mechanical properties, which resemble those of plastics derived from petrochemicals. Despite the advantages they possess, its use is limited by production costs. In terms of the culture systems used for process improvement, fed batch systems are the most studied, since they allow higher productivity to be obtained. In this study, PHAs production was reviewed with a new feeding model against the reported exponential model and under improved culture conditions. In the results, the predictive feeding strategy allowed to improve the production of PHAs (24.1 ± 1.14 g/L) by controlling the concentration of residual substrate, with respect to the exponential model (18.1 ± 1.33 g/L), achieving a biomass concentration of 40.9 ± 1.12 g/L and a polymer productivity of 1.01 gPHA/L.h, improving the process by 25%.
Los polihidroxialcanoatos (PHA) han tomado importancia debido a que poseen propiedades mecánicas semejantes a los plásticos de origen petroquímico. A pesar de las ventajas que poseen, su uso está limitado por los costos de producción. A nivel de los sistemas de cultivo empleados para el mejoramiento del proceso, los sistemas por lote alimentado son los más estudiados, ya que permiten obtener mayores productividades. En este estudio, se evalúo la producción de PHA con un nuevo modelo de alimentación frente al modelo exponencial reportado y bajo condiciones de cultivo mejoradas. En los resultados, la estrategia de alimentación predictiva permitió mejorar la producción de PHA (24.1 ± 1.14 g/L) al controlar la concentración de sustrato residual, respecto al modelo exponencial (18.1 ± 1.33 g/L), logrando una concentración de biomasa de 40.9 ± 1.12 g/L y una productividad de polímero de 1.01 gPHA/L.h, mejorando el proceso en un 25%.
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