Location of the sampling stations at the Macapule Lagoon (Station 1: northern mouth, 25.37° N, 108.74° W, Station 2: El Tortugón estuary, 25.39° N, 108.67° W) and Finca Doña Luisa shrimp farm (closed circle).

Publicado

2024-02-16

Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California

Detección del virus del síndrome de la mancha blanca en un ecosistema costero y una granja de camarón en el Golfo de California

DOI:

https://doi.org/10.15446/dyna.v91n231.110250

Palabras clave:

WSSV; seston size-fractions; nested PCR; real-time PCR; Macapule lagoon (en)
WSSV; fracciones por tamaño de seston; PCR anidada; PCR en tiempo real; Laguna de Macapule. (es)

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Three molecular assays were used to detect and quantify white spot syndrome virus (WSSV) in DNA extracted from seston size-fractioned (0.02, 0.2, 1.2, and 20 μm) samples collected from a coastal lagoon and an adjacent shrimp farm. From 107 DNA extracts, only two from one sample tested positive for WSSV with nested PCR in the 1.2 and 20 μm fractions. These results were confirmed by a semi-quantitative (IQ2000TM WSSV Detection and Prevention System) and a quantitative (IQREALTM WSSV Quantitative System) detection system based, based, respectively, on nested PCR and real-time PCR. A first viral load reference value (6.54 × 104 WSSV copies/mL) was established in a seston size fraction (1.2−20 μm). The results suggest that WSSV could be associated with both resuspension of fine clays and silts, and nanoplankton and organic colloids during infectious events.

Con el fin de detectar y cuantificar el virus del síndrome de las manchas blancas (WSSV) en extractos de ADN de muestras de seston fraccionadas por tamaño (0.02, 0.2, 1.2 y 20 μm) de una laguna costera y una granja camaronícola contigua se emplearon tres ensayos moleculares. De 107 extractos de ADN, solo dos de una muestra resultaron con detección positiva al WSSV con PCR anidada en las fracciones de 1.2 y 20 μm. Estos resultados fueron confirmados por un sistema de detección semicuantitativo (IQ2000TM WSSV) y uno cuantitativo (IQREALTM WSSV) basados, respectivamente, en PCR anidada y en PCR en tiempo real. Se estableció un primer valor de referencia de carga viral (6.54×104 copias WSSV/mL) en una fracción de tamaño de seston (1.2‒20μm). Los resultados sugieren que, durante eventos infecciosos, el WSSV podría estar asociado tanto a la resuspensión de arcillas y limos finos, como a nanoplancton y coloides orgánicos.

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Cómo citar

IEEE

[1]
C. Hakspiel-Segura, A. Martínez-López, M. López-Meyer, y D. C. Escobedo-Urías, «Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California», DYNA, vol. 91, n.º 231, pp. 63–68, ene. 2024.

ACM

[1]
Hakspiel-Segura, C., Martínez-López, A., López-Meyer, M. y Escobedo-Urías, D.C. 2024. Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California. DYNA. 91, 231 (ene. 2024), 63–68. DOI:https://doi.org/10.15446/dyna.v91n231.110250.

ACS

(1)
Hakspiel-Segura, C.; Martínez-López, A.; López-Meyer, M.; Escobedo-Urías, D. C. Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California. DYNA 2024, 91, 63-68.

APA

Hakspiel-Segura, C., Martínez-López, A., López-Meyer, M. y Escobedo-Urías, D. C. (2024). Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California. DYNA, 91(231), 63–68. https://doi.org/10.15446/dyna.v91n231.110250

ABNT

HAKSPIEL-SEGURA, C.; MARTÍNEZ-LÓPEZ, A.; LÓPEZ-MEYER, M.; ESCOBEDO-URÍAS, D. C. Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California. DYNA, [S. l.], v. 91, n. 231, p. 63–68, 2024. DOI: 10.15446/dyna.v91n231.110250. Disponível em: https://revistas.unal.edu.co/index.php/dyna/article/view/110250. Acesso em: 11 oct. 2024.

Chicago

Hakspiel-Segura, Cristian, Aída Martínez-López, Melina López-Meyer, y Diana Cecilia Escobedo-Urías. 2024. «Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California». DYNA 91 (231):63-68. https://doi.org/10.15446/dyna.v91n231.110250.

Harvard

Hakspiel-Segura, C., Martínez-López, A., López-Meyer, M. y Escobedo-Urías, D. C. (2024) «Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California», DYNA, 91(231), pp. 63–68. doi: 10.15446/dyna.v91n231.110250.

MLA

Hakspiel-Segura, C., A. Martínez-López, M. López-Meyer, y D. C. Escobedo-Urías. «Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California». DYNA, vol. 91, n.º 231, enero de 2024, pp. 63-68, doi:10.15446/dyna.v91n231.110250.

Turabian

Hakspiel-Segura, Cristian, Aída Martínez-López, Melina López-Meyer, y Diana Cecilia Escobedo-Urías. «Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California». DYNA 91, no. 231 (enero 24, 2024): 63–68. Accedido octubre 11, 2024. https://revistas.unal.edu.co/index.php/dyna/article/view/110250.

Vancouver

1.
Hakspiel-Segura C, Martínez-López A, López-Meyer M, Escobedo-Urías DC. Detection of white spot syndrome virus in seston from a coastal ecosystem and a shrimp farm in the Gulf of California. DYNA [Internet]. 24 de enero de 2024 [citado 11 de octubre de 2024];91(231):63-8. Disponible en: https://revistas.unal.edu.co/index.php/dyna/article/view/110250

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