Publicado

2017-10-01

Fast estimation of chlorophyll content on plant leaves using the light sensor of a smartphone

Estimación rápida del contenido de clorofila en hojas usando el sensor de luz de un teléfono celular

Palabras clave:

Plant nutrition, non-destructive chlorophyll meter, leaf light transmittance (en)
Nutrición vegetal, medición no destructiva de clorofila, transmisión de luz por hojas (es)

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Plant chlorophyll measurements can support nitrogen fertilization decisions. Using a 3D printed device and a red LED, here we tested the feasibility of using a smartphone ambient light sensor (ALS) to estimate leaf chlorophyll by light transmission. Its performance was evaluated by comparing 30 leaf sorghum (Sorghum bicolor (L.) Moench) transmission readings of the red LED (663 nm) obtained from the smartphone and a standard spectrometer, which showed a good coefficient of determination (r2 = 0.9067). Additionally, a comparison between the ALS and a SPAD 502TM (a commercial device for chlorophyll content estimation) was made with chrysanthemum (Dendranthema grandiflora Tzvelev) leaves, obtaining a good correlation between both measurements. Light transmission was also measured in S. bicolor flag leaves from plants growing in the greenhouse under increasing nitrogen fertilization. A clear fit between leaf light transmission and plant height was also observed, suggesting a simple, smartphone based estimation of plant’s chlorophyll.
Medir clorofila puede apoyar decisiones de fertilización nitrogenada de plantas. Usando un dispositivo impreso en 3D y un LED rojo, aquí probamos la factibilidad de usar un teléfono celular para estimar clorofila por transmisión de luz. Al comparar 30 lecturas de trasmisión de luz roja (663 nm) por hojas de sorgo Sorghum bicolor (L.) Moench, obtenidas del teléfono y de un espectrómetro estándar, se encontró un buen coeficiente de determinación (r2 = 0.9067). Adicionalmente, se hizo una comparación entre las lecturas del teléfono y un SPAD 502TM (un dispositivo comercial para la estimación del contenido de clorofila) en hojas de crisantemo Dendranthema grandiflora Tzvelev, obteniendo una buena correlación entre ambas medidas. La transmisión medida en hojas de S. bicolor de plantas de invernadero fertilizadas con nitrógeno también presentó buen ajuste con la altura de las plantas, sugiriendo la posibilidad de estimar fácilmente el contenido de clorofila en hojas usando teléfonos inteligentes.

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