Publicado

2018-01-01

Enzymatic modification of cassava starch (Corpoica M-Tai) around the pasting temperature

Modificación enzimática de almidón de yuca (Corpoica M-Tai) alrededor de la temperatura de pasta

DOI:

https://doi.org/10.15446/dyna.v85n204.66620

Palabras clave:

α-amylase, granule, crystallinity, hydrolyzed starches (en)
α-amilasa, granulo, cristalinidad, almidones hidrolizados (es)

Autores/as

Cassava starch variety Corpoica – TAI, was modified with a commercial α-amylase, in order to evaluate the effects of temperature, reaction time and enzyme/substrate (E/S) ratio on the degree of hydrolysis, and production of enzymatic modified starches. The modified starches presented dextrose equivalents (DE) ranging between 13 and 92%, yield (Y) between 0 and 45.5%. The Scanning electron microscope (SEM) analysis showed that the granules suffered exo-corrosion in agreement with the degree of hydrolysis. The crystallinity degree increased following modification. The granule morphology of hydrolyzed starch was different compared to the native starch. The gelatinization temperature in the residues of hydrolyzed cassava starches hydrolyzed increased with the crystallinity degree as well with the reduction in the amylose content. The native starch showed a greater setback and amylose values than hydrolyzed starches, which indicates that it is more susceptible to retrogradation or water lost (syneresis).
Almidón de yuca variedad Corpoica – TAI fue modificado con α-amilasa comercial, para evaluar el efecto de la temperatura alrededor de la temperatura de pasta, tiempo de reacción y relación enzima/sustrato (E/S) sobre el grado de hidrólisis y producción de almidones modificados enzimáticamente. Los almidones modificados presentaron equivalentes de dextrosa (DE) entre 13 y 92 %, valores de rendimiento (Y) hasta del 45.5%. El análisis Microscopia de barrido electrónico (SEM) mostró que los gránulos presentaron exo-corrosión en concordancia con el grado de hidrólisis. La cristalinidad aumento después de la modificación y la morfología del granulo fue diferente comparada con el almidón nativo. Los almidones hidrolizados presentaron un aumento en la estabilidad (breakdown) y disminución en el asentamiento (setback). La temperatura de gelatinización se incrementó con la cristalinidad y disminuyó con el contenido de amilosa. Los almidones hidrolizados fueron más susceptibles a la retrogradación o pérdida de agua (sinéresis).

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Hoover, R. and Zhou, Y., In vitro and in vivo hydrolysis of starches by α-amylase and resistant starch formation in legumes – A review. Carbohydrate Polymers, 54(4), pp. 401-417, 2003. DOI: 10.1016/S0144-8617(03)00180-2

Shariffa, Y.N., Karim, A.A., Fazilah, A. and Zaidul, I.S., Enzymatic hydrolysis of granular native and mildly heat-treated tapioca and sweet potato starches at sub-gelatinization temperature. Food Hydrocolloids, 23(2), pp. 434-440, 2009. DOI: 10.1016/j.foodhyd.2008.03.009

Li, J.H., Vasanthan, T., Hoover, R. and Rossnagel, B.G., Starch from hull-less barley: V. In-vitro susceptibility of waxy, normal, and high-amylose starches towards hydrolysis by alpha-amylases and amyloglucosidase. Food Chemistry, 84(4), pp. 621-632, 2004. DOI: 10.1016/S0308-8146(03)00287-5

Tester, R.F., Qi, X. and Karkalas, J., Hydrolysis of native starches with amylases. Animal Feed Science and Technology 130(1-2), pp. 39-54, 2006. DOI: 10.1016/j.anifeedsci.2006.01.016

Rocha, T.S., Carneiro, A.P. and Franco, C.M., Effect of enzymatic hydrolysis on some physicochemical properties of root and tuber granular starches. Ciência e Tecnologia de Alimentos, 30(2), pp. 544-551, 2010. DOI: 10.1590/S0101-20612010000200039

Franco, C.M., Preto, R.S. and Ciacco, C.F., Factors that affect the enzymatic degradation of natural starch granules: Effect of size of the granules. Starch/Stärke, 44(11), pp. 422-426, 1992. DOI: 10.1002/star.19920441106

Noda, T., Takigawa, S., Matsuura, E.C., Saito, K., Takata, K., Tabiki, T., Mangalika, W.H. and Yamauchi, H., The physicochemical properties of partially digested starch from sprouted wheat grain. Carbohydrate Polymers, 56(3), pp. 271-277, 2004. DOI: 10.1016/j.carbpol.2003.10.015

Matute, L., Bertsch, A. y Diaz, I., Evaluación de la actividad amilolítica de Aspergillus niger (ANM-1) en fermentaciones en estado sólido y sumergido para la obtención y caracterización de aditivos enzimáticos. Revista Facultad de Agronomia (UCV), 38(1), pp. 9-17, 2012.

Miller, G.L., Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(1), pp. 426-428, 1959. DOI: 10.1021/ac60147a030

Sánchez, L.H., Solorza, J.F., Méndez, M.G., Paredes, L.O. and Bello, L.P., Isolation and partial characterization of Okenia (Okenia hypogaea) starch. Starch/Stärke, 54(5), pp. 193-197, 2002. DOI: 10.1002/star.200300235

Nara, S. and Komiya, T.T., Studied on the relationship between water-saturated state and crystallinity by the diffraction method for moistened potato starch. Starch/Starke, 35, pp. 407-410, 1983. DOI: 10.1002/star.19830351202

Frost, K., Kaminski, D., Kirwan, G., Lascaris, E. and Shanks, R., Crystallinity and structure of starch using wide angle X-ray scattering. Carbohydrate Polymers, 78(3), pp. 543-548, 2009. DOI: 10.1016/j.carbpol.2009.05.018

Colussi, R., Mello, S., Zanella, V., Bartz, J., Gutkoski, L., Zavareze, E. and Guerra, A., Acetylation of rice starch in an aqueous medium for use in food. LWT - Food Science and Technology, 62(2), pp. 1076-1082, 2015. DOI: 10.1016/j.lwt.2015.01.053

International Standardization Organization (ISO). Rice- Determination of amylose content. Part 2: Routine Method, ISO 6647-2. Switzerland: ISO, 2003.

Schoch, T.J., Swelling power and solubility of granular starches, in Whistler, R.L. Methods in carbohydrate chemistry. London: Academic Press, 1964. pp. 534-544.

Sosulky, F., The centrifuge method for determining flour absorption in hard red spring wheats. Cereal Chemists, 39(1), pp. 344-349, 1962.

Association of Cereal Chemistry International (AACC). Approved methods of the american of american association of cereal chemist. AACCI method pasting properties of oat - rapid viscosity analysis. St. Paul, MN: AACC, 2000.

Sívoli, L., Pérez, E. y Rodríguez, P., Análisis estructural de almidón nativo de yuca (Manihot esculenta C.) empleando técnicas morfométricas, químicas, térmicas y reológicas. Revista de la Facultad de Agronomía (LUZ), 29, pp. 293-313, 2012.

Khatoon, S., Sreerama, Y.N., Raghavendra, D., Bhattacharya, S. and Bhat, K.K., Properties of enzyme modified corn, rice and tapioca starches. Food Research International, 42, pp. 1426-1433, 2009. DOI: 10.1016/j.foodres.2009.07.025

Chen, G., and Zhang, B., Effect of resistant starch on hydrolysis and fermentation of corn starch for ethanol. Journal of Cereal Science, 56, pp. 316-320, 2012. DOI: 10.1007/s12010-009-8651-7.

Uthumporn, U., Zaidulb, I.S. and Karim, A.A., Hydrolysis of granular starch at sub-gelatinization temperature using a mixture of amylolytic enzymes. Food and Bioproducts Processing, 8(8), pp. 47-54, 2010. DOI: 10.1016/j.fbp.2009.10.001

Funa, H., Takaga, T. and Sugimoto, J., Mechanisms of saccharide polymerization and depolymerization. New York: Academic Press, 1980.

Gallant, D., Bouchet, B., Bouleon, A. and Perez, S., Physical characteristics of starch granules and susceptibility to enzyme degradation. European Journal of Clinical Nutrition, 46(2), pp. S3-S16, 1992.

Mathew, S. and Abraham, T.E., Characterization of ferulic acid incorporated starch-chitosan blend films. Food Hydrocolloids, 22(5), pp. 826-835, 2008. DOI: 10.1016/j.foodhyd.2007.03.012.

Sandhu, K. and Singh, N., Some properties of corn starches II physicochemical, gelatinization, retrogradation, pasting and textural properties. Food Chemistry, 101(4), pp. 1499-1507, 2007. DOI: 10.1016/j.foodchem.2006.01.060

Doue, G.G., Megnanou, R.M., Bedikou, E.M. and Niamke, L.S., Physicochemical characterization of starches from seven improved cassava varieties: Potentiality of industrial utilization. Journal of Applied Biosciences, 73, pp. 6002-6011, 2014.