Publicado

2018-10-01

Molecular toxicity of potential liquid membranes for lactic acid removal from fermentation broths using Lactobacillus casei ATCC 393

Toxicidad molecular de membranas liquidas potenciales para la remoción de ácido láctico a partir de caldos de fermentación con Lactobacillus casei ATCC 393

Palabras clave:

process intensification, liquid-liquid extraction, trioctylamine, tri-iso-octylamine, Aliquat 336, dodecane, dodecanol, oleyl alcohol (en)
intensificación de procesos, extracción líquida, trioctilanina, tri-iso-octilamina, Aliquat 336, dodecano, dodecanol, alcohol oleico (es)

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Toxic effects of extractants and carriers of specific microorganisms must be taken into account before using them with hybrid fermentation processes that are combined with liquid membranes or liquid-liquid extraction. In the current research three extractants (trioctylamine, tri-iso-octylamine and Aliquat 336), three diluents (dodecane, dodecanol, and oleyl alcohol) and two mixtures (extractant/diluent) were tested for molecular toxicity on the bacteria Lactobacillus casei ATCC 393 as potential components of a liquid membrane or a liquid-liquid extraction process for lactic acid removal in an intensified fermentation process. Glucose consumption, lactic acid production, and cell growth were used as toxicity indicators. Physical properties of extractants and diluents were related to the molecular toxicity on the microorganism. These results show that mixtures of tri-iso-octylamine/dodecane and trioctylamine/dodecane at a proportion of 1:9 v/v have great potential to be used in liquid membranes or liquid-liquid extraction processes on hybrid fermentations with Lactobacillus casei ATCC 393.
La toxicidad de extractantes y carriers sobre microrganismos específicos es clave a evaluar para implementar membranas líquidas o extracciones líquido-líquido integrados con fermentación. Se evaluó la toxicidad molecular de tres extractantes (trioctilamina, tri-iso-octilamina y Aliquat 336), tres diluyentes (dodecano, dodecanol y alcohol oleico) y dos mezclas (extractante/diluyente) sobre la bacteria Lactobacillus casei ATCC 393 como componentes potenciales de los procesos de separación mencionados para la remoción de ácido láctico en un proceso intensificado fermentativo. El consumo de glucosa, la producción de biomasa y de ácido láctico se usaron como indicadores de toxicidad. Las propiedades físicas de los extractantes y de los diluyentes fueron relacionadas con la toxicidad molecular sobre el microorganismo. De acuerdo a los resultados, las mezclas tri-iso-octilamina/dodecano y trioctilamina/dodecano a una proporción 1:9 v/v tienen gran potencial de ser usados en membranas líquidas o procesos de extracción líquido-líquido en fermentaciones híbridas con Lactobacillus casei ATCC 393.

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